A5040325034- Parasite Biology and Host Interactions
- Parasites and Host Interactions
- Helminth infection and control
- Mollusks and Parasites Studies
- Zoonotic diseases and public health
- Aquatic Invertebrate Ecology and Behavior
- Malaria Research and Control
- Global Maternal and Child Health
- Child Nutrition and Water Access
- Identification and Quantification in Food
- Trace Elements in Health
- MicroRNA in disease regulation
- Research on Leishmaniasis Studies
- Environmental Toxicology and Ecotoxicology
- Aquaculture disease management and microbiota
- Coccidia and coccidiosis research
- Mercury impact and mitigation studies
- vaccines and immunoinformatics approaches
- Invertebrate Taxonomy and Ecology
- Phytochemistry Medicinal Plant Applications
- Global Health and Surgery
- Mosquito-borne diseases and control
- Fish biology, ecology, and behavior
- Ubiquitin and proteasome pathways
- Phytochemistry and Bioactivity Studies
Fundação Oswaldo Cruz
2016-2025
Stanford University
2023
Câmara Municipal de Almada
2023
Instituto de Ensino e Pesquisa Santa Casa
2010
University of New Mexico
2006
Vector Oncology (United States)
2006
Abstract Biomphalaria snails are instrumental in transmission of the human blood fluke Schistosoma mansoni . With World Health Organization's goal to eliminate schistosomiasis as a global health problem by 2025, there is now renewed emphasis on snail control. Here, we characterize genome glabrata, lophotrochozoan protostome, and provide timely important information biology. We describe aspects phero-perception, stress responses, immune function regulation gene expression that support...
Seeking the identification of Angiostrongylus cantonensis as a potential etiological agent three clinical cases eosinophilic meningitis, mollusc specimens were collected in state Espírito Santo, Brazil. The snails identified Sarasinula marginata (45 specimens), Subulina octona (157), Achatina fulica (45) and Bradybaena similaris (23). Larvae obtained submitted to polymerase chain reaction restriction fragment length polymorphism diagnosis. Their genetic profile corresponded A. cantonensis....
Abstract Schistosomiasis is a neglected tropical disease caused by Schistosoma parasites. are obligate parasites of freshwater Biomphalaria and Bulinus snails, thus controlling snail populations critical to reducing transmission risk. As snails sensitive environmental conditions, we expect their distribution significantly impacted global change. Here, used machine learning, remote sensing, 30 years occurrence records map the historical current forward-transmitting hosts throughout Brazil. We...
Angiostrongylus cantonensis is the most common aetiological agent of human eosinophilic meningoencephalitis. Following a report indicating presence this parasite in Brazil 2007, present study was undertaken to investigate A. surrounding Brazilian port areas. In total, 30 ports were investigated and following molluscs identified: Achatina fulica, Belocaulus sp., Bradybaena similaris Cyclodontina Helix Leptinaria Melampus Melanoides tuberculata, Phyllocaulis Pomacea Pseudoxychona Rhinus...
Accurate diagnosis followed by timely treatment is an effective strategy for the prevention of complications together with reducing schistosomiasis transmission. Recombinase Polymerase Amplification (RPA) a simple, rapid, sensitive, and specific isothermal method low resource needs. This research aimed at development optimisation real-time (RT) lateral flow (LF) RPA assay detection Schistosoma mansoni.Recombinase reactions were performed full- (as recommended) half-volumes (to reduce costs),...
The polymerase chain reaction and restriction fragment length polymorphism (RFLP) of the internal transcribed spacer (ITS) region rRNA gene, using enzyme DdeI were used for molecular identification ten species one subspecies Brazilian Biomphalaria. Emphasis is given to analysis B. oligoza, schrammi amazonica. RFLP profiles obtained this highly distinctive majority exhibited low levels intraspecific among specimens from different regions Brazil. However, peregrina oligoza presented very...
Angiostrongylus cantonensis, A. costaricensis, and vasorum are etiologic agents of human parasitic diseases. Their identification, at present, is only possible by examining the adult worm after a 40-day period following infection vertebrate hosts with third-stage larvae. In order to obtain diagnostic tool differentiate larvae from three referred species, polymerase chain reaction-restriction fragment length polymorphism was carried out. The rDNA second internal transcribed spacer (ITS2)...
Published and unpublished observations on geographical distribution of Biomphalaria snails in the State Minas Gerais, Brazil, were compiled. This work is aimed at knowing present occurrence species this region, contributing to elaboration planorbid chart Gerais. In malacological surveys, performed by several researchers, presence seven genus was recorded. Those planorbids found 12 mesoregions, 283 (33.1%) municipalities out 853 with following distribution: B. glabrata (185 municipalities),...
Snails of the family Lymnaeidae act as intermediate hosts in biological cycle Fasciola hepatica, which is a agent fasciolosis, parasitic disease medical importance for humans and animals. The present work aimed to update map spatial distribution host snails F. hepatica Brazil. Data on lymnaeids species were compiled from Collection Medical Malacology (Fiocruz-CMM, CPqRR), (MZUSP), "SpeciesLink" (CRIA) network through systematic surveys literature. Our maps show that Pseudosuccinea columella...
Leishmaniases are serious diseases caused by trypanosomatid protozoans of the genus Leishmania transmitted bite phlebotomine sand flies. We analyzed records pertaining to Lutzomyia longipalpis (Lutz and Neiva, 1912) cruzi (Mangabeira, 1938) in Brazil from following sources: collection flies Centro de Pesquisas René Rachou/Fiocruz (FIOCRUZ-COLFLEB), "SpeciesLink" (CRIA) database, systematic surveys scientific articles gray literature (dissertations, theses, communications), disease data...
Schistosoma sp. transmission is linked to water bodies, poor sanitation, and the presence of intermediate hosts. Nevertheless, parasite detection in snails hampered by challenges snail sampling low infection rates, mainly moderate low-endemic areas, as well requiring specialized personnel being time-consuming. Thus, there a need improve tools assist schistosomiasis surveillance an environmental DNA (eDNA) approach may help overcome these limitations. Here, we standardized used eDNA-based...
O objetivo desse trabalho foi determinar a prevalência das parasitoses em escolares de Bambuí, através exames coprológicos (direto e Kato-Katz) reavaliar os criadouros moluscos descritos no município. Dos 2.901 examinados, 20,1% estavam parasitados, sendo que Giardia lamblia, Entamoeba coli, Ascaris lumbricoides ancilostomídeos foram parasitas mais freqüentes, com prevalências 6,2%, 4,8% 1,4%, respectivamente. Os significativamente freqüentes na zona rural nos alunos 14 anos, enquanto da E....
In spite of their abundance, widespread distribution and medical importance, the phylogenetic relationships among Biomphalaria snails have received relatively little attention. We collected studied 29 populations obtained from different localities Brazil. sequenced ribosomal DNA second internal transcribed spacer (ITS2) following species: B. glabrata, tenagophila tenagophila, occidentalis, straminea, peregrina, kuhniana, schrammi, amazonica, oligoza, intermedia an outgroup species Helisoma...
Studies performed in the last 30 years demonstrated that a strain of B. tenagophila from Taim Biological Reserve is completely resistant to Schistosoma mansoni infection. This resistance parasite infection dominant characteristic during crossbreeding with susceptible strains. These experiments also identified 350 bp molecular marker exclusive and does not occur other geographic strains this snail species. The (Taim/RS) Biomphalaria was bred on large scale, physically marked introduced into...
The low stringency-polymerase chain reaction (LS-PCR) and loop-mediated isothermal amplification (LAMP) assays were used to detect the presence of S. mansoni DNA in (1) Brazilian intermediate hosts ( Biomphalaria glabrata , B. straminea, tenagophila ) with patent infections, (2) snails prepatent (3) various mixtures infected noninfected snails; (4) other trematode species. showed high sensitivity specificity could when one positive snail was included a pool 1,000 negative specimens . These...
The World Health Organization (WHO) recognizes schistosomiasis as one of the Neglected Tropical Diseases targeted for global elimination in 2030 Agenda Sustainable Development Goals. In Brazil, mansoni is considered a public health problem, particularly prevalent among vulnerable populations living areas with poor environmental and sanitary conditions. 2022, WHO published Guideline encompassing recommendations to assist national programs endemic countries achieving morbidity control,...
Improvements in diagnostics for schistosomiasis both humans and snail hosts are priorities to be able reach the World Health Organization (WHO) goal of eliminating disease as a public health problem by 2030. In this context, molecular isothermal amplification tests, such Recombinase Polymerase Amplification (RPA), promising use endemic areas at point-of-need their accuracy, robustness, simplicity, time-effectiveness. The developed recombinase polymerase assay targeting Schistosoma mansoni...
The freshwater snails Biomphalaria straminea, B. intermedia, kuhniana and peregrina, are morphologically similar; based on this similarity the first three species were therefore grouped in complex straminea.The morphological identification of these is characters such as vaginal wrinkling, relation between prepuce: penial sheath:deferens vas number muscle layers penis wall.In study polymerase chain reaction restriction fragment length polymorphism technique was used for molecular...
A parasitological assay of feces, through the Kato-Katz method, was undertaken in 18,973 schoolchildren (7 to 14 years old), at primary school level, from a public State Minas Gerais. Three mesoregions were studied: Tri ngulo Mineiro/ Alto Para ba ( 60 municipalities); northwest Gerais (13) and south/south-west regions (144). Among examined children, 15,545 (82%) negative; 2,863 (15%) infected with single species helminthes 565 (3%) by more than one species. The prevalence rates were: A....
Biomphalaria tenagophila is very important for schistosomiasis transmission in Brazil. However its mechanisms of interaction with Schistosoma mansoni are still scantly studied. Since this snail displays strains highly susceptible or completely resistant to the parasite infection, knowledge that would be a useful tool understand mechanism resistance. Particularly, Taim strain consistently shows absolute resistance against trematode, and dominant character. A multidisciplinary research group...
To provide a novel resource for analysis of the genome Biomphalaria glabrata, members international glabrata Genome Initiative (<A HREF="http://biology.unm.edu/biomphalaria-genome.html">biology.unm.edu/biomphalaria-genome.html</A>), working with Arizona Genomics Institute (AGI) and supported by National Human Research (NHGRI), produced high quality bacterial artificial chromosome (BAC) library. The BB02 strain B. field isolate (Belo Horizonte, Minas Gerais, Brasil) that is susceptible to...