A5003076206- Diphtheria, Corynebacterium, and Tetanus
- Bacterial Identification and Susceptibility Testing
- Mycobacterium research and diagnosis
- Allergic Rhinitis and Sensitization
- Food Allergy and Anaphylaxis Research
- Veterinary medicine and infectious diseases
- Clostridium difficile and Clostridium perfringens research
- Asthma and respiratory diseases
- Parasites and Host Interactions
- Genomics and Phylogenetic Studies
- Parasitic Infections and Diagnostics
- SARS-CoV-2 and COVID-19 Research
- CRISPR and Genetic Engineering
- Bacteriophages and microbial interactions
- Infective Endocarditis Diagnosis and Management
- SARS-CoV-2 detection and testing
- Insect symbiosis and bacterial influences
- Molecular Biology Techniques and Applications
- Protein purification and stability
- Plant Virus Research Studies
- Streptococcal Infections and Treatments
- Historical Medical Research and Treatments
- COVID-19 diagnosis using AI
- Viral Infectious Diseases and Gene Expression in Insects
- Coccidia and coccidiosis research
Universidade Federal da Bahia
2016-2025
Hospital Ana Nery
2020-2024
Harvard University
2020-2021
Brigham and Women's Hospital
2020-2021
Hospital Vozandes
2020
Universidade Federal de Minas Gerais
2005-2012
Centro de Genética Clínica
2006
Fundação de Amparo à Pesquisa do Estado de Minas Gerais
2004
There is growing concern about individuals reported to suffer repeat COVID-19 disease episodes, these in a small number of cases characterised as de novo infections with distinct sequences, indicative insufficient protective immunity even the short term.
Corynebacterium pseudotuberculosis is the aetiological agent of caseous lymphadenitis (CLA), a debilitating disease sheep and goats. Accurate diagnosis CLA primarily relies on microbiological examination, followed by biochemical identification isolates. In an effort to facilitate C. detection, multiplex PCR (mPCR) assay was developed targeting three genes this bacterium: 16S rRNA gene, rpoB pld. This method allowed efficient 40 isolates bacterium that had been identified previously testing....
Background Corynebacterium pseudotuberculosis, a Gram-positive, facultative intracellular pathogen, is the etiologic agent of disease known as caseous lymphadenitis (CL). CL mainly affects small ruminants, such goats and sheep; it also causes infections in humans, though rarely. This species distributed worldwide, but has most serious economic impact Oceania, Africa South America. Although C. pseudotuberculosis major health productivity problems for livestock, little about molecular basis...
CRISPR (Clustered regularly interspaced short palindromic repeats)-based diagnostic technologies have emerged as a promising alternative to accelerate delivery of SARS-CoV-2 molecular detection at the point need. However, efficient translation CRISPR-diagnostic field application is still hampered by dependence on target amplification and reliance fluorescence-based results readout. Herein, an amplification-free CRISPR/Cas12a-based technology for RNA presented using smartphone camera This...
Abstract Background Bacterial exported proteins represent key components of the host-pathogen interplay. Hence, we sought to implement a combined approach for characterizing entire exoproteome pathogenic bacterium Corynebacterium pseudotuberculosis , etiological agent caseous lymphadenitis (CLA) in sheep and goats. Results An optimized protocol three-phase partitioning (TPP) was used obtain C. exoproteins, newly introduced method data-independent MS acquisition (LC-MS E ) employed protein...
Corynebacterium diphtheriae, ulcerans and pseudotuberculosis constitute a group of potentially toxigenic microorganisms that are related to different infectious processes in animal human hosts. Currently, there is lack information on the prevalence disease caused by these pathogens, which partially due reduction frequency routine laboratory testing. In this study, multiplex polymerase chain reaction (mPCR) assay can simultaneously identify determine toxigenicity corynebacterial species with...
Deep-learning (DL)-based image processing has potential to revolutionize the use of smartphones in mobile health (mHealth) diagnostics infectious diseases. However, high variability cellphone data acquisition and common need for large amounts specialist-annotated images traditional DL model training may preclude generalizability smartphone-based diagnostics. Here, we employed adversarial neural networks with conditioning develop an easily reconfigurable virus diagnostic platform that...
An 11-year-old male mixed-breed cat, with exclusively indoor life, presented 3 cough episodes after the owners tested positive by RT-PCR for SARS-CoV-2. The house is inhabited 5 people (3 adults and 2 children), of have shown mild symptoms associated throat discomfort. cat was vaccinated, had no history any previous disease, negative feline coronavirus (FCoV), immunodeficiency virus (FIV) leukaemia (FeLV). Rectal sample collected from SARS-CoV-2 RT-PCR. Viral genome sequences recovered human...
The frequency and severity of human infections associated with Corynebacterium ulcerans appear to be increasing in different countries. Here, we describe the first C. strain producing a diphtheria-like toxin isolated from an elderly woman fatal pulmonary infection history leg skin ulcers Rio de Janeiro metropolitan area.
Toxocariasis is a widespread helminth infection of dogs and cats, caused by Toxocara canis cati larvae, respectively. spp. can cause zoonotic infections in humans invading tissues organs causing pathology. larvae release excretory-secretory molecules (TES) into the body their host that are fundamental to host-parasite interaction could be used as targets for novel diagnostics vaccines. In present study, we identified 646 T. proteins from TES larval extract using 1D-SDS PAGE followed mass...
Corynebacterium ulcerans was isolated from nares of one asymptomatic dog kept in an animal shelter the metropolitan area Rio de Janeiro, Brazil. The RNA polymerase beta subunit-encoding gene sequenced to confirm species identity. C. strains producing phospholipase D, but not diphtheria toxin, are able cause severe disease humans, such as pneumonia and granulomatous nodules pulmonary tissues. infection rate varies really widely by region, probably because variations reported rates. Dogs with...
Severe asthma is a heterogeneous condition often resistant to conventional corticosteroid therapy, necessitating the identification of novel biomarkers and therapeutic targets. This study investigates immunological, transcriptional, proteomic in severe patients from Brazilian ProAR cohort. Cytokines were measured using multiplex technology (Luminex, Merck) differential sputum cell count was performed by cytospin preparations. Sputum transcriptomics RNA-seq Ion S5 next-generation sequencing...
The mite T. truncatus is a significant agricultural pest and may serve as potential vector for viral transmission. However, the virome of remains understudied. Through metatranscriptomic analyses publicly available data, we uncovered diverse range viruses associated with spider mite, including crop-infecting pathogenic species such Potato virus Y Cherry A , fourteen previously unknown across several families (e.g., Virgaviridae Dicistroviridae Kitaviridae Betaflexiviridae Nudiviridae )....
SUMMARY Invasive infections caused by Corynebacterium diphtheriae in vaccinated and non-vaccinated individuals have been reported increasingly. In this study we used multilocus sequence typing (MLST) to genetic relationships between six invasive strains of bacterium isolated solely the urban area Rio de Janeiro, Brazil, during a 10-year period. Of note, all rendered negative results PCR reactions for tox gene, four presented an atypical sucrose-fermenting ability. Five represented new types....
This study involves the comparison between exoproteomes of two different strains Corynebacterium pseudotuberculosis, etiologic agent caseous lymphadenitis in small ruminants. In a previous study, based on gel-free system (TPP-LC/MS(E)), 70 exoproteins for strain 1002 and 67 C231, totaling 93 extracellular proteins C. were identified. present work, we have used 2D gel electrophoresis to resolve both strains, which then digested with trypsin, analyzed by MALDI-TOF/TOF identified software...
Pathogenic intracellular bacteria can respond to antimicrobial mechanisms of the host cell through transient activation stress-responsive genes by alternative sigma (σ) factors RNA polymerase. We evaluated contribution extracytoplasmic function factor σ(E) for Corynebacterium pseudotuberculosis resistance stress conditions resembling those found intracellularly during infection. A sigE-null mutant strain (ΔsigE) this bacterium was more susceptible in vitro acidic pH, surface stressors, and...