A5101908133- Diphtheria, Corynebacterium, and Tetanus
- Probiotics and Fermented Foods
- Mycobacterium research and diagnosis
- Veterinary medicine and infectious diseases
- Bacterial Identification and Susceptibility Testing
- Gut microbiota and health
- Digestive system and related health
- Bacteriophages and microbial interactions
- Genomics and Phylogenetic Studies
- Clostridium difficile and Clostridium perfringens research
- Viral gastroenteritis research and epidemiology
- Infant Nutrition and Health
- Escherichia coli research studies
- Helicobacter pylori-related gastroenterology studies
- Antimicrobial Peptides and Activities
- Inflammatory Bowel Disease
- Microbial Metabolism and Applications
- Milk Quality and Mastitis in Dairy Cows
- Immune Response and Inflammation
- Cancer Research and Treatments
- Bacterial Genetics and Biotechnology
- Enzyme Production and Characterization
- Pediatric health and respiratory diseases
- vaccines and immunoinformatics approaches
- Pharmacological Effects of Natural Compounds
Saga University
2005-2025
Universidade Federal de Minas Gerais
2014-2023
Hokkaido University Hospital
2016
Institut des Sciences Biologiques
2014-2015
Kyoto University
1966-2014
Centro de Genética Clínica
2006-2014
Consejo Nacional de Investigaciones Científicas y Técnicas
2014
Instituto de Ciencias Tecnológicas
2014
Center for Effective Philanthropy
2012
Universidade Federal do Pará
2011
Loss of E-cadherin (E-cad) triggers invasion, metastasis, and dedifferentiation in various epithelial carcinomas. Recently, it has been reported that two transcription factors, Snail SIP1 (Smad interacting protein 1), directly repress the E-cad gene by binding E-box on promoter. Our aim is to solve molecular mechanism hepatocellular carcinoma (HCC). We first showed an inverse correlation between Snail/SIP 1 expression among five HCC lines with different phenotypes. The result indicated...
We have previously demonstrated in an vitro study that Snail increased the invasion activity of hepatoma cells by upregulating matrix metalloproteinase (MMP) gene expression. In present study, we examined whether expression correlates with cancer and prognosis patients hepatocellular carcinoma (HCC). Quantitative reverse transcription–polymerase chain reaction (RT–PCR) was performed to evaluate Snail, E-cadherin, MMP mRNA expressions eight nodule-in-nodule tumours 47 ordinary HCC tissues....
Lactococcus lactis, the model lactic acid bacterium (LAB), is a food grade and well-characterized Gram positive bacterium. It good candidate for heterologous protein delivery in foodstuff or digestive tract. L. lactis can also be used as producer fermentor. Many proteins have already been produced but only few reports allow comparing production yields given either intracellularly secreted medium. Here, we review several works evaluating influence of localization on lactis. The questions size...
Corynebacterium pseudotuberculosis is the aetiological agent of caseous lymphadenitis (CLA), a debilitating disease sheep and goats. Accurate diagnosis CLA primarily relies on microbiological examination, followed by biochemical identification isolates. In an effort to facilitate C. detection, multiplex PCR (mPCR) assay was developed targeting three genes this bacterium: 16S rRNA gene, rpoB pld. This method allowed efficient 40 isolates bacterium that had been identified previously testing....
Corynebacterium diphtheriae is one of the most prominent human pathogens and causative agent communicable disease diphtheria. The genomes 12 strains isolated from patients with classical diphtheria, endocarditis, pneumonia were completely sequenced annotated. Including genome C. NCTC 13129, we herewith present a comprehensive comparative analysis 13 first characterization pangenome species diphtheriae. Comparative genomics showed extensive synteny revealed core consisting 1,632 conserved...
Bacteria are highly diverse organisms that able to adapt a broad range of environments and hosts due their high genomic plasticity. Horizontal gene transfer plays pivotal role in this genome plasticity evolution by leaps through the incorporation large blocks sequences, ordinarily known as islands (GEIs). GEIs may harbor genes encoding virulence, metabolism, antibiotic resistance symbiosis-related functions, namely pathogenicity (PAIs), metabolic (MIs), (RIs) symbiotic (SIs). Although many...
Background Corynebacterium pseudotuberculosis, a Gram-positive, facultative intracellular pathogen, is the etiologic agent of disease known as caseous lymphadenitis (CL). CL mainly affects small ruminants, such goats and sheep; it also causes infections in humans, though rarely. This species distributed worldwide, but has most serious economic impact Oceania, Africa South America. Although C. pseudotuberculosis major health productivity problems for livestock, little about molecular basis...
Corynebacterium pseudotuberculosis is a facultative intracellular pathogen and the causative agent of several infectious contagious chronic diseases, including caseous lymphadenitis, ulcerative lymphangitis, mastitis, edematous skin disease, in broad spectrum hosts. In addition, infections pose rising worldwide economic problem ruminants. The complete genome sequences 15 C. strains isolated from different hosts countries were comparatively analyzed using pan-genomic strategy. Phylogenomic,...
Lactic acid bacteria (LAB) represent a heterogeneous group of microorganisms that are naturally present in many foods and possess wide range therapeutic properties. The aim this paper is to an overview the current expanding knowledge one mechanisms by which LAB other probiotic participate prevention treatment gastrointestinal inflammatory disease through their immune-modulating A special emphasis will be placed on critical role anti-inflammatory cytokine IL-10, brief uses genetically...
Vibrio cholerae is the causal organism of cholera epidemic, which mostly prevalent in developing and underdeveloped countries. However, incidences developed countries are also alarming. Because emergence new drug-resistant strains, even though several generic drugs vaccines have been over time, infections remain a global health problem that appeals for development novel against pathogen. Here, applying comparative proteomic reverse vaccinology approaches to exoproteome secretome pathogen, we...
Many probiotic bacteria have been described as promising tools for the treatment and prevention of inflammatory bowel diseases (IBDs). Most these are lactic acid bacteria, which part healthy human microbiota. However, little is known about effects transient present in normal diets, including Lactococcus lactis.In study, we analysed immunomodulatory three L. lactis strains vitro using intestinal epithelial cells. NCDO 2118 was administered 4 days to C57BL/6 mice during remission period...
Reactive oxygen species, such as hydrogen peroxide (H2O2), are involved in various aspects of tumour development. Decreasing their levels can therefore be a promising approach for colon cancer prevention. The objective this study was to evaluate the effect catalase-producing Lactococcus lactis on prevention an experimental murine 1,2-dimethylhydrazine (DMH)-induced cancer. DMH-treated BALB/c mice received either L. strain or isogenic non-catalase-producing control, whereas other untreated...
Abstract Background Corynebacterium pseudotuberculosis is generally regarded as an important animal pathogen that rarely infects humans. Clinical strains are occasionally recovered from human cases of lymphadenitis, such C. FRC41 was isolated the inguinal lymph node a 12-year-old girl with necrotizing lymphadenitis. To detect potential virulence factors and corresponding gene-regulatory networks in this isolate, genome sequence FCR41 determined by pyrosequencing functionally annotated....
Abstract Background Bacteria may compete with yeast for nutrients during bioethanol production process, potentially causing economic losses. This is the first study aiming at quantification and identification of Lactic Acid (LAB) present in industrial processes different distilleries Brazil. Results A total 489 LAB isolates were obtained from four 2007 2008. The abundance fermentation tanks varied between 6.0 × 10 5 8.9 8 CFUs/mL. Crude sugar cane juice contained 7.4 7 to CFUs. Most belonged...
Abstract Background Corynebacterium ulcerans has been detected as a commensal in domestic and wild animals that may serve reservoirs for zoonotic infections. During the last decade, frequency severity of human infections associated with C. appear to be increasing various countries. As knowledge genes contributing virulence this bacterium was very limited, complete genome sequences two strains metropolitan area Rio de Janeiro were determined characterized by comparative genomics: 809...
Lactococci are noninvasive bacteria frequently used as protein delivery vectors and, more recently, in vitro and vivo DNA vehicles. We previously showed that a functional eukaryotic enhanced green fluorescent (eGFP) expression plasmid vector was delivered epithelial cells by Lactococcus lactis producing Listeria monocytogenes internalin A (L. InlA(+)), but this strategy is limited to transgenic mice guinea pigs. In study, we compare the internalization ability of L. InlA(+) either...
The adaptability of pathogenic bacteria to hosts is influenced by the genomic plasticity bacteria, which can be increased such mechanisms as horizontal gene transfer. Pathogenicity islands play a major role in this type transfer because they are large, horizontally acquired regions that harbor clusters virulence genes mediate adhesion, colonization, invasion, immune system evasion, and toxigenic properties acceptor organism. Currently, pathogenicity mainly identified silico based on various...
The intestinal microbiota plays an important role in human health through the modulation of innate immune responses. While selected commensal bacteria are marketed specific probiotic products to control these responses, relatively little is known about potential dairy that have principally been for their fermentation properties. responses may reduce chronic inflammation inflammatory bowel diseases like ulcerative colitis.A collection Lactobacillus delbrueckii strains was screened effects...