A5004148807- Ubiquitin and proteasome pathways
- Research on Leishmaniasis Studies
- Protein Degradation and Inhibitors
- Endoplasmic Reticulum Stress and Disease
- Heat shock proteins research
- Crystallization and Solubility Studies
- Antimicrobial Peptides and Activities
- Trypanosoma species research and implications
- Glycosylation and Glycoproteins Research
- X-ray Diffraction in Crystallography
- Biochemical and Structural Characterization
- Amino Acid Enzymes and Metabolism
- Blood Coagulation and Thrombosis Mechanisms
- Polyamine Metabolism and Applications
- Genetic and phenotypic traits in livestock
- Genetic Mapping and Diversity in Plants and Animals
- Genetics and Plant Breeding
- Mitochondrial Function and Pathology
- Protease and Inhibitor Mechanisms
- Parasites and Host Interactions
- Cancer Research and Treatments
- Histone Deacetylase Inhibitors Research
- vaccines and immunoinformatics approaches
- NF-κB Signaling Pathways
- Renin-Angiotensin System Studies
Universidade de São Paulo
2010-2024
Universidade Federal de São Carlos
2015-2024
Universidade Federal do Piauí
2021
University of Cambridge
2016-2019
Universidade Federal de Viçosa
2006-2016
Universidade de Ribeirão Preto
2013
Zero to Three
2010
AgroBio
2008
Ticks cause massive damage to livestock and vaccines are one sustainable substitute for the acaricides currently heavily used control infestations. To guide antigen discovery a vaccine that targets gamut of parasitic strategies mediated by tick saliva enables immunological memory, we exploited transcriptome constructed from salivary glands all stages Rhipicephalus microplus ticks feeding on genetically tick-resistant susceptible bovines. Different levels host anti-tick immunity affected gene...
Fbxo7 is a clinically relevant F-box protein, associated with both cancer and Parkinson's disease (PD). Additionally, SNPs within FBXO7 are correlated alterations in red blood cell parameters. Point mutations map specific functional domains, including near its domain substrate recruiting suggesting that deficiencies SCFFbxo7/PARK15 ubiquitin ligase activity mechanistically linked to early-onset PD. To date, relatively few substrates of the have been identified. These include HURP (hepatoma...
In this paper, a series of new ruthenium complexes the general formula [Ru(NS)(dpphpy)(dppb)]PF6 (Ru1–Ru3), where dpphpy = diphenyl-2-pyridylphosphine, NS ligands 2-thiazoline-2-thiol (tzdt, Ru1), 2-mercaptopyrimidine (pySm, Ru2), and 4,6-diamino-2-mercaptopyrimidine (damp, Ru3), dppb 1,4-bis(diphenylphosphino)butane, were synthesized characterized by elemental analysis, spectroscopic techniques (IR, UV/visible, 1D 2D NMR), X-ray diffraction. characterization, correlation between phosphorus...
Abstract Background In hematologic cancers, including leukemia, cells depend on amino acids for rapid growth. Anti-metabolites that prevent their synthesis or promote degradation are considered potential cancer treatment agents. Amino acid deprivation triggers proliferation inhibition, autophagy, and programmed cell death. l -lysine, an essential acid, is required tumor growth has been investigated its as a target treatment. -lysine α-oxidase, flavoenzyme degrades studied ability to induce...
G protein-coupled receptors (GPCRs) are involved in essentially all physiological processes mammals. The classical GPCR signal transduction mechanism occurs by coupling to protein, but it has recently been demonstrated that interaction with β-arrestins leads activation of pathways independent the protein pathway. Also, reported some ligands can preferentially activate one these signaling pathways; being therefore called biased agonists for or β-arrestin pathways. angiotensin II (AngII) AT1...
Mounting evidence suggest that tissue levels of angiotensin (ANG) II are maintained in animals submitted to chronic angiotensin-converting enzyme (ACE) inhibitor treatment. We examined the expression transcripts for elastase-2, a chymostatin-sensitive serine protease identified as alternative pathway ANG generation from I rat vascular and relative role ACE-dependent -independent pathways generating isolated carotid artery rings spontaneously hypertensive rats (SHR) Wistar normotensive (WNR)...
Abstract Bioprospection of natural compounds to be used as plant protection offers exciting possibilities in agribusiness. ‘Samara’ (SM) and ‘Sunshine’ (SH) cultivars bell pepper are resistant against some pathogens, leaf antimicrobial peptides could account for their defence system. In the present study, 5–7 kDa were partially purified from cell wall extract (CWE) leaves further resolved by ion‐exchange chromatography. The cationic anionic peptide‐enriched fractions both SM‐CWE SH‐CWE...
The serine/arginine protein kinases respond to the EGFR-PI3K-AKT signaling module in context of pre-mRNA alternative splicing regulation. These enzymes (notably SRPK1 and SRPK2) have been found dysregulated a variety cancers, which suggests them as promising drug targets oncology. SRPK2 has related leukemia cells proliferation preferentially overexpressed T-cell acute lymphoblastic (T-ALL). Previously, synergistic combination between vincristine SRPK inhibitors observed vitro. Herein we...
Skp1, Cul1, Rbx1, and the FBXO25 protein form a functional ubiquitin ligase complex. Here, we investigate cellular distribution of its colocalization with some nuclear proteins by using immunochemical biochemical approaches. was monitored affinity-purified antibodies raised against recombinant fragment spanning residues 2-62 sequence. expressed in all mouse tissues tested except striated muscle, as indicated immunoblot analysis. Confocal analysis revealed that endogenous partially...
Ubiquitously eXpressed Transcript isoform 2 (UXTV2) is a prefoldin-like protein involved in NF-κB signaling, apoptosis, and the androgen estrogen response. UXT-V2 cofactor transcriptional enhanceosome, its knockdown inhibits TNF-α -induced activation. Fbxo7 an F-box that interacts with SKP1, Cullin1 RBX1 proteins to form SCF(Fbxo7) E3 ubiquitin ligase complex. negatively regulates signaling through TRAF2 cIAP1 ubiquitination.We combine co-immunoprecipitation, ubiquitination vitro vivo,...
The aim of the present study was to propose and evaluate use factor analysis (FA) in obtaining latent variables (factors) that represent a set pig traits simultaneously, for genome-wide selection (GWS) studies. We used crosses between outbred F2 populations Brazilian Piau X commercial pigs. Data were obtained on 345 pigs, genotyped 237 SNPs, with 41 traits. FA allowed us obtain four biologically interpretable factors: "weight", "fat", "loin", "performance". These factors as dependent...
Abstract Some varieties of eggplant are little susceptible to some plant pathogens, and antimicrobial peptides (AMPs) could be part this innate‐defence mechanism. This work aimed evaluate the in vitro activity peptide‐enriched fractions from cell wall extracts (CWE) soluble (SE) young plants fully expanded leaves eggplants against Ralstonia solanacearum Clavibacter michiganensis ssp. growth. The results indicated that highest inhibition level multiplication both bacteria was obtained with...
Abstract In cancer, many genes are mutated by genome rearrangement, but our understanding of the functional consequences this remains rudimentary. Here we report F-box protein encoded FBXL17 is disrupted in region gene that encodes its substrate-binding leucine rich repeat (LRR) domain. Truncating Fbxl17 LRRs impaired association with other SCF holoenzyme subunits Skp1, Cul1 and Rbx1, decreased ubiquitination activity. Loss also differentially affected binding to targets. Thus, genomic...
Cullin-1-RING ubiquitin ligases (CRL1) or SCF1 (SKP1-CUL1-RBX1) E3 are the largest and most extensively investigated class of in mammals that regulate fundamental processes, such as cell cycle proliferation. These enzymes multiprotein complexes comprising SKP1, CUL1, RBX1, an F-box protein acts a specificity factor by interacting with SKP1 through its domain recruiting substrates via other domains. important players ubiquitination process, recognizing transferring to destined for degradation...
FBXO25 is one of the 68 human F-box proteins that serve as specificity factors for a family ubiquitin ligases composed s-phase-kinase associated protein 1, really interesting new gene-box Cullin and (SCF1) are involved in targeting destruction across proteasome system. We recently reported accumulates novel subnuclear structures named FBXO25-associated nuclear domains (FAND). Combining two-step affinity purification followed by MS with classical two-hybrid screen, we identified 132 potential...
Visceral leishmaniasis (VL) is a neglected disease considered serious public health problem, especially in endemic countries. Several studies have discovered monoxenous trypanosomatids (Leptomonas and Crithidia) patients with VL. In different situations of leishmaniasis, investigations examined cases co-infection between Leishmania spp. Crithidia These coinfections been observed wide range vertebrate hosts, indicating that they are not rare. Diagnostic techniques require improvements more...