To verify the presence of cross-reaction among leishmaniosis, ehrlichiosis and babesiosis in serological diagnostics used human visceral leishmaniasis control programs, serum samples from endemic non-endemic areas were collected tested by Indirect Fluorescent Antibody (IFAT) Enzyme-linked immunosorbent assay (ELISA). All positive for Leishmania sp., ELISA IFAT, 51% Babesia canis 43% Ehrlichia IFAT. None but 67% B. 78% E. using same test. When four area positive. These dogs then located no...

The genus Sarcocystis and the species Toxoplasma gondii are most prevalent sarcocystid organisms found in birds. Molecular phylogenies based on first internal transcribed spacer of ribosomal coding DNA (ITS1) have been widely used to identify them. Here, pectoral muscles from 400 wild birds Brazil were screened by means molecular methods using nested PCR, Sanger sequencing yielded amplicons. A pan-sarcocystid ITS1-directed PCR revealed 28 infected falcatula (ten Piciformes, eight...

Leishmaniases are important neglected tropical diseases caused by protozoans of the genus Leishmania spp. The female sandflies bite (Diptera: Psychodidae: Phlebotominae) is main transmission form in epidemiological chains these diseases. Thus, studies on Phlebotominae subfamily fundamental to improve comprehension leishmaniases epidemiology, revealing relationships dipterans with parasite and reservoirs. An entomological survey Phlebotomine was carried out investigate fauna, DNA detection...

O objetivo da presente pesquisa foi avaliar comparativamente os métodos diagnósticos Leishmaniose Visceral Canina (LVC), utilizando-se o ensaio imunoenzimático indireto (ELISA), a reação de imunofluorescência indireta (RIFI), histoquímica (HE) e imunoistoquímica (IMIQ) em tecidos órgãos, como baço, linfonodo fígado.Além disso, Reação Cadeia pela Polimerase (PCR) das amostras sangue dos utilizada para comparar confirmar negativos não conclusivos pelos acima.Para esse estudo, foram utilizados...

Leishmaniasis is a zoonotic disease that affects 12 million people worldwide. Several mammalian species can serve as reservoir for this disease. Dogs are the main visceral leishmaniasis in urban areas, which has become serious public health concern Brazil. The aim of study was to evaluate presence Leishmania spp. captive wild animals from Ilha Solteira, São Paulo, Blood and various tissues samples were collected five different species: Speothos venaticus, Chrysocyon brachyurus, Cerdocyon...

The relevance of the dog as a source visceral leishmaniasis infection is known, but role cats reservoir hosts for not yet fully clear. This study assessed efficacy conjunctival swab PCR (CS-PCR) in detection infected by Leishmania spp. results were seven (13.5%) positive PCR, 52 tested from Pirassunuga-SP and Ilha Solteira-SP. From city Pirassununga - SP 28.6% (2/7) Solteira 11.1% (5/45) positive. showed that CS-PCR was capable detecting this protozoan. Conjunctival samples proved easier to...

Abstract In this study, an evaluation was made of three treatments against feline leishmaniosis (FeL) and their impacts on the transmission Leishmania infantum to its vector, Lutzomyia longipalpis. A cat with clinical signs FeL examined L. diagnosed. Subsequently, subjected xenodiagnosis detected in vectors. The then treated different drugs improvement parasite transmissibility vector were evaluated. Promastigotes observed 21/52 female sandflies (40.38%) a prior treatments. Clinical...

INTRODUCTION: Conjunctival swab PCR was evaluated as a tool to diagnose visceral leishmaniasis in dogs. METHODS: compared indirect immunofluorescence antibody test and blood PCR. RESULTS: Indirect significantly correlated with conjunctival (p < 0.05), but not > 0.05). In addition, associated presence of clinical symptoms whereas absence CONCLUSIONS: Results indicate that is useful epidemiological surveys canine leishmaniasis.

O objetivo da presente pesquisa foi avaliar comparativamente os métodos diagnósticos Leishmaniose Visceral Canina (LVC), utilizando-se o ensaio imunoenzimático indireto (ELISA), a reação de imunofluorescência indireta (RIFI), histoquímica (HE) e imunoistoquímica (IMIQ) em tecidos órgãos, como baço, linfonodo fígado. Além disso, Reação Cadeia pela Polimerase (PCR) das amostras sangue dos utilizada para comparar confirmar negativos não conclusivos pelos acima. Para esse estudo, foram...

In visceral leishmaniasis, the detection of agent is paramount importance to identify reservoirs infection. Here, we evaluated diagnostic attributes PCRs based on primers directed cytochrome-B (cytB), cytochrome-oxidase-subunit II (coxII), cytochrome-C (cytC), and minicircle-kDNA. Although cytB, coxII, cytC were able detect different species Leishmania, nucleotide sequence their amplicons allowed unequivocal differentiation species, analytical sensitivity these much lower than kDNA-PCR....

Giardia duodenalis is divided into eight assemblages (named A to H). Isolates of assemblage are four sub-assemblages (AI, AII, AIII and AIV). While isolates sub-assemblage AII almost exclusively detected in human hosts, B encountered a multitude animal hosts humans. Here, we isolated single cysts G. from stool sample found that one them had overlaps alleles an unexpectedly high number variants the beta-giardin (Bg) GLORF-C4 (OrfC4) alleles. In addition, Bg cyst fragment interspersed with...

The aim of this study was to compare molecular tests used diagnose Leishmania spp. in dogs with different stages infection. Blood and conjunctival swab (CS) samples from classified four clinical were subjected PCR protocols (13A/13B, MC1/MC2, LITSR/L5.8S LEISH-1/LEISH-2 primers). To the study, 22.3% (48/215) as without signs, 67.5% (145/215) stage I (mild disease), 7.0% (15/215) II (moderate disease) 3.2% (7/215) III (severe disease). results showed that blood samples, 13A/13B detected a...

We sought to determine risk factors (RFs) associated with the presence of antibodies against Leishmania in dogs from a rural area Ilha Solteira, SP, Brazil.Serum samples were collected 250 and tested using indirect enzyme-linked immunosorbent assay (ELISA) immunofluorescence antibody tests (IFATs). Data concerning dogs, their environment, owners' knowledge leishmaniasis questionnaire. To RFs for contact parasite, univariate statistical analysis based on chi-squared Fisher's exact tests,...

Leishmaniasis is a neglected tropical disease that continues to pose serious public health problem. Albeit dogs have long been held as the major reservoirs of Leishmania infantum, involvement domestic cats in zoonotic cycle visceral leishmaniasis has gained prominence. Here, 240 were evaluated by clinical signs and haematological/biochemical changes compatible with diagnosed serological, molecular, parasitological techniques. Thus, four naturally infected L. infantum submitted xenodiagnosis....

One hundred and sixty-six cats from two animal shelters were subjected to enzyme-linked immunosorbent assay (ELISA), indirect immunofluorescence antibody test (IFAT), conventional polymerase chain reaction (cPCR), quantitative PCR (qPCR) parasitological tests (PA) for the diagnosis of Leishmania spp. Among them, 15% (25/166), 53.6% (89/166), 3.6% (06/166) 1.8% (03/166) positive by ELISA, IFAT, both PCRs PA, respectively. The sequencing ITS-1 amplicons revealed a 100% match with infantum....

An epidemiological Leishmania spp. and entomological Phlebotomine sandflies survey was performed in cat shelters at leishmaniasis endemic area of Brazil. Blood conjunctival swab (CS) samples were collected from 94 cats two animal protection shelters. These subjected to serological tests using the indirect immunofluorescence antibody test (IFAT) enzyme-linked immunosorbent assay (ELISA) molecular by polymerase chain reaction (PCR). In addition, a same The analyses revealed positivity 31.91%...

Abstract We examined the presence of antibodies against parasites Toxoplasma gondii, Neospora caninum, and Leishmania spp., as well DNA from in dogs Pirassununga - SP. The seropositivity rate was compared with animals’ originating location. Three hundred seventy-three blood samples county’s kennel local veterinary clinics were collected analyzed. A total 300 tested for T. gondii N. caninum using an indirect immunofluorescence antibody test (IFAT); 45% (135/300) positive 24.3% (73/300)...

Visceral leishmaniasis (VL) is a widely spread zoonotic disease. In Brazil the disease caused by Leishmania (Leishmania)infantum chagasi. Peridomestic sandflies acquire etiological agent feeding on blood of infected reservoir animals, such as dogs or wildlife. The endemic in and epidemic foci have been reported densely populated cities all over country. Many clinical features infection are related to host-parasite relationship, many candidate virulence factors parasites that cause VL studied...

Abstract Leishmania spp. are important agents of human and animal leishmaniases that have an impact on public health. In this study, we aimed to detect the circulation in cattle from a visceral leishmaniasis non-endemic area state São Paulo, Brazil. DNA was extracted blood samples 100 heifers municipality Pirassununga amplified using primers specific for first internal transcriber spacer (ITS1), assess presence trypanosomatids. The assays revealed one sample presented bands between 300 350...

This study aimed to express a recombinant A2 family protein of Leishmania chagasi, Jaboticabal strain; test this as an antigen in serological assays; and investigate its antigenicity immunogenicity. A coded by allele the gene isolated from L. chagasi was expressed three different strains Escherichia coli. We used 29 sera samples Leishmune-vaccinated dogs, 482 dogs endemic areas (positive controls), 170 non-endemic (negative controls) ELISA tests using soluble Leishmaniaantigen (SLA) His-A2...

Visceral leishmaniasis (VL) is a neglected disease considered serious public health problem, especially in endemic countries. Several studies have discovered monoxenous trypanosomatids (Leptomonas and Crithidia) patients with VL. In different situations of leishmaniasis, investigations examined cases co-infection between Leishmania spp. Crithidia These coinfections been observed wide range vertebrate hosts, indicating that they are not rare. Diagnostic techniques require improvements more...

This study was about a semi-quantitative analysis of T lymphocytes (CD4+ and CD8+, FoxP3+ regulatory cells), macrophages in the gut wall dogs naturally infected with Leishmania infantum. Thirteen were divided into three groups: group 1 (G1, n=5), canine visceral leishmaniasis (CVL) L. infantum amastigotes intestine; 2 (G2, CVL but without intestinal amastigotes; 3 (G3, n=3), uninfected (control group). There no significant difference (p ≥ 0.05) on CD4+ Treg cell numbers among groups, whereas...

A mathematical model was developed to describe the dynamics of primary infection dengue virus in infant who were born a mother immune some serotype virus. The is given by system nonlinear ordinary differential equations with time-dependent variables for number DENV antibodies transferred from their immune, uninfected and infected monocytes analysis carried out where conditions existence disease-free equilibrium endemic established. numerical simulations performed considering different...

This experiment was conducted to evaluate the use of different energy levels and amino acid recommendations on carcass meat quality broilers from 42 57 days age. It used 1,600 one-day old male (Cobb) in a completely randomized design arranged factorial scheme 2x4 with 3,200 3,600 kcal ME kg-1 four feed programs. The experimental period From 1 41 age all birds received same diets based corn soybean meal. Data yield (output carcass, relation weight breast thigh overthigh weight) muscle (color,...