A5019025419- Mosquito-borne diseases and control
- Research on Leishmaniasis Studies
- Parasitic Diseases Research and Treatment
- Parasite Biology and Host Interactions
- Malaria Research and Control
- Trypanosoma species research and implications
- Insect and Pesticide Research
- Viral Infections and Vectors
- Vector-borne infectious diseases
- Parasites and Host Interactions
- Vector-Borne Animal Diseases
- Insect symbiosis and bacterial influences
- Insect and Arachnid Ecology and Behavior
- Insect Pest Control Strategies
- Animal Disease Management and Epidemiology
- Indigenous Health and Education
- Invertebrate Immune Response Mechanisms
- Insect Resistance and Genetics
- Insect Utilization and Effects
- Parasitic infections in humans and animals
- Freshwater macroinvertebrate diversity and ecology
- Food Security and Health in Diverse Populations
- Study of Mite Species
- Environmental Toxicology and Ecotoxicology
- Leptospirosis research and findings
Universidade Federal de Rondônia
2014-2025
Amazon (United States)
2010-2025
Fundação Oswaldo Cruz
2015-2024
National Institute of Amazonian Research
2002-2024
Weatherford College
2024
United States Department of State
2023
Federal University of São João del-Rei
2014
Institute of Entomology
2014
Universidade do Estado do Amazonas
2010-2013
Universidade Federal do Amazonas
2010
Abstract Large-scale surveillance and informed vector control approaches are urgently needed to ensure that national malaria programs remain effective in reducing transmission and, ultimately, achieving elimination targets. In South America, Anopheles darlingi is the primary responsible for majority of Plasmodium species transmission. However, little known about molecular markers associated with insecticide resistance this species. study, we developed a low-cost, high throughput amplicon...
Rondônia State has been subject to a higher percentage of deforestation than any other Brazilian state in the Amazon basin. Rondônia's protected forests are extremely important because their biodiversity attests species richness that lost human encroachment. Phlebotomine sand flies Leishmania vectors have potential function as environmental bioindicators. A fly survey was conducted near Brazil-Bolivian border, ombrophylous forest Guajará-Mirim Park, which is located between municipalities...
Culicoides biting midges are small insects that proven vectors of pathogens cause disease in animals and humans. There 1,368 species the world, including 149 Brazil 122 Brazilian Amazon Basin. This study documents specimens were collected between 2013 2015 municipalities Alvorada d'Oeste, Buritis, Cacoal, Costa Marques, Espigão Guajará-Mirim, Pimenta Bueno, Porto Velho São Francisco Guaporé. Collections performed using HP light traps forest, pasture peridomicilie environments.Species newly...
ABSTRACT Gamete surface protein P48/45 has been shown to be important for male gamete fertility and a strong candidate the development of malaria transmission-blocking vaccine (TBV). However, TBV Plasmodium vivax homolog Pvs48/45 slow because number challenges: availability conformationally suitable recombinant protein; lack an in vivo challenge model; inability produce P. gametocytes culture test activity antibodies. To support ongoing efforts develop as potential candidate, we initiated...
Sand flies (Diptera: Psychodidae) are responsible for the transmission of protozoan parasites that cause leishmaniases. They found predominantly in forests, but some species exploit environments have been subject to deforestation and subsequent human colonization. Studies conducted Brazil over past 30 years show adapting peri-urban urban settings. We evaluated sand fly diversity abundance rural settlement Rio Pardo, Presidente Figueiredo Municipality, Amazonas State, Brazil. Settlement...
In this study, we identified the phlebotomine sandfly vectors involved in transmission of American Cutaneous Leishmaniasis (ACL) Assis Brasil, Acre, Brazil, which is located on Brazil-Peru-Bolivia frontier. The genotyping Leishmania phlebotomines was performed using polymerase chain reaction (PCR) and PCR-restriction fragment length polymorphism. A total 6,850 sandflies comprising 67 species were captured by CDC light traps rural areas municipality. Three found state Acre for first time:...
The state of Rondônia (RO) is a hot spot for human cases cutaneous leishmaniasis. Many sandfly species in RO are putative vectors leishmaniasis.This study examines the diversity patterns and presence Leishmania DNA blood meal sources sandflies RO.A survey was performed between 2016 2018 10 municipalities categorised into three different environment types: (i) Conservation Unit (CUN) - comprised preserved ombrophilous forests; (ii) Forest Edge (FE) small forest fragments; (iii) Peridomicile...
is a major malaria vector in the Amazon region and, like other vectors, harbors community of microorganisms with which it shares network interactions. Here, we describe diversity and bacterial composition from midguts salivary glands lab-reared field-captured
Rondonia state reports more spotted fever (SF) cases than any other in the Brazilian Amazon. Ariquemes is only municipality that has reported SF this state, but no information been obtained about tick and Rickettsia spp. responsible. This study aimed to describe free-living fauna search for rickettsial DNA ticks collected from a region where one specific infection was most likely acquired. Ticks were at four sites municipality, site both Alto Paraíso Rio Crespo municipalities. A total of...
Anopheles darlingi mosquitoes are the main malaria vectors in Amazon region and play a significant role high transmission cycle of Plasmodium vivax, particularly areas undergoing degradation Amazon. Establishing an An. colony under laboratory conditions allows for critical studies on this vector, including insecticide resistance, vector competence, development new tools controlling vivax malaria. However, establishment mass-rearing mosquito colonies has proven challenging, with success being...
The mass rearing of anopheline mosquitoes under laboratory conditions is essential for advancing malaria research. It facilitates in-depth studies on mosquito biology, behavior, and genetics their role in Plasmodium transmission. However, the colonization Neotropical anophelines such as Anopheles darlingi-a primary vector Amazon region-has proven particularly challenging due to its unique reproductive characteristics. Unlike other species that can initially be colonized using forced...
This study was undertaken to investigate the prevalence of Mansonella ozzardi infection and estimate parasitic rate (PIR) in simuliid black flies municipality Pauini, Amazonas, Brazil. We used thick blood films examine 921 individuals 35 riverine communities along Pauini Purus Rivers. Simuliids were caught several communities. Flies identified, stained with haematoxylin dissected. Overall, 44 (24.86%) 177 riverines infected on River 183 (24.19%) 744 Purus. The higher men (31.81% 29.82%) than...
Abstract Background Psorophora mosquitoes are exclusively found in the Americas and have been associated with transmission of encephalitis West Nile fever viruses, among other arboviruses. Mosquito salivary glands represent final route differentiation many parasites. They also secrete molecules powerful pharmacologic actions that modulate host hemostasis, inflammation, immune response. Here, we employed next generation sequencing proteome approaches to investigate for first time composition...
Phlebotomine sand flies (Diptera: Psychodidae) are insects of medical importance due to the role that some species play in transmission leishmaniasis. This work aimed study ecological aspects among fauna inhabiting two different environments: várzea (lowland Amazonian forest) and terra firme (upland forest), both located Tefé Municipality, Amazonas State, Braziland detect Leishmania infection those phlebotomine populations. Sand were collected using HP light traps. Collection took place over...
We obtained ribosomal and mitochondrial DNA sequences from residents of Amazonas state, Brazil, with Mansonella parasitemias. Phylogenetic analysis these confirm that M. ozzardi perstans parasites occur in sympatry reveal the close relationship between Africa providing insights into parasite's New World origins.
The lack of highly-productive Nyssorhynchus darlingi laboratory colonies limits some studies. We report the first well-established colony Ny. in Brazil.Mosquitoes were collected from Porto Velho and reared at Laboratory Fiocruz/RO. After induced mating by light stimulation F1 to F6, subsequent generations free mating. Larvae distilled water fed daily until pupation.In 11 generations, produced a high number pupae after F5 generation.These results demonstrate potential for permanently...
Despite the broad distribution of M. ozzardi in Latin America and Caribbean, there is still very little DNA sequence data available to study this neglected parasite's epidemiology. Mitochondrial (mtDNA) sequences, especially cytochrome oxidase (CO1) gene's barcoding region, have been targeted successfully for filarial diagnostics epidemiological, ecological evolutionary studies. MtDNA-based studies can, however, be compromised by unrecognised mitochondrial pseudogenes, such as Numts. Here,...
Individuals with asymptomatic infection due to Plasmodium vivax are posited be important reservoirs of malaria transmission in endemic regions. Here we studied a cohort P . patients suburban area the Brazilian Amazon. Overall 1,120 individuals were screened for and 108 (9.6%) had parasitemia detected by qPCR but not microscopy. Asymptomatic higher levels antibodies against similar hematological biochemical parameters compared uninfected controls. Blood from very low transmitted main local...
The maintenance of a highly productive colony anopheline mosquitoes requires standardized methods in order to obtain sufficient number homogeneous individuals for malaria research. In this context, nutritional status may affect survival, fecundity, and the capacity support pathogen development. Here we assess effects carbohydrate sources on susceptibility Plasmodium vivax infection colonies Anopheles darlingi deaneorum mosquitoes. Newly emerged females from each species were fed either 10%...
Despite advances in malaria chemotherapy, the disease continues to claim thousands of lives annually. Addressing this issue requires discovery new compounds counteract resistance threatening current therapeutic arsenal. In context, bi-triazoles are substances with diverse biological activities, showing promise as lead compound fight malaria. Triazoles heterocyclic structures composed five members, including three nitrogen atoms and two double bonds. Bi-triazoles, focus study, derivatives...
The objective of this paper was to describe the current status Mansonella ozzardi prevalence among inhabitants 12 communities along Ituxi river, in Lábrea municipality, state Amazonas. M. determined using thick blood smears obtained by digital punctures. found 30.23% samples collected (39/129), with similar between genders (males: 30.30%; females: 30.16%); highest homemakers (45.45%) followed farmers (38.77%). Among age groups, males and females older than 48 exhibited rates. These results...
This study investigated some epidemiological aspects of the Mansonella ozzardi in municipality Coari, Amazonas. Clinical symptoms were correlated with filarial infection and parasitic rates (PIR) estimated simuliid vectors. The general M. human prevalence rate was 13.3% (231/1733), which 10.2% (109/1069) from urban area 18.4% (122/664) rural area. higher men (14.5% 19.7% rural) than women (6.7% 17.2% occurred most age groups. indices microfilaremics people > or = 51 years old (26.9% 61.5%...
Mansonella ozzardi is a poorly understood human filarial parasite with broad distribution throughout Latin America. Most of what known about its parasitism has come from epidemiological studies that have estimated incidence using light microscopy. Light microscopy can, however, miss lighter, submicroscopic, infections. In this study we compared M. estimates made microscopy, PCR. 214 DNA extracts Large Volume Venous Blood Samples (LVVBS) were taken volunteers two sites in the Rio Solimões...