A5089428248- Parasitic Diseases Research and Treatment
- Parasite Biology and Host Interactions
- Insect symbiosis and bacterial influences
- Parasites and Host Interactions
- Mosquito-borne diseases and control
- Indigenous Health and Education
- Insects and Parasite Interactions
- Trypanosoma species research and implications
- Zoonotic diseases and public health
- Parasitic infections in humans and animals
- Molecular Biology Techniques and Applications
- Travel-related health issues
- Studies on Chitinases and Chitosanases
- Prenatal Screening and Diagnostics
- Public Health and Social Inequalities
- Microbial Metabolism and Applications
- Vector-borne infectious diseases
- Aquatic Invertebrate Ecology and Behavior
- Oral microbiology and periodontitis research
- Healthcare Regulation
- Pharmaceutical and Antibiotic Environmental Impacts
- Malaria Research and Control
- Blood donation and transfusion practices
- Helminth infection and control
- Environmental DNA in Biodiversity Studies
Fundação Oswaldo Cruz
2015-2024
Amazon (United States)
2016-2024
Leoni (Germany)
2019-2021
London School of Hygiene & Tropical Medicine
2009-2013
University of London
2009-2011
Queen Mary University of London
2005
Venezuela's tumbling economy and authoritarian rule have precipitated an unprecedented humanitarian crisis. Hyperinflation rates now exceed 45,000%, health system is in free fall. The country experiencing a massive exodus of biomedical scientists qualified healthcare professionals. Reemergence arthropod-borne vaccine-preventable diseases has sparked serious epidemics that also affect neighboring countries. In this article, we discuss the ongoing measles diphtheria Venezuela their...
Multi-drug resistant forms of Pseudomonas aeruginosa (MDRPA) are a major source nosocomial infections and when discharged into streams rivers from hospital wastewater treatment plants (HWWTP) they known to be able persist for extended periods. In the city Manaus (Western Brazilian Amazon), effluent three HWWTPs feed urban Mindu stream which crosses its rainforest before draining Rio Negro. The is routinely used by residents bathing cleaning (of clothes as well domestic utensils) and, during...
As the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) pandemic continues to expand, healthcare resources globally have been spread thin. Now, disease is rapidly spreading across South America, with deadly consequences in areas already weakened public health systems. The Amazon region particularly susceptible widespread devastation from 2019 (COVID-19) because of its immunologically fragile native Amerindian inhabitants and epidemiologic vulnerabilities. Herein, we discuss...
In this paper we investigate the utility of COI DNA barcoding region for species identification and revealing hidden diversity within subgenus Trichodagmia related taxa in New World. total, 24 morphospecies current expanded taxonomic concept were analyzed. Three Aspathia 10 Simulium s.str. also included analysis because their putative phylogenetic relationship with Trichodagmia. Neighbour Joining tree (NJ) derived from barcodes most specimens grouped together according to or groups as...
We obtained ribosomal and mitochondrial DNA sequences from residents of Amazonas state, Brazil, with Mansonella parasitemias. Phylogenetic analysis these confirm that M. ozzardi perstans parasites occur in sympatry reveal the close relationship between Africa providing insights into parasite's New World origins.
Despite the broad distribution of M. ozzardi in Latin America and Caribbean, there is still very little DNA sequence data available to study this neglected parasite's epidemiology. Mitochondrial (mtDNA) sequences, especially cytochrome oxidase (CO1) gene's barcoding region, have been targeted successfully for filarial diagnostics epidemiological, ecological evolutionary studies. MtDNA-based studies can, however, be compromised by unrecognised mitochondrial pseudogenes, such as Numts. Here,...
Abstract Background Simulium damnosum s.l., the most important vector of onchocerciasis in Africa, is a complex sibling species that have been described on basis differences their larval polytene chromosomes. These (cyto) differ geographical distributions, ecologies and epidemiological roles. In Ghana, distributional changes recorded as consequence control environmental change (e.g. deforestation), with potential disease consequences. We review distribution cytospecies southern Ghana report...
Mansonella ozzardi is a poorly understood human filarial parasite with broad distribution throughout Latin America. Most of what known about its parasitism has come from epidemiological studies that have estimated incidence using light microscopy. Light microscopy can, however, miss lighter, submicroscopic, infections. In this study we compared M. estimates made microscopy, PCR. 214 DNA extracts Large Volume Venous Blood Samples (LVVBS) were taken volunteers two sites in the Rio Solimões...
In a recent issue of Memórias do Instituto Oswaldo Cruz, published in Rio de Janeiro February 2014 (109: 87-92), Adami et al. have survey reporting Mansonella parasite prevalence the Amazon Region. This report makes useful contribution to existing knowledge filarial distribution within area, rates relation age and occupation provides observations on possible clinical impact ozzardi. Their publication also an account what appears be novel ELISA that has recently been used Simuliidae...
Mansonella ozzardi and perstans infections both cause mansonellosis but are usually treated differently. Using a real-time polymerase chain reaction assay deep sequencing, we reveal the presence of coinfections that were undetectable by standard diagnostic methods. Our results confirm have important implications for disease's treatment diagnosis.
ABSTRACT The primates that inhabit the rainforest surrounding city of Manaus (Amazonas, Brazil) have long been recognised as potentially important reservoirs emerging and re-emerging infectious diseases (ERIDs). PCR amplification filarial sequences from wild-caught Simulium oyapockense has used to incriminate Amazon-region ERID bridge vectors by showing they had previously fed on non-human primates. broader use parasite for incrimination biting insects zoonotic disease is limited a paucity...
Abstract Background Immunoassays for Plasmodium detection are, presently, most frequently based on monoclonal antibodies (MAbs); Polyclonal (PAbs), which are cheaper to develop and manufacture, much less used. In the present study we describe a sandwich ELISA assay is capable of detecting P. vivax Lactate Dehydrogenase (LDH) in clinical blood samples, without cross reacting with those infected falciparum . Methods Two recombinant proteins were produced from different regions LDH gene. then...
Vector-biting behaviour is important for vector-borne disease (VBD) epidemiology. The proportion of blood meals taken on humans (the human index, HBI), a component the biting rate per vector in VBD transmission models. Humans are definitive host Onchocerca volvulus, but simuliid vectors feed range animals and HBI key indicator potential onchocerciasis transmission. Ghana has diversity Simulium damnosum complex members, which likely to vary their HBIs, an consideration parameterization...
Abstract. The mermithid parasite, Isomermis lairdi Mondet, Poinar & Bernadou (Nematoda: Mermithidae), is known to have a major impact on populations of Simulium damnosum s.l . Theobald (Diptera: Simuliidae) and their efficiency as vectors Onchocerca volvulus (Leuckart) Filarioidea). However, the value I. other parasites potential means integrated vector control has not been fully realized. This partly because traditional taxonomic approaches insufficient for describing analysing...
We report here the first complete mitochondria genome of Onchocerca volvulus from a focus outside Africa. An O. mitogenome Brazilian Amazonia was obtained using combination high-throughput and Sanger sequencing technologies. Comparisons made between this mitochondrial publicly available sequences identified 46 variant nucleotide positions suggested that our is more closely related to Cameroon-origin than West African-origin mitochondria. As well as providing insights into origins Latin...
Four species of the Mansonella genus infect millions people across sub-Saharan Africa and Central South America. Most infections are asymptomatic, but mansonellosis can be associated with nonspecific clinical manifestations such as fever, headache, arthralgia, ocular lesions (M. ozzardi); pruritus, abdominal pain, angioedema, skin rash, fatigue perstans perhaps sp. 'DEUX'); pruritic dermatitis chronic lymphadenitis perstans).We searched PubMed SciELO databases for publications on...
The population biology of internal parasites is difficult to study because the adult are often inaccessible, deep within host's body. Developing stages, such as eggs in faeces or larvae skin more easily obtained, but handle they very small and with a tough cuticle. This has limited their use molecular ecology for estimating parameters adults (their parents). We have used Onchocerca ochengi (a filarial nematode parasite cattle) describe novel generally applicable method conveniently isolating...