A5000209559- Mosquito-borne diseases and control
- Malaria Research and Control
- Bacteriophages and microbial interactions
- Aquaculture disease management and microbiota
- Biosensors and Analytical Detection
- vaccines and immunoinformatics approaches
- Monoclonal and Polyclonal Antibodies Research
- Advanced Biosensing Techniques and Applications
- Vector-borne infectious diseases
- Enzyme Production and Characterization
- Advanced Cellulose Research Studies
- Microbial infections and disease research
- Advanced biosensing and bioanalysis techniques
- Antibiotic Resistance in Bacteria
- Science and Education Research
- Viral gastroenteritis research and epidemiology
- Invertebrate Immune Response Mechanisms
- Escherichia coli research studies
- Clostridium difficile and Clostridium perfringens research
- Bacterial Genetics and Biotechnology
- Aquaculture Nutrition and Growth
- Characterization and Applications of Magnetic Nanoparticles
- Complement system in diseases
- Electrochemical sensors and biosensors
- HIV Research and Treatment
Universidade Federal do Amazonas
2010-2025
Fundação Oswaldo Cruz
2014-2025
Protein Express (United States)
2024
Amazon (United States)
2023
Leoni (Germany)
2014-2022
Faculdades Oswaldo Cruz
2020
Malaria is a parasitic disease of great relevance in global public health. The development new sensitive and specific diagnostic high-throughput methods remains challenge the eradication this disease. In study, we developed flow cytometry test using latex microbeads polyclonal antibodies obtained from rabbits mice for detection P. vivax lactate dehydrogenase (PvLDH) antigen. We processed 50 samples Brazilian patients diagnosed with malaria caused by 40 healthy individuals. assay presented...
Abstract Background Plasmodium vivax has the potential to infect 2.85 billion individuals worldwide. Nevertheless, limited number of studies investigating immune status living in malaria-endemic areas, as well lack reports serological markers associated with clinical protection, hampered development vaccines for P. . It was previously demonstrated that naturally total IgG against N-terminus merozoite surface protein 1 (Pv-MSP1) reduced risk malarial infection. Methods Immune response Pv-MSP1...
One-step enzyme-free dual electrochemical immunosensor for Malaria biomarker determination.
Abstract Background Immunoassays for Plasmodium detection are, presently, most frequently based on monoclonal antibodies (MAbs); Polyclonal (PAbs), which are cheaper to develop and manufacture, much less used. In the present study we describe a sandwich ELISA assay is capable of detecting P. vivax Lactate Dehydrogenase (LDH) in clinical blood samples, without cross reacting with those infected falciparum . Methods Two recombinant proteins were produced from different regions LDH gene. then...
Abstract Malaria remains a widespread public health problem in tropical and subtropical regions around the world, there is still no vaccine available for full protection. In recent years, it has been observed that spores of Bacillus subtillis can act as carrier adjuvant, promoting an elevated humoral response after co-administration with antigens either coupled or integrated to their surface. our study, B. from KO7 strain were used couple recombinant CSP protein P. falciparum (r Pf CSP),...
Abstract Nano-emulsions are promising carriers for antigen delivery. Here, we evaluated the efficacy of a water-oil nano-emulsion containing concentrated, inactivated Clostridium novyi (C. novyi) type B supernatant culture (nano-iCnB) in protecting Swiss mice against lethal dose alpha toxin concentrated extract. Proteins were confirmed nano-iCnB and their stabilities determined according physical parameters such as Zeta Potential (ZP). Biochemical, hematological morphological appearance...
Since their discovery, carbon nanotubes were used for numerous applications in the most diverse knowledge areas. However, lack of solubility these molecules aqueous media compromises beneficial properties certain applications. Several methods to solubilize are described, however, depending on intended application, impact that solubilization has physical and chemical needs be considered. In present study, a simple methodology is described utilizes polyvinylpyrrolidone combined with sonication...
Summary Previous studies have implicated reactive antibodies to the low molecular weight rhoptry‐associated proteins (RAP‐1, RAP‐2/RSP‐2 and RAP‐3) in erythroid cell destruction during Plasmodium falciparum infection. In this pilot study, frequency, specificity functional capacity of naturally acquired anti‐RAP‐2/RSP‐2 were investigated sera anaemic nonanaemic malaria‐infected Cameroonian children. All recognized by FACS, irrespective clinical status subjects. However, children showed higher...
Anaplasma marginale (A. marginale) has a remarkable impact on livestock production, and an effective vaccine is not currently available due to the inexistence of small animal model. Recently, BALB/c mice were successfully infected with A. marginale, resulting in acute persistent anaplasmosis infection. Here, we designed hybrid protein containing repeats polypeptide 1a from major surface protein-1 complex (MSP1a) common epitopes outer membrane proteins (OMPs) OMP7, OMP8 OMP9 expressed...
Malaria is a major public health challenge worldwide and requires accurate efficient diagnostic methods. Traditional approaches based on antigen-antibody interactions are associated with ethical economic concerns. Molecularly imprinted polymers (MIPs) offer promising alternative by providing complementary polymer structure capable of selectively binding target molecules. In this study, we developed liquid, redox-probe-free, MIP-based electrochemical biosensor to detect the
The diversity of MSP1 in both Plasmodium falciparum and P. vivax is presumed be associated to parasite immune evasion. In this study, we assessed genetic the most variable domain vaccine candidate N-terminal PvMSP1 (Block 2) field isolates Manaus. Forty-seven blood samples polymorphism Block 2 generates four fragment sizes. twenty-eight them, sequencing indicated seven haplotypes circulating among isolates. Evidence striking exchanges was observed with two stretches flanking repeat region...
Magnetic nanoparticles (NPs) have attracted great attention owing to their applications in the biomedical field.In present work, maghemite (γFe 2 O 3 ) NPs of 6.5 nm were prepared using a sonochemical method and used prepare magnetic beads through silanization with 3-aminopropyltrimethoxysilane (APTS).Subsequently, amino groups resulting APTS-γFe converted carboxylic acid (CARB-γFe succinic anhydride reaction, as confirmed by transmission electron microscopy (TEM), Fourier transform infrared...
During the period from 2000 to 2002, 79 rotavirus-positive stool samples were collected children presenting diarrhea in Western Brazilian Amazon.Molecular characterization of G and P genotypes was performed using RT-PCR electropherotyping analysis by polyacrylamide gel electrophoresis.A total 59 confirmed as group A rotavirus. long electrophoretic profile exhibited G1P[8], G3P[8], G4P[8] genotypes. The G1P[8] genotype found greater proportion. short electropherotype only G2 strains.The...
Abstract Hepatitis C is a public health problem worldwide, affecting chronically about 71 million people, or 1% of the world population. The early detection infection in clinical practice routine and blood donors still important, being necessary for development new specific, sensitive, reliable tests that can improve screening HCV infection. Flow cytometry beads assay opens possibility to detect multiple antigens, decrease cross-reactivity, increase sensitivity, with low sample volumes....