ABSTRACT Bacillus subtilis spores are being used for oral bacteriotherapy and bacterioprophylaxis of gastrointestinal disorders in both humans animals. Since B. is an aerobic saprophyte, how may benefit the gut microbiota intriguing question, since other probiotics such as Lactobacillus spp. which colonize anerobes. As a first step understanding potential effects ingesting spores, we have characterized five commercial products. An extensive biochemical, physiological, phylogenetic analysis...

ABSTRACT We developed a novel surface display system based on the use of bacterial spores. A protein Bacillus subtilis spore coat, CotB, was found to be located and used as fusion partner express 459-amino-acid C-terminal fragment tetanus toxin (TTFC). Western, dot blot fluorescent-activated cell sorting analyses were monitor TTFC expression purified estimated that more than 1.5 × 10 3 molecules exposed each recognized by TTFC-specific antibodies. The efficient presentation heterologous...

ABSTRACT For the first time, bacterial spores have been evaluated as vaccine vehicles. Bacillus subtilis displaying tetanus toxin fragment C (TTFC) antigen were used for oral and intranasal immunization shown to generate mucosal systemic responses in a murine model. TTFC-specific immunoglobulin G titers serum (determined by enzyme-linked immunosorbent assay) reached significant levels 33 days after dosing, while against spore coat proteins relatively low. Tetanus antitoxin sufficient protect...

A fructose-rich diet can induce metabolic syndrome, a combination of health disorders that increases the risk diabetes and cardiovascular diseases. Diet is also known to alter microbial composition gut, although it not clear whether such alteration contributes development syndrome. The aim this work was assess possible link between gut microbiota diet-induced syndrome in rat model obesity. Rats were fed either standard or high-fructose diet. Groups fructose-fed rats treated with antibiotics...

Probiotics have health-beneficial properties mainly due to either a direct action on the host or modulation of microbiota. Health-beneficial also been associated with variety plant-derived molecules, widely used as dietary supplements. This study explores possibility combining actions probiotics and molecules by developing beneficial, probiotic-carrying, heterologous molecules. To this extent, spores SF174, well-characterized probiotic strain Shouchella clausii (formerly Bacillus clausii),...

Bacterial spores are being consumed as probiotics, although little is known about their efficacy or mode of action. As a first step in characterizing spore we have studied the persistence and dissemination Bacillus subtilis given orally to mice. Our results shown that do not appear disseminate across mucosal surfaces. However, found number excreted feces mice was, some experiments, larger than original inoculum. This was an intriguing result might be explained by germination proportion...

A bacteriocin-producing Bacillus cereus strain was isolated. The bacteriocin, here called cerein, shown to be active specifically against other B. strains and inactive all bacterial species tested. Cerein detected in the culture supernatants of stationary-phase cells, its appearance inhibited by induction sporulation. activity cerein insensitive organic solvents nonproteolytic enzymes, partially stable heat, over a wide range pH values. Direct detection antimicrobial on sodium dodecyl...

Journal Article Carotenoids found in Bacillus Get access R. Khaneja, Khaneja School of Biological Sciences, Royal Holloway, University London, Egham, Surrey, UK Search for other works by this author on: Oxford Academic Google Scholar L. Perez‐Fons, Perez‐Fons Centre Systems and Synthetic Biology, S. Fakhry, Fakhry Department Structural Functional Federico II University, Naples, Italy Baccigalupi, Baccigalupi Steiger, Steiger J.W. Goethe Universität Frankfurt, Germany E. To, To G. Sandmann,...

Producing antimicrobials is a common adaptive behavior shared by many microorganisms, including marine bacteria. We report that SF214, marine-isolated strain of Bacillus pumilus, produces at least two different molecules with antibacterial activity: molecule smaller than 3 kDa active against Staphylococcus aureus and larger 10 Listeria monocytogenes. focused our attention on the anti-Staphylococcus found it was wide range pH conditions its secretion dependent growth phase, medium,...

Bacterial spores are commonly isolated from a variety of different environments, including extreme habitats. Although it is well established that such ubiquitous distribution reflects the spore resistance properties, not clear whether growing conditions affect structure and function. We used Bacillus subtilis similar age but produced at 25, 37, or 42°C to compare their surface structures functional properties. Spores 25°C were more hydrophobic while those contained dipicolinic acid,...

ABSTRACT Bacillus subtilis is currently used as an oral probiotic. We examined two commercial B. probiotic preparations, Enterogermina and Biosubtyl. Surprisingly, physiological genetic characterization of the bacteria contained in each these preparations has shown that neither contains .

Endospores of Bacillus subtilis are encased in a protein shell, known as the spore coat, composed lamella-like inner layer and an electron-dense outer layer. We report identification characterization gene, herein called cotH, located at 300 degrees on B. genetic map between two divergent cot genes, cotB cotG. The cotH open reading frame extended for 1,086 bp corresponded to polypeptide 42.8 kDa. Spores null mutant were normally heat, lysozyme, chloroform resistant but impaired germination....

Sporulating cells of the gram-positive bacterium Bacillus subtilis are partitioned into two cellular compartments called mother cell and forespore. Gene expression in forespore is regulated differentially by compartment-specific transcription factors sigma K G, respectively. between also coordinated a signal transduction pathway that couples activation (by processing its inactive precursor pro-sigma K) to G-directed gene To dissect genetically, we previously isolated bypass mutations at loci...

Summary We report the cloning and characterization of an unusually small gene called spoVM whose product Is required for normal formation cortex coat during sporulation in Bacillus subtilis. The is adjacent to, convergent orientation with, B. subtilis homologue to Escherichia coli ribosomal protein L28. open reading frame only 26 codons length capable encoding a polypeptide 3 kDa. short was verified by means complementation experiments with wild‐type deletion‐mutated copies engineering...

We describe the identification and characterization of a gene, herein designated cotG, encoding an abundant coat protein from spores Bacillus subtilis. The cotG open reading frame is 195 codons in length capable polypeptide 24 kDa that contains nine tandem copies 13-amino-acid long, approximately repeated sequence H/Y-K-K-S-Y-R/C-S/T-H/Y-K-K-S-R-S. located at 300 degrees on genetic map close to another cotB. cotB genes are divergent orientation separated by 1.3 kb. Like promoter for cotB,...

To isolate and characterize spore-former bacteria able to colonize the human gastrointestinal tract (GIT).A total of 25 spore-formers was isolated from faeces ileal biopsies healthy volunteers identified at species level. Physiological analysis performed evaluate ability various isolates form biofilms, swarm, produce surfactants molecules that have antimicrobial activity against selected pathogens. assess potential probiotic isolates, we tested resistance cells spores simulated gastric...

Abstract Background The bacterial endospore (spore) has recently been proposed as a new surface display system. Antigens and enzymes have successfully exposed on the layers of Bacillus subtilis spore, but only in few cases efficiency expression effective determined. We used this heterologous system to produce A subunit urease animal pathogen Helicobater acinonychis . Ureases are multi-subunit with central role virulence various pathogens necessary for colonization gastric mucosa by human H....

Abstract Coeliac disease (CD) is a very common food‐sensitive enteropathy, which triggered by gluten ingestion and mediated CD4 + T cells. In addition, alterations in the intestinal microbiota that normally involved homeostasis of GALT (gut‐associated lymphoid tissue) seem to play role CD. accordance with these findings, we previously reported Lactobacillus casei can induce strong enhancement cell‐mediated response gliadin without inducing enteropathy. this study, analysed effects L....

Litopenaeus vannamei do not have an adaptive immune response system. White spot syndrome virus (WSSV) is the most severe pathogen in shrimps. Bacillus subtilis spores carrying heterologous antigens on its surface been evaluated as a vaccine inducing specific systemic responses vertebrates. Orally administrated Vp28 vaccines investigated crustaceans. Vp26 also important constituent of WSSV structure but little known about oral vaccination capacity L. vannamei.In this study, for first time,...