A5103173458- Bacterial Genetics and Biotechnology
- Bacteriophages and microbial interactions
- Microbial Metabolic Engineering and Bioproduction
- Enzyme Catalysis and Immobilization
- Microbial bioremediation and biosurfactants
- Molecular Sensors and Ion Detection
- Essential Oils and Antimicrobial Activity
- Advanced biosensing and bioanalysis techniques
- Microbial Community Ecology and Physiology
- Genomics and Phylogenetic Studies
- Metal-Catalyzed Oxygenation Mechanisms
- Bacterial Infections and Vaccines
- Enzyme Structure and Function
- Bioactive Natural Diterpenoids Research
- Biochemical and biochemical processes
- Steroid Chemistry and Biochemistry
- Microbial metabolism and enzyme function
- Plant chemical constituents analysis
- Insect Utilization and Effects
- bioluminescence and chemiluminescence research
- Cystic Fibrosis Research Advances
- Biochemical and Structural Characterization
- Cell death mechanisms and regulation
- Mass Spectrometry Techniques and Applications
- DNA and Nucleic Acid Chemistry
University of Salerno
2020-2024
University of Naples Federico II
2015-2024
Federico II University Hospital
2016-2022
Ospedali Riuniti San Giovanni di Dio e Ruggi d'Aragona
2021
University of Exeter
2015-2018
Naples Anesthesia & Physician Associates
2016
Ceinge Biotecnologie Avanzate (Italy)
2011
Gram-negative bacteria release Outer Membrane Vesicles (OMVs) into the extracellular environment. Recent studies recognized these vesicles as vectors to horizontal gene transfer; however, parameters that mediate OMVs transfer within bacterial communities remain unclear. The present study highlights for first time of plasmids containing resistance genes via derived from Klebsiella pneumoniae (K. pneumoniae). This mechanism confers DNA protection, it is plasmid copy number dependent with a...
Bacterial spores are commonly isolated from a variety of different environments, including extreme habitats. Although it is well established that such ubiquitous distribution reflects the spore resistance properties, not clear whether growing conditions affect structure and function. We used Bacillus subtilis similar age but produced at 25, 37, or 42°C to compare their surface structures functional properties. Spores 25°C were more hydrophobic while those contained dipicolinic acid,...
A novel metal ion-sensitive fluorescent peptidyl-probe has been designed based on the most common five-residue repeat in mammalian histidine rich glycoproteins (HRGs). dansyl-amide moiety at N-terminus and a tryptophan residue C-terminus of peptide were added as they can act FRET (fluorescence resonance energy transfer) pair. The dansyl fluorophore was chosen also because it frequently shows strong CHEF (chelation enhanced fluorescence) solvatochromic effects. peptide, dansyl-HPHGHW-NH2...
Abstract Endogenous reactive oxygen species (ROS) are by-products of the aerobic metabolism cells and have an important signalling role as secondary messengers in various physiological processes, including cell growth development. However, excessive production ROS, well exposure to exogenous can cause protein oxidation, lipid peroxidation DNA damages leading injuries. ROS accumulation has been associated development health disorders such neurodegenerative cardiovascular diseases,...
Inorganic trivalent arsenic is a major environmental pollutant and exposure to human results in many pathologies, including keratosis carcinoma. Here, we analyzed the effects of B. subtilis spores on normal keratinocytes presence sodium arsenite oxidative stress. Pre-treatment cells with before inducing stress was able keep levels intracellular ROS, GSH lipid peroxidation, as well inhibit activation MAPK cascade. Moreover, showed positive effect cell proliferation, probably due their binding...
Abstract Gram-negative bacteria release outer membrane vesicles (OMVs) into the extracellular environment. Recent studies recognized these as vectors to horizontal gene transfer, however parameters that mediate OMVs transfer within bacterial communities remain unclear. The present study highlights for first time of plasmids containing resistance genes via derived from Klebsiella pneumoniae ( K. ). This mechanism confers DNA protection and it is plasmid copy number dependent with a ratio 3.6...
Proteins and glycoproteins that form the surface layers of Bacillus spore assemble into semipermeable arrays surround protect cytoplasm. Such layers, acting like molecular sieves, exclude large molecules but allow small nutrients (germinants) to penetrate. We report CotG, a modular abundant component subtilis coat, controls permeability through its central region, formed by positively charged tandem repeats. These repeats act as spacers between N C termini protein, which are responsible for...
Biocatalysis is today a standard technology for the industrial production of several chemicals, and number biotransformation processes running on commercial scale constantly increasing. Among biocatalysts, bacterial multicomponent monooxygenases (BMMs), diverse group nonheme diiron enzymes that activate dioxygen, are primary interest due to their ability catalyze variety complex oxidations, including reactions mono- dihydroxylation phenolic compounds. In recent years, both directed evolution...
Bacterial spores have been proposed as vehicles to display heterologous proteins for the development of mucosal vaccines, biocatalysts, bioremediation and diagnostic tools. Two approaches developed on spore surface: a recombinant approach, based construction gene fusions between DNA molecules coding surface protein (carrier) be displayed (passenger); non-recombinant approach adsorption, spontaneous interaction negatively charged, hydrophobic purified proteins. The molecular details...
The three-dimensional structures of the native enzyme and FMN complex overexpressed form oxygenating component type II Baeyer-Villiger 3,6-diketocamphane monooxygenase have been determined to 1.9 Å resolution. structure this dimeric FMN-dependent enzyme, which is encoded on large CAM plasmid Pseudomonas putida, has solved by a combination multiple anomalous dispersion from bromine crystal soak molecular replacement using bacterial luciferase model. orientation isoalloxazine ring cofactor in...
Bacterial spores spontaneously interact and tightly bind heterologous proteins. A variety of antigens enzymes have been efficiently displayed on Bacillus subtilis, the model system for spore formers. Adsorption B. subtilis has then proposed as a non-recombinant approach development mucosal vaccine/drug delivery vehicles, biocatalysts, bioremediation diagnostic tools. We used megaterium QM B1551 to evaluate their efficiency an adsorption platform. Spores are significantly larger than those...
The development of efficient mucosal vaccines is strongly dependent on the use appropriate vectors. Various biological systems or synthetic nanoparticles have been proposed to display and deliver antigens surfaces. Bacillus spore, a metabolically quiescent extremely resistant cell, has also as vaccine delivery system shown able conjugate advantages live systems. Several displayed spore by either recombinant non-recombinant approaches, antigen-specific immune responses observed in animals...
Monocyclic phenols and catechols are important antioxidant compounds for the food pharmaceutic industries; their production through biotransformation of low-added value starting is major biotechnological interest. The toluene o-xylene monooxygenase (ToMO) from Pseudomonas sp. OX1 a bacterial multicomponent (BMM) that able to hydroxylate wide array aromatic has already proven be versatile biochemical tool produce mono- dihydroxylated derivatives compounds. molecular determinants its...
Heavy metals are hazardous environmental contaminants, often highly toxic even at extremely low concentrations. Monitoring their presence in samples is an important but complex task that has attracted the attention of many research groups. We have previously developed a fluorescent peptidyl sensor, dH3w, for monitoring Zn2+ living cells. This probe, designed on base internal repeats human histidine rich glycoprotein, shows turn response to and off Cu2+. Other heavy (Mn2+, Fe2+, Ni2+, Co2+,...
The ent-kaurane diterpene oridonin was reported to inhibit cell migration and invasion in several experimental models. However, the process by which this molecule exerts its anti-metastatic action has not been yet elucidated. In article, we have investigated activity of Oridonin one homolog, Irudonin, with aim shed light on molecular mechanisms underlying biological these diterpenes. Cell-based experiments revealed that both compounds are able affect differentiation cytoskeleton organization...
Arthrospira platensis (Spirulina) has been credited with multiple beneficial effects, many of which are attributed to bioactive peptides produced during the gastrointestinal digestion this micro-alga. Many Spirulina-based nutraceuticals have produced, and numerous functional foods enriched Spirulina available on market. These subjected checks aimed at verifying amount algae actually present, but few studies relating bioavailability compounds in these products carried out. However, such...
Carbon nanotubes (CNTs) are among the most versatile nanomaterials, but their exploitation is hindered by limited dispersibility, especially in aqueous solvents. Here, we show that AP-LYS, a highly cationic soluble derivative of denatured hen egg lysozyme, very effective tool for unbundling and solubilisation CNTs. AP-LYS proved to mediate complete stable dispersion CNTs at protein: CNT ratios ≥1: 3 (w:w) mild conditions (10-20 minutes sonication ammonium acetate buffer, pH 5.0)....
Previously, we reported that fluorescent peptide dansyl-HPHGHW-NH
Premna, a genus consisting of approximately 200 species, predominantly thrives in tropical and subtropical areas. Many these species have been utilized ethnopharmacology for diverse medicinal applications. In Saudi Arabia, Premna resinosa (Hochst.) Schauer (Lamiaceae) grows wildly, its slightly viscid leaves are attributed to the production leaf accession. this study, we aimed extract surface accession from fresh using dichloromethane evaluate anticancer potential. The plant exudate yielded...
Abstract Efficient predictive biomarkers are needed for immune checkpoint inhibitor (ICI)-based immunotherapy in non-small-cell lung cancer (NSCLC). So far, testing the value of single nucleotide polymorphisms (SNPs) programmed cell death 1 (PD-1) or its ligand (PD-L1) has shown contrasting results. Here, we aimed to validate PD-L1 SNPs advanced NSCLC patients treated with ICIs as well define molecular mechanisms underlying role identified SNP candidate. rs822336 efficiently predicted...