A5001151520- Soybean genetics and cultivation
- Glycosylation and Glycoproteins Research
- Plant pathogens and resistance mechanisms
- Phytoestrogen effects and research
- Lysosomal Storage Disorders Research
- Photosynthetic Processes and Mechanisms
- Carbohydrate Chemistry and Synthesis
- Legume Nitrogen Fixing Symbiosis
- Polysaccharides and Plant Cell Walls
- Banana Cultivation and Research
- RNA Research and Splicing
- Cellular Mechanics and Interactions
- RNA and protein synthesis mechanisms
- Plant Gene Expression Analysis
- Ginseng Biological Effects and Applications
- Plant tissue culture and regeneration
- Natural product bioactivities and synthesis
- Pancreatic function and diabetes
- Cassava research and cyanide
- Plant nutrient uptake and metabolism
- Plant Genetic and Mutation Studies
- Aluminum toxicity and tolerance in plants and animals
- Proteoglycans and glycosaminoglycans research
- Metabolism, Diabetes, and Cancer
- Plant-Microbe Interactions and Immunity
National Research Centre for Banana
2021-2022
Institute of Crop Science
2019-2020
National Institute of Agricultural Science and Technology
2016-2019
Chonnam National University
2012-2016
Institute of Agrobiological Sciences
2016
National Academy of Agricultural Science
2014-2015
Sanford Burnham Prebys Medical Discovery Institute
1995-2000
National Foundation for Cancer Research
1995-1996
Type I carbohydrate-deficient glycoprotein syndrome (CDGS) patients fail to add entire N-linked oligosaccharide chains some serum glycoproteins. Here we show that four CDGS fibroblast cell lines have two related glycosylation abnormalities. First, they incorporate 3-10-fold less [3H] mannose into proteins, and, second, the size of lipid-linked precursor (LLO) is much smaller than in controls. Addition exogenous mannose, but not glucose, these cells corrects both lowered incorporation and...
The concentration of D-mannose in serum is 20-50 micron, but its physiological significance for glycoprotein synthesis unknown. Here, we show that the uptake by different mammalian cell lines involves a mannose-specific transporter(s) with K(uptake) about 30-70 micron and V(max) which probably sufficient to account bulk mannose needed synthesis. Mannose appears be through facilitated transport process since it not inhibited cyanide. Phloretin completely inhibits uptake, phloridzin only...
Mannose in N-linked oligosaccharides is assumed to be derived primarily from glucose through phosphomannose isomerase (PMI). The discovery of mammalian mannose-specific transporters that function at physiological concentrations suggested mannose might directly contribute oligosaccharide synthesis. To determine the relative contribution and mannose, human fibroblasts were labeled with either [2-3H]mannose or [1,5,6-3H]glucose same specific activity, theN-linked chains released by PNGase F...
Congenital Disorders of Glycosylation (CDG) are human deficiencies in glycoprotein biosynthesis. Previous studies showed that 1 mM mannose corrects defective protein N-glycosylation cultured fibroblasts from some CDG patients. We hypothesized these cells have limited GDP-mannose (GDP-Man) and exogenous increases the GDP-Man levels. Using a well established method to measure GDP-Man, we found normal had an average 23.5 pmol GDP-Man/106 cells, whereas phosphomannomutase (PMM)-deficient only...
Multidrug and toxic compound extrusion (MATE) transporters comprise a multigene family that mediates multiple functions in plants through the efflux of diverse substrates including organic molecules, specialized metabolites, hormones, xenobiotics. MATE classification based on genome-wide studies remains ambiguous, likely due to lack large-scale phylogenomic and/or reference sequence datasets. To resolve this, we established phylogeny plant gene using comprehensive kingdom-wide analysis 74...
Abstract Seed saponin composition of 3025 wild soybean ( G lycine soja S ieb. and Z ucc.) accessions collected from nine regions K orea was analysed by thin‐layer chromatography to determine its polymorphic variation geographical distribution find mutants in components. The seed hypocotyls primarily divided into seven phenotypes, designated as Aa, Ab, AaBc, AbBc, Aa+α, AaBc+α AbBc+α. predominant phenotypes were AaBc (55%), Aa (33%), (7.5%) Aa+α (3.3%). frequencies AbBc AbBc+α very low...
The functional role of expansin-like A and B family members remains unclear in plants. In this study, we investigate the Brassica rapa B1 (BrEXLB1) by overexpressing BrEXLB1 sense, antisense specific promoter: GUS Arabidopsis. overexpressors sense BrEXLB1, showed variable unstable pleiotropic effects on leaf growth. Interestingly, enhances plant height than wild types. reporter-aided analysis promoter their activity prominently seeds, roots carpels. This is further confirmed relative...
Abstract Kunitz trypsin inhibitor ( KT i) in 1368 accessions of wild soybean Glycine soja Sieb. & Zucc.), collected from three regions Korea, was examined for allelic diversity and geographical distribution. Five electrophoretically distinguishable i forms were detected: common Tia , Tib Tia/Tib ) two previously unreported i7‐1 i5 ). The allele predominant (93.49%). Alleles detected with the frequencies 3.47, 0.55 0.11%, respectively. heterozygous form frequency 2.26%. nucleotide...
Triterpenes (C30) constitute one of the diverse class natural products with potential applications in food, cosmetic and pharmaceutical industries. Soyasaponins are oleanane-type triterpenoids widespread among legumes particularly abundant soybean seeds. They have associated various pharmacological implications undesirable taste properties soybean-based food products. Uncovering biosynthetic genes soyasaponins will provide new opportunities to control pathway for human benefits. However,...
Eight wild soybean accessions with different saponin phenotypes were used to examine composition and relative quantity in various tissues of mature seeds two-week-old seedlings by LC-PDA/MS/MS. Saponin content varied according accessions. The average total concentration 1 g dry was 16.08 ± 3.13 μmol. In seedlings, produced from seeds, it 27.94 6.52 Group A saponins highly concentrated seed hypocotyl (4.04 0.71 μmol). High DDMP (7.37 5.22 μmol) Sg-6 (2.19 0.59 found cotyledonary leaf. the...
Plant uridine 5'-diphosphate glycosyltransferases (UGTs) influence the physiochemical properties of several classes specialized metabolites including triterpenoids via glycosylation. To uncover evolutionary past UGTs soyasaponins (a group beneficial triterpene glycosides widespread among Leguminosae), UGT gene superfamily in Medicago truncatula, Glycine max, Phaseolus vulgaris, Lotus japonicus, and Trifolium pratense genomes were systematically mined. A total 834 nonredundant identified...
Abstract Group A acetylsaponins are the main causative components for bitter and astringent tastes of soybean ( Glycine max ). In this study, we examined genetic nature absence group in 12 Korean wild soja ) accessions. all accessions, coding region (1431‐bp) Sg‐1 locus was identical with a , which adds xylose sugar moiety at terminal position C‐22 chain SS ‐A, except one nucleotide (G→A change) +948th position. This point mutation results change amino acid from tryptophan TGG to stop codon...
ABSTRACT Saponins are major components of secondary metabolites in soybean [ Glycine max (L.) Merr.] seed that have been broadly studied for their beneficial effects on human health. In soybean, group A acetylsaponins can be classified into two types: Aa‐ and Ab‐series, both which cause bitter astringent aftertaste. Therefore, acetylsaponin‐deficient cultivars solve a problem has inhibited the broader use food industry. The objective this study was to investigate mutant (PE1515) generated...