A5101727401- Plant Stress Responses and Tolerance
- Plant Molecular Biology Research
- Plant Gene Expression Analysis
- Plant nutrient uptake and metabolism
- Phytoplasmas and Hemiptera pathogens
- Photosynthetic Processes and Mechanisms
- Soybean genetics and cultivation
- Plant responses to water stress
- Plant Pathogenic Bacteria Studies
- Cocoa and Sweet Potato Agronomy
- Plant Genetic and Mutation Studies
- Plant tissue culture and regeneration
- Amino Acid Enzymes and Metabolism
- Enzyme Structure and Function
- Light effects on plants
- Plant Virus Research Studies
- Seed Germination and Physiology
- Metabolism and Genetic Disorders
- Rice Cultivation and Yield Improvement
- Plant Reproductive Biology
- Enzyme function and inhibition
- GABA and Rice Research
- Polyamine Metabolism and Applications
- Cancer, Hypoxia, and Metabolism
- Plant Pathogens and Fungal Diseases
Japan International Research Center for Agricultural Sciences
2014-2024
The University of Tokyo
2009-2013
Nagasaki University
1988-2009
Tottori University
2000-2004
RIKEN
2004
Mie University
1986-2000
University of California, Berkeley
2000
Ministry of Agriculture, Forestry and Fisheries
1990-1999
Kyoto Pharmaceutical University
1999
Asahi University
1997
The MYC-like sequence CATGTG plays an important role in the dehydration-inducible expression of Arabidopsis thaliana EARLY RESPONSIVE TO DEHYDRATION STRESS 1 (ERD1) gene, which encodes a ClpA (ATP binding subunit caseinolytic ATP-dependent protease) homologous protein. Using yeast one-hybrid system, we isolated three cDNA clones encoding proteins that bind to 63-bp promoter region erd1, contains motif. These encode named ANAC019, ANAC055, and ANAC072, belong NAC transcription factor family....
Various abiotic stresses such as drought, high salinity, temperature, and low temperature negatively impact plant growth productivity of crops.Plants have adapted to respond these at the molecular, cellular, physiological, biochemical level, enabling them survive.Various adverse environmental induce expression a variety genes in many species (Xiong et al., 2002;Shinozaki 2003; Bartels Sunkar, 2005).Numerous stress-induced been identified using microarray experiments (Kreps 2002;Seki...
Summary The OsNAC6 gene is a member of the NAC transcription factor family in rice. Expression induced by abiotic stresses, including cold, drought and high salinity. expression also wounding blast disease. A transactivation assay using yeast system demonstrated that functions as transcriptional activator, transient localization studies with OsNAC6–sGFP fusion protein revealed its nuclear localization. Transgenic rice plants over‐expressing constitutively exhibited growth retardation low...
Summary Many abiotic stress‐inducible genes contain two cis ‐acting elements, namely a dehydration‐responsive element (DRE; TACCGACAT) and an ABA‐responsive (ABRE; ACGTGG/TC), in their promoter regions. We precisely analyzed the 120 bp region (−174 to −55) of Arabidopsis rd29A gene whose expression is induced by dehydration, high‐salinity, low‐temperature, abscisic acid (ABA) treatments contains DRE, DRE/CRT‐core motif (A/GCCGAC), ABRE sequences. Deletion base substitution analyses this...
ABA is an important phytohormone regulating various plant processes, including stress tolerance, seed development and germination. SRK2D/SnRK2.2, SRK2E/SnRK2.6/OST1 SRK2I/SnRK2.3 are redundant ABA-activated SNF1-related protein kinases 2 (SnRK2s) in Arabidopsis thaliana. We examined the role of these These SnRK2 proteins were mainly expressed nucleus during The triple mutant (srk2d srk2e srk2i) was sensitive to desiccation showed severe growth defects development. It exhibited a loss...
Responses to water stress are thought be mediated by transcriptional regulation of gene expression via reversible protein phosphorylation events. Previously, we reported that bZIP (basic-domain leucine zipper)-type AREB/ABF (ABA-responsive element-binding protein/factor) transcription factors involved in ABA signaling under conditions Arabidopsis. The AREB1 is phosphorylated vitro ABA-activated SNF1-related kinase 2s (SnRK2s) such as SRK2D/SnRK2.2, SRK2E/SnRK2.6 and SRK2I/SnRK2.3...
Summary Many plant genes have been shown to be induced by water stress and function in tolerance. The erd1 gene has upregulated response both etiolation. Promoter studies using the promoter region fused luciferase ( LUC ) reporter Arabidopsis thaliana were performed identify putative cis elements involved. Results indicated that elements, responsible for expression during dehydration etiolation, are separately located two discrete portions of promoter. Base substitution analysis showed a...
In plants, osmotic stress-responsive transcriptional regulation depends mainly on two major classes of cis-acting elements found in the promoter regions stress-inducible genes: ABA-responsive (ABREs) and dehydration-responsive (DREs). ABRE has been shown to perceive ABA-mediated stress signals, whereas DRE is known be involved an ABA-independent pathway. Previously, we reported that transcription factor DRE-BINDING PROTEIN 2A (DREB2A) regulates DRE-mediated target genes under conditions...
Abstract OsTZF1 is a member of the CCCH-type zinc finger gene family in rice (Oryza sativa). Expression was induced by drought, high-salt stress, and hydrogen peroxide. expression also abscisic acid, methyl jasmonate, salicylic acid. Histochemical activity β-glucuronidase transgenic plants containing promoter fused with observed callus, coleoptile, young leaf, panicle tissues. Upon OsTZF1-green fluorescent protein localization cytoplasm cytoplasmic foci. Transgenic overexpressing driven...
Summary Leaf senescence is the terminal phenotype of plant leaf development, and ethylene a major hormone inducing senescence. Recent studies have shown that abscisic acid (ABA) also induces However, detailed mechanisms ABA‐induced remain unclear. We focused on A subfamily stress‐responsive NAC (SNAC‐A) transcription factors, expression which induced by abiotic stresses, particularly ABA. Gene analysis revealed seven SNAC‐A genes including ANAC055 , ANAC019 ANAC072 / RD26 ANAC002 ATAF1...
Many plants, including Arabidopsis thaliana , increase in freezing tolerance response to low non‐freezing temperatures, a phenomenon which is known as cold acclimation. Molecular studies plants have shown that several genes with various functions are induced by temperature (cold stress) and osmotic stress such drought high salinity. For stress‐inducible genes, cis ‐acting elements promoters regions the corresponding transcription factors affect expression of these been analyzed . The...
Summary The ZFHD recognition sequence (ZFHDRS) and NAC (NACRS) play an important role in the dehydration‐inducible expression of Arabidopsis thaliana EARLY RESPONSIVE TO DEHYDRATION STRESS 1 ( ERD1 ) gene. Using yeast one‐hybrid system, we isolated a cDNA encoding ZFHD1 transcriptional activator that specifically binds to 62 bp promoter region , which contains ZFHDRS. Both vitro vivo analyses confirmed specific binding ZFHDRS, was induced by drought, high salinity abscisic acid. DNA‐binding...
In flowering plants, two cells are fertilized in the haploid female gametophyte. Egg and sperm nuclei fuse to form embryo. A second nucleus fuses with central cell nucleus, which replicates generate endosperm, a tissue that supports embryo development. The FERTILIZATION-INDEPENDENT ENDOSPERM (FIE) MEDEA (MEA) genes encode WD SET domain polycomb proteins, respectively. absence of fertilization, gametophyte loss-of-function fie or mea allele initiates endosperm development without...
Abstract Transcription factors of the DRE-Binding1 (DREB1)/C-repeat binding factor family specifically interact with a cis-acting dehydration-responsive element/C-repeat involved in low-temperature stress-responsive gene expression Arabidopsis (Arabidopsis thaliana). Expression DREB1s is induced by low temperatures and regulated circadian clock under unstressed conditions. Promoter sequences contain six conserved motifs, boxes I to VI. We analyzed promoter region DREB1C using transgenic...