A5062759754- Plant Stress Responses and Tolerance
- Plant Molecular Biology Research
- Plant Virus Research Studies
- Plant nutrient uptake and metabolism
- Plant Gene Expression Analysis
- Photosynthetic Processes and Mechanisms
- CRISPR and Genetic Engineering
- Plant and Fungal Interactions Research
- Polyamine Metabolism and Applications
- Transgenic Plants and Applications
- Aluminum toxicity and tolerance in plants and animals
- Insect-Plant Interactions and Control
- Plant Micronutrient Interactions and Effects
- Plant responses to water stress
- Plant-Microbe Interactions and Immunity
- Legume Nitrogen Fixing Symbiosis
- Chromosomal and Genetic Variations
- Genetic Mapping and Diversity in Plants and Animals
- Plant tissue culture and regeneration
- Postharvest Quality and Shelf Life Management
- Mosquito-borne diseases and control
- Atrial Fibrillation Management and Outcomes
- Plant Disease Management Techniques
- Amino Acid Enzymes and Metabolism
- Seed and Plant Biochemistry
Okayama University
2019-2025
RIKEN Center for Sustainable Resource Science
2014-2024
Saiseikai Kumamoto Hospital
2024
The University of Tokyo
2009
Japan International Research Center for Agricultural Sciences
2009
Kazusa DNA Research Institute
2009
RIKEN BioResource Research Center
2009
Gifu University
2009
Japan Science and Technology Agency
2003-2007
Centre de Recherche en Économie et Statistique
2004-2005
Summary Full‐length cDNAs are essential for functional analysis of plant genes in the post‐sequencing era Arabidopsis genome. Recently, cDNA microarray has been developed quantitative global and simultaneous expression profiles. We have prepared a full‐length containing ≈7000 independent, groups to analyse profiles under drought, cold (low temperature) high‐salinity stress conditions over time. The transcripts 53, 277 194 increased after cold, drought treatments, respectively, more than...
The MYC-like sequence CATGTG plays an important role in the dehydration-inducible expression of Arabidopsis thaliana EARLY RESPONSIVE TO DEHYDRATION STRESS 1 (ERD1) gene, which encodes a ClpA (ATP binding subunit caseinolytic ATP-dependent protease) homologous protein. Using yeast one-hybrid system, we isolated three cDNA clones encoding proteins that bind to 63-bp promoter region erd1, contains motif. These encode named ANAC019, ANAC055, and ANAC072, belong NAC transcription factor family....
Summary The OsNAC6 gene is a member of the NAC transcription factor family in rice. Expression induced by abiotic stresses, including cold, drought and high salinity. expression also wounding blast disease. A transactivation assay using yeast system demonstrated that functions as transcriptional activator, transient localization studies with OsNAC6–sGFP fusion protein revealed its nuclear localization. Transgenic rice plants over‐expressing constitutively exhibited growth retardation low...
ABSCISIC ACID-RESPONSIVE ELEMENT BINDING PROTEIN1 (AREB1) (i.e., ABF2) is a basic domain/leucine zipper transcription factor that binds to the abscisic acid (ABA)-responsive element (ABRE) motif in promoter region of ABA-inducible genes. Here, we show expression intact AREB1 gene on its own insufficient lead downstream genes under normal growth conditions. To overcome masked transactivation activity AREB1, created an activated form (AREB1DeltaQT). AREB1DeltaQT-overexpressing plants showed...
Summary Arabidopsis thaliana RD26 cDNA, isolated from dehydrated plants, encodes a NAC protein. Expression of the gene was induced not only by drought but also abscisic acid (ABA) and high salinity. The protein is localized in nucleus its C terminal has transcriptional activity. Transgenic plants overexpressing were highly sensitive to ABA, while ‐repressed insensitive. results microarray analysis showed that ABA‐ stress‐inducible genes are upregulated ‐overexpressed repressed plants....
Responses to water stress are thought be mediated by transcriptional regulation of gene expression via reversible protein phosphorylation events. Previously, we reported that bZIP (basic-domain leucine zipper)-type AREB/ABF (ABA-responsive element-binding protein/factor) transcription factors involved in ABA signaling under conditions Arabidopsis. The AREB1 is phosphorylated vitro ABA-activated SNF1-related kinase 2s (SnRK2s) such as SRK2D/SnRK2.2, SRK2E/SnRK2.6 and SRK2I/SnRK2.3...
Abstract The DEHYDRATION-RESPONSIVE ELEMENT BINDING PROTEIN2A (DREB2A) transcription factor controls water deficit–inducible gene expression and requires posttranslational modification for its activation. activation mechanism is not well understood; however, the stability of this protein in nucleus was recently found to be important Here, we report isolation Arabidopsis thaliana DREB2A-INTERACTING PROTEIN1 (DRIP1) DRIP2, C3HC4 RING domain–containing proteins that interact with DREB2A...
DREB1A/CBF3 and DREB2A are transcription factors that specifically interact with a cis-acting dehydration-responsive element (DRE), which is involved in cold- gene expression Arabidopsis (Arabidopsis thaliana). Overexpression of DREB1A improves stress tolerance to both freezing dehydration transgenic plants. In contrast, overexpression an active form results significant but only slight The downstream products for reported have similar putative functions, genes encoding enzymes carbohydrate...
Abstract The Arabidopsis (Arabidopsis thaliana) mutant stop1 (for sensitive to proton rhizotoxicity1) carries a missense mutation at an essential domain of the histidine-2-cysteine-2 zinc finger protein STOP1. Transcriptome analyses revealed that various genes were down-regulated in mutant, indicating STOP1 is involved signal transduction pathways regulating aluminum (Al)- and H+-responsive gene expression. Al hypersensitivity could be caused by down-regulation AtALMT1 ALUMINUM-ACTIVATED...
Summary Leaf senescence is the terminal phenotype of plant leaf development, and ethylene a major hormone inducing senescence. Recent studies have shown that abscisic acid (ABA) also induces However, detailed mechanisms ABA‐induced remain unclear. We focused on A subfamily stress‐responsive NAC (SNAC‐A) transcription factors, expression which induced by abiotic stresses, particularly ABA. Gene analysis revealed seven SNAC‐A genes including ANAC055 , ANAC019 ANAC072 / RD26 ANAC002 ATAF1...
Plants respond and adapt to drought stress in order survive under conditions. Several genes that at the transcriptional level have been described, but there are few reports on involved recovery from dehydration. Analysis of rehydration-inducible should help not only understand molecular mechanisms responses higher plants, also improve tolerance crops by gene manipulation. We used a full-length cDNA microarray containing ca. 7000 Arabidopsis cDNAs identified 152 genes. Venn diagram analysis...
We have developed a novel gain-of-function system that we named the FOX hunting (Full-length cDNA Over-eXpressing gene system). used normalized full-length and introduced each into Arabidopsis by in planta transformation. About 10 000 independent cDNAs were expressed independently under CaMV 35S promoter Arabidopsis. Each transgenic contained on average 2.6 clones was monitored various categories such as morphological changes, fertility leaf color. found 1487 possible mutants from 15 547...
The toxicity of proline (Pro) to plant growth has raised questions despite its protective functions in response environmental stresses. To evaluate Pro toxicity, we isolated an Arabidopsis T-DNA-tagged mutant, pdh, that had a defect dehydrogenase (AtProDH), which catalyzes the first step catabolism. pdh mutant showed hypersensitivity exogenous application ≤10 mM l-Pro, at wild-type plants grew normally. A dose-dependent increase internal free accumulation was observed during external supply....
Abscisic acid (ABA) signaling components play an important role in the drought stress response plants. Arabidopsis thaliana ENHANCED RESPONSE TO ABA1 ( ERA1 ) encodes β-subunit of farnesyltransferase and regulates ABA dehydration response. Therefore, is candidate gene for enhancing tolerance numerous crops. However, a rice Oryza sativa homolog has not been characterized previously. Here, we show that osera1 mutant lines, harboring CRISPR/Cas9-induced frameshift mutations, exhibit similar...
Polyamines (PAs) are ubiquitous, polycationic compounds that essential for the growth and survival of all organisms. Although PA-uptake system plays a key role in mammalian cancer plant survival, underlying molecular mechanisms not well understood. Here, we identified an Arabidopsis L-type amino acid transporter (LAT) family transporter, named RMV1 (resistant to methyl viologen 1), responsible uptake PA its analog paraquat (PQ). The natural variation PQ tolerance was determined 22 thaliana...
Transmission of plant viruses that replicate in the insect vector is known as persistent-propagative manner. However, it remains unclear whether such virus-vector relationships also occur between and other biological vectors arthropod mites. In this study, we investigated possible replication orchid fleck virus (OFV), a segmented rhabdovirus, within its mite (Brevipalpus californicus s.l.) using quantitative RT-qPCR, western blotting next-generation sequencing. Time-course RT-qPCR blot...
Yeast one-hybrid screening is widely used for the identification of transcription factors (TFs) that interact with specific DNA sequences. However, a whole cDNA library not efficient TFs because TF genes represent only small percentage clones in library. Here, we present development an yeast system using prey composed approximately 1,500 cDNAs Arabidopsis thaliana. This enabled us to isolate binds promoter sequence high efficiency, even when region gene interest was directly employed as...