A5037308395- Plant Molecular Biology Research
- Photosynthetic Processes and Mechanisms
- Plant Stress Responses and Tolerance
- Plant nutrient uptake and metabolism
- Light effects on plants
- Biocrusts and Microbial Ecology
- Plant Gene Expression Analysis
- CRISPR and Genetic Engineering
- Plant tissue culture and regeneration
- Plant Genetic and Mutation Studies
- Chromosomal and Genetic Variations
- Plant Reproductive Biology
- Algal biology and biofuel production
- Plant Micronutrient Interactions and Effects
- Photoreceptor and optogenetics research
- Legume Nitrogen Fixing Symbiosis
- Plant-Microbe Interactions and Immunity
- Genetics and Plant Breeding
- Metal-Catalyzed Oxygenation Mechanisms
- Polysaccharides and Plant Cell Walls
- Whipple's Disease and Interleukins
- Autoimmune and Inflammatory Disorders Research
- Genetic Mapping and Diversity in Plants and Animals
- Macrophage Migration Inhibitory Factor
- Retinopathy of Prematurity Studies
RIKEN Center for Sustainable Resource Science
2006-2022
Kanto Gakuin University
2010-2022
London Rebuilding Society
2016
Plant (United States)
2011-2014
Utrecht University
2011
Forschungszentrum Jülich
2011
Okinawa Institute of Science and Technology Graduate University
2011
Rothamsted Research
2011
National Center For Child Health and Development
2005-2010
RIKEN Center for Integrative Medical Sciences
2009
Genome integrity is continuously threatened by external stresses and endogenous hazards such as DNA replication errors reactive oxygen species. The damage checkpoint in metazoans ensures genome delaying cell-cycle progression to repair damaged or inducing apoptosis. ATM ATR (ataxia-telangiectasia-mutated -Rad3-related) are sensor kinases that relay the signal transducer Chk1 Chk2 downstream regulators. Plants also possess orthologs but lack obvious counterparts of Instead, plant-specific...
Because of ever-increasing environmental deterioration it is likely that the influx UV-B radiation (280-320 nm) will increase as a result depletion stratospheric ozone. Given this fact essential we better understand both rapid and adaptive responses plants to stress. Here, compare metabolic wild-type Arabidopsis with mutants impaired in flavonoid (transparent testa 4, tt4; transparent 5, tt5) or sinapoyl-malate (sinapoylglucose accumulator 1, sng1) biosynthesis, exposed short 24-h longer...
Nitrogen (N) is often a limiting nutrient whose availability determines plant growth and productivity. Because its low and/or not uniform over time space in nature, plants respond to variations N by altering uptake recycling mechanisms, but the molecular mechanisms underlying how these responses are regulated poorly understood. Here, we show that group of GARP G2-like transcription factors, Arabidopsis thaliana NITRATE-INDUCIBLE, GARP-TYPE TRANSCRIPTIONAL REPRESSOR1/HYPERSENSITIVE TO LOW...
We have developed a novel gain-of-function system that we named the FOX hunting (Full-length cDNA Over-eXpressing gene system). used normalized full-length and introduced each into Arabidopsis by in planta transformation. About 10 000 independent cDNAs were expressed independently under CaMV 35S promoter Arabidopsis. Each transgenic contained on average 2.6 clones was monitored various categories such as morphological changes, fertility leaf color. found 1487 possible mutants from 15 547...
Arabidopsis thaliana DEHYDRATION-RESPONSIVE ELEMENT BINDING PROTEIN2A (DREB2A) functions as a transcriptional activator that increases tolerance to osmotic and heat stresses; however, its expression also leads growth retardation reduced reproduction. To avoid these adverse effects, the of DREB2A is predicted be tightly regulated. We identified short promoter region represses under nonstress conditions. Yeast one-hybrid screening for interacting factors GROWTH-REGULATING FACTOR7 (GRF7). GRF7...
The strong regulation of plant carbon allocation and growth by trehalose metabolism is important for our understanding the mechanisms that determine yield, with obvious applications in crop improvement. To gain further insight on arrest feeding, we first established starch-deficient seedlings plastidic phosphoglucomutase1 mutant were similarly affected as wild type trehalose. Starch accumulation source cotyledons, therefore, did not cause starvation consequent growing zones. We then screened...
We have isolated two dominant mutants from screening approximately 50,000 RIKEN activation-tagging lines that short inflorescence internodes. The activation T-DNAs were inserted near a putative basic helix-loop-helix (bHLH) gene and expression of this was increased in the mutant lines. Overexpression bHLH produced original phenotype, indicating it responsible for mutants. Specific observed during seed development. loss-of-function mutation RETARDED GROWTH OF EMBRYO1 (RGE1) caused small...
Yeast one-hybrid screening is widely used for the identification of transcription factors (TFs) that interact with specific DNA sequences. However, a whole cDNA library not efficient TFs because TF genes represent only small percentage clones in library. Here, we present development an yeast system using prey composed approximately 1,500 cDNAs Arabidopsis thaliana. This enabled us to isolate binds promoter sequence high efficiency, even when region gene interest was directly employed as...
Summary Ectopic gene expression, or the gain‐of‐function approach, has advantage that once function of a is known can be transferred to many different plants by transformation. We previously reported method, called FOX hunting, involves ectopic expression Arabidopsis full‐length cDNAs in systematically generate mutants. This technology most beneficial for generating heterologous resource analysis useful plant functions. As an initial model we generated more than 23 000 independent transgenic...
Abstract Leaf trichomes in Arabidopsis thaliana develop through several distinct cellular processes, such as patterning, differentiation, and growth. Although recent studies have identified key transcription factors regulating early patterning differentiation steps, it is still largely unknown how these regulatory proteins mediate subsequent trichome development, which accompanied by rapid cell growth branching. Here, we report a novel mutation Arabidopsis, contrast with previously mutants,...
Potassium (K) is an essential macronutrient for plant growth and reproduction. HAK5, Arabidopsis high-affinity K transporter gene, plays important role in uptake. Its expression up-regulated response to deprivation rapidly down-regulated when sufficient levels have been re-established. To identify transcription factors regulating TF FOX (Transcription Factor Full-length cDNA Over-eXpressor) library containing approximately 800 was used transform lines previously transformed with a luciferase...
Profiling elemental contents in wheat grains and clarifying the underlying genetic systems are important for breeding of biofortified crops. Our objective was to evaluate potential 269 Afghan landraces increasing cultivars. The three major (Mg, K, P) minor (Mn, Fe, Zn) elements were measured by energy dispersive X-ray fluorescence spectrometry. Large variations observed among landraces. Marker-based heritability estimates low moderate, suggesting that complex quantitative traits. Genetic...
Abstract The fungal pathogen Rhizoctonia solani causes devastating diseases in hundreds of plant species. Among these, R. sheath blight, one the three major rice. To date, few genes have been reported that confer resistance to . Here, rice-FOX Arabidopsis lines identified as having a bacterial pathogen, Pseudomonas syringae pv tomato DC3000, and Colletotrichum higginsianum were screened for disease BROAD-SPECTRUM RESISTANCE2 ( BSR2 ), gene encoding an uncharacterized cytochrome P450 protein...
Summary Approximately 20 000 of the rice‐FOX Arabidopsis transgenic lines, which overexpress 13 rice full‐length cDNAs at random in , were screened for bacterial disease resistance by dip inoculation with Pseudomonas syringae pv. tomato DC3000 ( Pst DC3000). The identities overexpressed genes determined 72 lines that showed consistent after three independent screens. was verified 19 characterizing other same original hunting population or obtained reintroducing into ecotype Columbia floral...
Identification of gene function is important not only for basic research but also applied science, especially with regard to improvements in crop production. For rapid and efficient elucidation useful traits, we developed a system named FOX hunting (Full-length cDNA Over-eXpressor hunting) using full-length cDNAs (fl-cDNAs). A heterologous expression approach provides solution the high-throughput characterization functions agricultural plant species. Since fl-cDNAs contain all information...
Environmental stresses are major factors in limiting plant growth and crop production. To find genes improving salt tolerance, the screening of a large population transgenic Arabidopsis thaliana that expressed rice full-length cDNAs under salinity stress is reported here. In this study one isolated salt-tolerant lines, R07303 was analysed detail. An uncharacterized gene CHLOROPLAST PROTEIN-ENHANCING STRESS TOLERANCE (OsCEST) integrated R07303. Newly constructed overexpressed OsCEST or its...