A5003133683- Plant Molecular Biology Research
- Plant Reproductive Biology
- Plant nutrient uptake and metabolism
- Plant tissue culture and regeneration
- Photosynthetic Processes and Mechanisms
- Light effects on plants
- Plant Stress Responses and Tolerance
- Plant Gene Expression Analysis
- Microtubule and mitosis dynamics
- Polysaccharides and Plant Cell Walls
- Plant-Microbe Interactions and Immunity
- Pharmacy and Medical Practices
- Plant Physiology and Cultivation Studies
- Signaling Pathways in Disease
- Plant Pathogens and Fungal Diseases
- Ubiquitin and proteasome pathways
- Plant Diversity and Evolution
RIKEN Center for Sustainable Resource Science
2009-2025
The University of Tokyo
2025
RIKEN
2017
Kumamoto University
2015
Nagasaki Municipal Hospital
2015
Plant (United States)
2009
Abstract Highly efficient tissue repair is pivotal for surviving damage-associated stress. Plants generate callus upon injury to heal wound sites, yet regulatory mechanisms of remain elusive. Here, we identified WUSCHEL-RELATED HOMEOBOX 13 (WOX13) as a key regulator formation and organ adhesion in Arabidopsis (Arabidopsis thaliana). WOX13 belongs an ancient subclade the WOX family, previous study shows that orthologs moss Physcomitrium patens (PpWOX13L) are involved cellular reprogramming at...
Abstract Plant somatic cells reprogram and regenerate new tissues or organs when they are severely damaged. These physiological processes associated with dynamic transcriptional responses but how chromatin-based regulation contributes to wound-induced gene expression changes subsequent cellular reprogramming remains unknown. In this study we investigate the temporal dynamics of histone modifications H3K9/14ac, H3K27ac, H3K4me3, H3K27me3, H3K36me3, analyze their correlation at early time...
Abstract Plant cells exhibit remarkable plasticity of their differentiation states, enabling regeneration whole plants from differentiated somatic cells. How they revert cell fate and express pluripotency, however, remains unclear. In this study, we demonstrate that transcriptional activation auxin biosynthesis is crucial for reprogramming Arabidopsis (Arabidopsis thaliana) leaf Our data show interfering with the activity histone acetyltransferases dramatically reduces callus formation...
Plants can regenerate their bodies via de novo establishment of shoot apical meristems (SAMs) from pluripotent callus. Only a small fraction callus cells is eventually specified into SAMs but the molecular mechanisms underlying fate specification remain obscure. The expression WUSCHEL (WUS) an early hallmark SAM acquisition. Here, we show that WUS paralog, WUSCHEL-RELATED HOMEOBOX 13 (WOX13), negatively regulates formation in Arabidopsis thaliana . WOX13 promotes non-meristematic cell...
Abstract Leaf trichomes in Arabidopsis thaliana develop through several distinct cellular processes, such as patterning, differentiation, and growth. Although recent studies have identified key transcription factors regulating early patterning differentiation steps, it is still largely unknown how these regulatory proteins mediate subsequent trichome development, which accompanied by rapid cell growth branching. Here, we report a novel mutation Arabidopsis, contrast with previously mutants,...
Plants modify organ growth and tune morphogenesis in response to various endogenous environmental cues. At the cellular level, is often adjusted by alterations cell growth, but molecular mechanisms underlying this control remain poorly understood. In study, we identify DNA BINDING WITH ONE FINGER (DOF)-type transcription regulator OBF PROTEIN4 (OBP4) as a repressor of growth. Ectopic expression OBP4 Arabidopsis (Arabidopsis thaliana) inhibits resulting severe dwarfism repression genes...
ABSTRACT How plants determine the final size of growing cells is an important, yet unresolved, issue. Root hairs provide excellent model system with which to study this as their cell remarkably constant under environmental conditions. Previous studies have demonstrated that a basic helix-loop helix transcription factor ROOT HAIR DEFECTIVE 6-LIKE 4 (RSL4) promotes root hair growth, but how growth terminated not known. In study, we demonstrate trihelix GT-2-LIKE1 (GTL1) and its homolog DF1...
Upon detecting abiotic or biotic stress, plants generally reduce their growth, enabling resources to be conserved and diverted stress response mechanisms. In Arabidopsis thaliana, the AT-hook motif nuclear-localized (AHL) transcription factor family has been implicated in restricting rosette growth stress. However, mechanism by which AHLs repress rosettes is unknown. this study, we establish that SUPPRESSOR OF PHYTOCHROME B4-#3 (SOB3) other restrict petiole elongation antagonizing...
Root hair growth is tuned in response to the environment surrounding plants. While most previous studies focused on enhancement of root during nutrient starvation, few investigated presence excess nutrients. We report that post-embryonic wild-type Arabidopsis plants strongly suppressed with increasing availability, particularly case growth. further used gene expression profiling analyze how availability affects growth, and found RHD6 subfamily genes, which are positive regulators...
Many plants are able to regenerate upon cutting, and this process can be enhanced in vitro by incubating explants on hormone-supplemented media. While such protocols have been used for decades, little is known about the molecular details of how incubation conditions influence their efficiency. In study, we find that warm temperature promotes both callus formation shoot regeneration Arabidopsis thaliana. We show an increase regenerative capacity at higher temperatures correlates with...
Root hair (RH) cells can elongate to several hundred times their initial size, and are an ideal model system for investigating cell size control. Their development is influenced by both endogenous external signals, which combined form integrative response. Surprisingly, a low-temperature condition of 10°C causes increased RH growth in Arabidopsis monocots, even when the rest plant halted. Previously, we demonstrated strong correlation between response significant decrease nutrient...
Brassinosteroids (BRs) affect a wide range of developmental processes in plants and compromised production or signalling BRs causes severe growth defects. To identify new regulators plant organ growth, we searched the Arabidopsis FOX (Full-length cDNA Over-eXpressor gene) collection for mutants with altered size isolated two overexpression lines that display typical BR deficient dwarf phenotypes. The phenotype these lines, caused by an putative acyltransferase gene PIZZA (PIZ), was partly...
Plants form calluses and regenerate new organs when incubated on phytohormone-containing media. While accumulating evidence suggests that these regenerative processes are governed by transcriptional networks orchestrating wound response developmental transitions, it remains unknown if posttranslational regulatory mechanisms involved in this process. In study, we demonstrate SAP AND MIZ1 DOMAIN- CONTAINING LIGASE1 (SIZ1), an E3 ligase-catalyzing attachment of the SMALL UBIQUITIN-LIKE MODIFIER...
abstract Shoot regeneration involves reprogramming of somatic cells and de novo organization shoot apical meristems (SAMs). In the best-studied model system using Arabidopsis, is mediated by auxin-responsive pluripotent callus formation from pericycle or pericycle-like tissues according to lateral root development pathway. contrast, can be induced directly fully differentiated epidermal stem explants Torenia fournieri (Torenia), without intervening mass in culture with cytokinin; yet, its...
Abstract Injured plant somatic tissues regenerate themselves by establishing shoot or root meristems. In Arabidopsis (Arabidopsis thaliana), a two-step culture system ensures regeneration first promoting the acquisition of pluripotency and subsequently specifying fate new Although previous studies have reported importance phytohormones auxin cytokinin in determining meristems, whether how environmental factors influence this process remains elusive. study, we investigated impact light...
In patients with chronic kidney disease (CKD), cautious drug dose adjustment is required as adverse effects including drug-induced acute injury or intoxication may occur when an inappropriate of medicine administered. We herein performed family-pharmacy based trials for in which pharmacists evaluate renal function the using a hearing survey. our group it was revealed that 50.3% outpatients have impaired their average eGFR below 60 mL/min / 1.73 m2, and suggested we need to confirm degree...
Abstract Plant cells exhibit remarkable plasticity of their differentiation states, enabling regeneration whole plants from differentiated somatic cells. How they revert cell fate and express pluripotency, however, remains unclear. Here we show that transcriptional activation auxin biosynthesis is crucial for reprogramming Arabidopsis leaf We demonstrate interfering with the activity histone acetyltransferases dramatically reduces callus formation mesophyll protoplasts. Impaired acetylation...
Abstract SUPPRESSOR OF PHYTOCHROME B-4 #3 (SOB3) is a member of the AT-HOOK MOTIF CONTAINING NUCLEAR LOCALIZED (AHL) family transcription factors that are involved in light-mediated growth Arabidopsis thaliana , affecting processes such as hypocotyl elongation. The majority research on AHLs has been conducted continuous light. However, there unique molecular events promote short days (SD) compared to constant light conditions. Therefore, we investigated how affect elongation SD. Firstly,...
Summary Root hair growth is tuned in response to the environment surrounding plants. While most of previous studies focused on enhancement root during nutrient starvation, few investigated presence excess nutrients. We report that post-embryonic wild-type Arabidopsis plants strongly suppressed with increasing availability, particularly case growth. further used gene expression profiling analyze how availability affects growth, and found RHD6 subfamily genes, which are positive regulators...