A5027180485- Genomics and Chromatin Dynamics
- Invertebrate Immune Response Mechanisms
- CRISPR and Genetic Engineering
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Neurobiology and Insect Physiology Research
- RNA Research and Splicing
- Physiological and biochemical adaptations
- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- Mosquito-borne diseases and control
- Tissue Engineering and Regenerative Medicine
- Single-cell and spatial transcriptomics
- Galectins and Cancer Biology
- Animal Genetics and Reproduction
- Developmental Biology and Gene Regulation
- RNA Interference and Gene Delivery
- Gene Regulatory Network Analysis
- Insect symbiosis and bacterial influences
University of Manchester
2020-2024
Monash University
2016-2020
Australian Research Council
2016
Regulation of mRNA degradation is critical for a diverse array cellular processes and developmental cell fate decisions. Many methods determining half-lives rely on transcriptional inhibition or metabolic labelling. Here, we use non-invasive method estimating hundreds mRNAs in the early Drosophila embryo. This approach uses intronic exonic reads from total RNA-seq time series Gaussian process regression to model dynamics premature mature mRNAs. We show how regulation stability used establish...
Activation of the Drosophila receptor tyrosine kinase Torso (Tor) only at termini embryo is achieved by localized expression maternal gene Torso-like (Tsl). Tor has a second function in prothoracic gland as for prothoracicotropic hormone (PTTH) that initiates metamorphosis. Consistent with this tissue, Tsl also localizes to and influences developmental timing. Despite these commonalities, our studies we unexpectedly found tsl tor have opposing effects on body size; null mutants are smaller...
The regulation of mRNA decay is important for numerous cellular and developmental processes. Here, we use the patterning gene even-skipped (eve) in early Drosophila embryo to investigate contribution shaping mature expression patterns. Through P-body colocalisation analysis mathematical modelling live fixed imaging data, present evidence that eve stability regulated across stripe 2, with enhanced at edges stripe. To manipulate stability, perturbed by optogenetic degradation 5' 3'...
RNA is subject to many modifications, from small chemical changes such as methylation through conjugation of biomolecules glycans. As well these endogenously written also exposed damage induced by its environment. Certain clinical compounds are known drive covalent modifications with a growing appreciation for how affect function. To understand the regulation we need reliable, sensitive and rapid methodology their quantification. Thus, developed AquIRE applied it analysis drug-induced...
Dosage compensation, the balancing of X-linked gene expression between sexes and to autosomes, is critical an organism's fitness survival. In Drosophila, dosage compensation involves hypertranscription male X chromosome. Here, we use quantitative live imaging modeling at single-cell resolution study chromosome in Drosophila. We show that four genes studied undergo transcriptional bursting female embryos. Mechanistically, our data reveal upregulation primarily mediated by a higher RNA...
Abstract Pore-forming members of the membrane attack complex/perforin-like (MACPF) protein superfamily perform well-characterized roles as mammalian immune effectors. For example, complement component 9 and perforin function to directly form pores in Gram-negative pathogens or virally infected/transformed cells, respectively. In contrast, only known MACPF Drosophila melanogaster, Torso-like, plays crucial during development embryo patterning larval growth. Here, we report that addition these...
Blood cells, known as hemocytes in invertebrates, play important and conserved roles immunity, wound healing tissue remodelling. The control of hemocyte number is therefore critical to ensure these functions are not compromised, studies using Drosophila melanogaster proving useful for understanding how this occurs. Recently, the embryonic patterning gene, torso-like (tsl), was identified being required both normal development providing immunity against certain pathogens. Here, we report that...
Abstract Bone Morphogenetic Protein (BMP) signalling is tightly regulated extracellularly by specific protein-protein interactions. During dorsoventral patterning of vertebrate and invertebrate embryos, the conserved regulator Twisted gastrulation (Tsg) precisely modulates BMP binding Chordin/Short (Sog) to promote formation inhibitory Tsg-Sog/Chordin-BMP ternary complex. Here we elucidate mechanism which Tsg interacts with Sog/Chordin modulate extracellularly. Using AlphaFold predictions,...
Abstract Live imaging of transcription in the Drosophila embryo using MS2 or PP7 systems is transforming our understanding transcriptional regulation. However, insertion MS2/PP7 stem loops into endogenous genes requires laborious CRISPR genome editing. Here we exploit previously described Minos-mediated integration cassette (MiMIC) transposon system to establish a method for simply and rapidly inserting cassettes any thousands carrying MiMIC insertion. In addition generating variety loop...
Live imaging of transcription in the Drosophila embryo using MS2 or PP7 systems is transforming our understanding transcriptional regulation. However, insertion MS2/PP7 stem loops into endogenous genes requires laborious CRISPR genome editing. Here we exploit previously described Minos-mediated integration cassette (MiMIC) transposon system to establish a method for simply and rapidly inserting cassettes any thousands carrying MiMIC insertion. In addition generating variety loop donor fly...
Abstract Regulation of mRNA degradation is critical for a diverse array cellular processes and developmental cell fate decisions. Many methods determining half-lives rely on transcriptional inhibition or metabolic labelling. Here we use non-invasive method estimating hundreds mRNAs in the early Drosophila embryo. This approach uses intronic exonic reads from total RNA-seq time series Gaussian process regression to model dynamics premature mature mRNAs. We show how regulation stability used...
Summary Dosage compensation, the balancing of X linked gene expression between sexes and to autosomes, is critical an organism’s fitness survival. In Drosophila , dosage compensation involves hypertranscription male chromosome. Here we use quantitative live imaging modelling at single-cell resolution determine mechanism underlying chromosome in . We show that four genes studied undergo transcriptional bursting female embryos. Mechanistically our data reveal upregulation primarily mediated by...
Abstract Blood cells, known as hemocytes in invertebrates, play important and conserved roles immunity, wound healing tissue remodelling. The control of hemocyte number is therefore critical to ensure these functions are not compromised, studies using Drosophila proving useful for understanding how this occurs. Recently, the well characterised embryonic patterning gene, torso-like ( tsl ), was identified being required both normal providing immunity against certain pathogens. Here, we report...