A5033184399- RNA Research and Splicing
- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- Health and Conflict Studies
- Race, Genetics, and Society
- Forensic and Genetic Research
- Hybrid Renewable Energy Systems
- Genomics and Chromatin Dynamics
University of Manchester
2022-2025
Institute of Forensic Sciences
2012
Bexar County Medical Examiner's Office
2008
Regulation of mRNA degradation is critical for a diverse array cellular processes and developmental cell fate decisions. Many methods determining half-lives rely on transcriptional inhibition or metabolic labelling. Here, we use non-invasive method estimating hundreds mRNAs in the early Drosophila embryo. This approach uses intronic exonic reads from total RNA-seq time series Gaussian process regression to model dynamics premature mature mRNAs. We show how regulation stability used establish...
The regulation of mRNA decay is important for numerous cellular and developmental processes. Here, we use the patterning gene even-skipped (eve) in early Drosophila embryo to investigate contribution shaping mature expression patterns. Through P-body colocalisation analysis mathematical modelling live fixed imaging data, present evidence that eve stability regulated across stripe 2, with enhanced at edges stripe. To manipulate stability, perturbed by optogenetic degradation 5' 3'...
The ability to quantitatively study mRNA translation using SunTag imaging is transforming our understanding of the process. Here, we expand method new aspects regulation in Drosophila. Repression maternal hunchback (hb) posterior Drosophila embryo a textbook example translational control. Using quantitate SunTag-hb mRNAs, show that repression leaky as ~5% mRNAs are translated. In anterior embryo, and zygotic similar efficiency despite having different UTRs. We demonstrate can be used...
The ability to quantitatively study mRNA translation using SunTag imaging is transforming our understanding of the process. Here, we expand method new aspects regulation in Drosophila. Repression maternal hunchback (hb) posterior Drosophila embryo a textbook example translational control. Using quantitate SunTag-hb mRNAs, show that repression leaky as ∼5% mRNAs are translated. In anterior embryo, and zygotic similar efficiency despite having different UTRs. We demonstrate can be used...
Racial designations have evolved over the years to accommodate changes in our population demographics. The Federal Office of Management and Budget (OMB) has developed Standards assist with uniform documentation reporting race ethnicity. A minimum five defined categories currently exist for (American Indian or Alaska Native, Asian, Black African American, Native Hawaiian Other Pacific Islander White). Ethnicity is separated into either Hispanic origin Non-Hispanic origin. Current death...
Abstract Regulation of mRNA degradation is critical for a diverse array cellular processes and developmental cell fate decisions. Many methods determining half-lives rely on transcriptional inhibition or metabolic labelling. Here we use non-invasive method estimating hundreds mRNAs in the early Drosophila embryo. This approach uses intronic exonic reads from total RNA-seq time series Gaussian process regression to model dynamics premature mature mRNAs. We show how regulation stability used...