A5050598951- Biochemical and Structural Characterization
- Innovative Microfluidic and Catalytic Techniques Innovation
- Asymmetric Hydrogenation and Catalysis
- Mathematics, Computing, and Information Processing
- Handwritten Text Recognition Techniques
- Enzyme Catalysis and Immobilization
- Natural Language Processing Techniques
- Image Processing and 3D Reconstruction
- Algal biology and biofuel production
- Dental Implant Techniques and Outcomes
- Catalysis for Biomass Conversion
- Catalytic C–H Functionalization Methods
- Chemical Reactions and Isotopes
- Glycosylation and Glycoproteins Research
- Dental materials and restorations
- Botanical Research and Chemistry
- Analytical Chemistry and Chromatography
- Microfluidic and Capillary Electrophoresis Applications
- Nanofabrication and Lithography Techniques
- Molecular Junctions and Nanostructures
- Microbial Natural Products and Biosynthesis
- Biosensors and Analytical Detection
- Bone Tissue Engineering Materials
- Digital Humanities and Scholarship
- Polymer Surface Interaction Studies
RWTH Aachen University
2019-2024
DWI – Leibniz Institute for Interactive Materials
2019-2021
FH Aachen
2020
Thüringer Universitäts- und Landesbibliothek
2016
Microgels are an emerging class of "ideal" enzyme carriers because their chemical and process stability, biocompatibility, high loading capability. In this work, we synthesized a new type permanently positively charged poly(N-vinylcaprolactam) (PVCL) microgel with 1-vinyl-3-methylimidazolium (quaternization nitrogen by methylation N-vinylimidazole moieties) as comonomer (PVCL/VimQ) through precipitation polymerization. The PVCL/VimQ microgels were characterized respect to size, charge,...
This review focuses on the synthesis, concepts and applications of biocatalytic microgels (μ-Gel<italic>zymes</italic>) their impact in field sustainable catalysis.
Abstract A versatile peptide‐based toolbox for surface functionalization was established by a combination of universal material binding peptide (LCI‐anchor peptide) and sortase‐mediated bioconjugation (sortagging). This facilitates either as one‐ or two‐step strategy. In the case one‐step strategy, desired functionality directly introduced to LCI. For LCI modified with reactive group, which can be further functionalized (e.g., employing “click” chemistry). Sortagging LCI, sortase from...
Chemoenzymatic cascades enable reactions with the high productivity of chemocatalysts and selectivity enzymes. Nevertheless, combination these different fields catalysis is prone to mutual deactivation metal‐ biocatalysts. In this study, a one‐pot sequential two‐step catalytic cascade reaction was successfully implemented for synthesis methylene‐bridged bis(2‐substituted benzofuran). first step, palladium‐free Sonogashira used benzofuran derivative. subsequent formed 2‐substituted...
Sortase-mediated ligation (sortagging) is commonly performed using the Staphylococcus aureus sortase A (SaSrtA) that strictly recognizes N-terminal glycine residue. In this work, a rational design of Streptococcus pyogenes (SpSrtA) for improved transpeptidase activity toward different amino acid residues was conducted. The generated variant SpSrtA M3 (E189H/V206I/E215A) showed up to 6.6-fold (vs wild-type) enhanced catalytic efficiency. Additionally, retains specificity alanine, glycine,...
Staphylococcus aureus sortase A (SaSrtA) is widely used for site-specific protein modifications, but it lacks the robustness performing bioconjugation reactions at elevated temperatures or in presence of denaturing agents. Loop engineering and subsequent head-to-tail backbone cyclization SaSrtA yielded cyclized variant CyM6 that has a 7.5 °C increased melting temperature up to 4.6-fold resistance towards denaturants when compared parent rM4. gained 2.6-fold (vs. rM4) yield conjugate ligation...
The distribution and local catalytic activity of Cytochrome P450 enzymes immobilized in microgels by electrostatic interaction has been determined with a combination situ super-resolution fluorescence microscopy experiments.
Abstract Invited for the cover of this issue is Ulrich Schwaneberg and co‐workers at RWTH Aachen University DWI Leibniz‐Institut für Interaktive Materialien. The image depicts a loop engineered, backbone cyclized Staphylococcus aureus sortase A which shows enhanced robustness in site‐specific protein peptide modifications. Read full text article 10.1002/chem.202002740 .
Zusammenfassung: Im Rahmen des BMBF-geförderten Projekts KALLIMACHOS an der Universität Würzburg soll unter anderem die Textgrundlage für digitale Editionen per OCR gewonnen werden. Das Bearbeitungskorpus besteht aus deutschen, französischen und lateinischen Inkunabeln. Dieser Artikel zeigt, wie man mit bereits heute existierenden Methoden Programmen den Problemen bei von Inkunabeln entgegentreten kann. Hierzu wurde Universitätsbibliothek ein Verfahren erprobt, dem auf ausgewählten Werken...
The biological transformation is a growing part of current research in manufacturing and enables new possibilities the development innovative products. Modular functional assemblies based on building blocks potentially enable efficient economical functionalization component surfaces. Surface covers range industries applications with high economic technological potential societal relevance. In dentistry, inflammation gums near implant (periimplant infection) one most common complications...
The Cover Feature shows two gearwheels interlocking, symbolizing the key principle of cascade reaction. bottom gearwheel depicts Sonogashira cross-coupling initiated by a Cu scorpionate to synthesize 2-substituted benzofuran derivative. top illustrates P450 BM3 catalyzed formation N,N'-bis(benzofuran-2-ylmethyl)-N,N'-dimethylmethandiamine. This chemoenzymatic reaction sequence takes advantage transition metal catalysis engaged with enzymatic new valuable compounds. More information can be...
In order to apply Optical Character Recognition (OCR) historical printings of Latin script fully automatically, we report on our efforts construct a widely-applicable polyfont recognition model yielding text with Error Rate (CER) around 2% when applied out-of-the-box. Moreover, show how this can be further finetuned specific classes little manual and computational effort. The mixed or is trained wide variety materials, in terms age (from the 15th 19th century), typography (various types...