A5042122356- Enzyme Catalysis and Immobilization
- Microbial Metabolic Engineering and Bioproduction
- Pickering emulsions and particle stabilization
- Electrochemical sensors and biosensors
- Analytical Chemistry and Chromatography
- biodegradable polymer synthesis and properties
- Innovative Microfluidic and Catalytic Techniques Innovation
- Proteins in Food Systems
- Enzyme Production and Characterization
- Biochemical Acid Research Studies
- Enzyme Structure and Function
- Catalysis for Biomass Conversion
- Enzyme-mediated dye degradation
- Surfactants and Colloidal Systems
- Amino Acid Enzymes and Metabolism
- Biochemical and Molecular Research
- Biofuel production and bioconversion
- Microfluidic and Capillary Electrophoresis Applications
- Alcoholism and Thiamine Deficiency
- Metabolism and Genetic Disorders
- Metalloenzymes and iron-sulfur proteins
- Pancreatic function and diabetes
- Hydrogels: synthesis, properties, applications
- Electrohydrodynamics and Fluid Dynamics
- Dendrimers and Hyperbranched Polymers
TU Dresden
2016-2025
RWTH Aachen University
2002-2015
Technische Universität Berlin
2007-2014
University of York
2014
Institute of Molecular Biotechnology
2014
Max Planck Institute of Colloids and Interfaces
2011
Evonik (Germany)
2009-2011
Encapsulation of enzymes in Pickering emulsions results a large interfacial area the enzyme-containing aqueous phase for biocatalysis organic media. This immobilization technique minimizes enzyme inactivation through stabilizing immiscible liquids by particles, facilitates separation processes, and significantly increases catalytic performance both stable vulnerable enzymes. Thus, broad technical applicability can be envisioned.
The asymmetric reduction of ketones is performed by using lyophilized whole cells in neat substrates with defined water activity (aw). Ketones and alcohols prone to be unstable aqueous media can now converted via biocatalysis.
In cooperative catalysis, the combination of chemo- and biocatalysts to perform one-pot reactions is a powerful tool for improvement chemical synthesis. Herein, UiO-66-NH2 was employed stepwise immobilize Pd nanoparticles (NPs) Candida antarctica lipase B (CalB) fabrication biohybrid catalysts cascade reactions. Distinct from traditional materials, has robust but tunable structure that can be utilized with ligand exchange approach adjust its hydrophobicity, resulting in excellent catalyst...
Utilizing the biphasic system water/CO2 as a reaction and extraction medium is of rising importance, because its advantages green solvent combination. The problem predictable pH control in aqueous phase has to be addressed view optimized conditions. possible up approximately 6 by means buffer salts, resulting can predicted an algebraic equation.
ABSTRACT The synthesis of enantiopure α‐hydroxy ketones, particularly R ‐ and S ‐phenylacetylcarbinol (PAC), represents an important process in the pharmaceutical industry, serving as a pivotal step production drugs. Recently, two novel enzymes, ephedrine dehydrogenase (EDH) pseudoephedrine (PseDH), have been described. These enzymes enable specific reduction 1‐phenyl‐1,2‐propanedione (PPD) to ‐PAC ‐PAC, respectively. In this study, we transferred these into Pickering emulsions, which is...
Abstract The mechanical and leaching stability of enzymes adsorbed on macroporous carriers is an important issue for the technical applicability such biocatalysts. Both can considerably benefit from deposition silicone coating carrier surface. immobilized lipase Novozyme 435 (NZ435), as a model enzyme preparation, with different loadings was studied in detail by scanning electron microscopy (SEM) transmission (TEM), well energy‐dispersive X‐ray spectroscopy (EDX) BET isotherms, offers...
Abstract Background Laccases are blue multi-copper oxidases and catalyze the oxidation of phenolic non-phenolic compounds. There is considerable interest in using these enzymes for dye degradation as well synthesis aromatic produced at relatively low levels and, sometimes, isozymes native fungi. The investigation properties individual therefore becomes difficult. goal this study was to over-produce a previously reported laccase from Cyathus bulleri well-established expression system Pichia...
The aim of the present work is use a water soluble enzyme in an organic solvent, still with pronounced catalytic activity. Therefore, lipase B from Candida antarctica (CalB) immobilized within micron-sized thermosensitive p-NIPAM hydrogel particles using solvent exchange polar to solvents. absorbed amount CalB investigated at different immobilization temperatures. Confocal laser scanning microscopy (CLSM) shows that homogeneously distributed polymer network. An enhanced specific activity...
Although several strategies are now available to enzymatically cross-link linear polymers hydrogels for biomedical use, little progress has been reported on the use of dendritic same purpose. Herein, we demonstrate that horseradish peroxidase (HRP) successfully catalyzes oxidative cross-linking a hyperbranched polyglycerol (hPG) functionalized with phenol groups hydrogels. The tunable results in adjustable hydrogel properties. Because obtained materials cytocompatible, they have great...
The oxygen-tolerant NAD+-reducing soluble hydrogenase (SH) from Ralstonia eutropha H16 has been described as a promising catalyst for cofactor regeneration in biocatalysed reductions. In this study, the actual potential of enzyme application technical synthesis was evaluated. An overproduced, purified version coupled to carbonyl reductase Candida parapsilosis (CPCR), where it allowed an almost quantitative conversion model substrate; total turnover numbers (TTN: nproduct/nenzyme) up 143,666...
Abstract A new and versatile, crown ether appended, chiral supergelator has been designed synthesized based on the bis‐urea motif. The introduction of a stereogenic center improved its gelation ability significantly relative to achiral analogue. This low‐molecular‐weight gelator forms supramolecular gels in variety organic solvents. It is sensitive multiple chemical stimuli sol–gel phase transitions can be reversibly triggered by host–guest interactions. gel used trap enzymes release them...
Pickering emulsions (PEs) are particle-stabilized multiphase systems with promising features for synthetic applications. Described here is a novel, simplified set-up employing catalytically active whole cells simultaneous emulsion stabilization and reaction. In the stereoselective carboligation of benzaldehyde to (R)-benzoin catalyzed by lyase in E. coli, yielded maximum substrate conversion within very short time, while economizing material demand waste. Formation activity freshly produced...
One of the major limitations that prohibit more frequent use biocatalysis in production specialty and bulk chemicals is insufficient long-term stability commercially available enzyme preparations. Especially terms leaching carrier integrity under process conditions still an issue. Herein, we report on fabrication preparations superb mechanical outstanding towards leaching. For first time, such immobilisates have been obtained by deposition silicone coatings, from cheap building blocks simple...
We present a simple and versatile approach of using hydrogel microparticles to transfer both inorganic hydrophilic nanoparticles (NPs) such as CdTe quantum dots enzymes lipase B from Candida antarctica (CalB) organic media eventually encapsulate them in the gel by consecutive exchange water swollen with water-miscible solvents water-immiscible solvents. The entrapment is due their incompatibility soaked matrices surrounding environment, so obviates need for any chemical modification NP...