A5046145124- Peptidase Inhibition and Analysis
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Research on Leishmaniasis Studies
- Enzyme Production and Characterization
- Trypanosoma species research and implications
- Antibiotic Resistance in Bacteria
- Protein Hydrolysis and Bioactive Peptides
- Phytochemical compounds biological activities
- Bacterial Identification and Susceptibility Testing
- Glycosylation and Glycoproteins Research
- Natural Compound Pharmacology Studies
- Insect Resistance and Genetics
- Carbohydrate Chemistry and Synthesis
- RNA and protein synthesis mechanisms
- Influenza Virus Research Studies
- Biological Activity of Diterpenoids and Biflavonoids
- SARS-CoV-2 detection and testing
- Crystallization and Solubility Studies
- Molecular Biology Techniques and Applications
- Regulation of Appetite and Obesity
- Synthesis of Organic Compounds
- COVID-19 Clinical Research Studies
- Enzyme Structure and Function
- Antimicrobial Peptides and Activities
- COVID-19 diagnosis using AI
Bruker (Canada)
2025
Bruker (United States)
2022-2024
Universidade Federal de Minas Gerais
2023
Universidade Federal de São Paulo
2009-2016
Universidade Federal de Juiz de Fora
2012
Faculdade de Tecnologia e Ciências
2011
Fundação de Amparo à Pesquisa do Estado de São Paulo
2011
Universidade Federal de Campina Grande
2008
Universidade Federal de Alfenas
2008
We investigated the antimicrobial resistance profile and occurrence of Klebsiella pneumoniae carbapenemase (KPC)-producing Gram-negative rods in sewage samples obtained from a Brazilian teaching hospital wastewater treatment plant (WWTP) that receives it for treatment. identified multidrug-resistant bacteria as well KPC-2-producing Aeromonas spp. several Enterobacteriaceae species, including Kluyvera spp., effluent different sites WWTP. Most isolates showed blaKPC-2 gene harbored on...
X-linked hypophosphatemia (XLH/HYP)-with renal phosphate wasting, hypophosphatemia, osteomalacia, and tooth abscesses-is caused by mutations in the zinc-metallopeptidase PHEX gene (phosphate-regulating with homologies to endopeptidase on X chromosome). is highly expressed mineralized tissue cells. Inactivating lead distal effects (implying accumulation of a secreted, circulating phosphaturic factor) bone teeth mineralization-inhibiting, acidic serine- aspartate-rich motif (ASARM)-containing...
Recently, matrix-assisted laser desorption ionization–time-of-flight mass spectrometry (MALDI-TOF MS) was successfully applied for the detection of carbapenemase activity directly from Gram-negative colonies. Based on this principle, we evaluated performance MALDI-TOF MS rapid positive blood culture vials. A total 100 vials were randomly selected. assay results confirmed by carbapenemase-encoding genes. 110 bacterial isolates recovered. The identified 21 29 (72.4%) carbapenemase-producing...
ABSTRACT This study evaluates the accuracy of liquid chromatography-mass spectrometry (LC-MS) and matrix-assisted laser desorption ionization-time flight mass (MALDI-TOF) for detecting carbapenem hydrolytic activity among SPM-1-, GIM-1-, GES-5-producing Pseudomonas aeruginosa isolates OXA-143-, IMP-10-, OXA-58-producing Acinetobacter baumannii isolates. Class A B carbapenemase activities were rapidly detected by MALDI-TOF in a 2-h assay. However, an extended incubation time was necessary...
This work reports the purification of a novel alkaline protease enzyme from putative new thermophilic fungus Myceliophthora sp. The molecular weight was determined as 28.2 kDa by using MALDI-TOF MS and it inhibited PMSF indicating is serine-protease. optimum pH temperature were 9.0 40–45 °C, respectively. Mg only tested cation able to promote an increase activity. N-terminal sequence purified (GVVGVC) presented identity homology when compared other proteases fungi. study also provides...
N-glycosylation is implicated in cancers and aberrant recognized as a hallmark of cancer. Here, we mapped compared the site-specific N-glycoproteomes colon cancer HCT116 cells isogenic non-tumorigenic DNMT1/3b double knockout (DKO1) using Fbs1-GYR N-glycopeptide enrichment technology trapped ion mobility spectrometry. Many significant changes were revealed, providing molecular basis for further elucidation role protein function. display hypersialylation especially cell surface membrane...
Candida haemulonii is now considered a complex of two species and one variety: sensu stricto, duobushaemulonii the variety var. vulnera. Identification (ID) these relevant for epidemiological purposes therapeutic management, but different phenotypic commercial systems are unable to provide correct ID emergent pathogens. Hence, we evaluated MALDI-TOF MS performance C. species, analyzing isolates/strains pseudohaemulonii auris by platforms, their databases softwares. To differentiate sctricto...
SARS-CoV-2 infection poses a global health crisis. In parallel with the ongoing world effort to identify therapeutic solutions, there is critical need for improvement in prognosis of COVID-19. Here, we report plasma proteome fingerprinting that predict high (hospitalized) and low-risk (outpatients) cases COVID-19 identified by platform combines machine learning matrix-assisted laser desorption ionization mass spectrometry analysis. Sample preparation, MS, data analysis parameters were...
Composting is a way of transforming the organic waste into fertilizer, minimizing use inorganic compounds that may contaminate environment. This transformation result microorganism action, converting complex carbon sources energy. Enzymes are exported by microorganisms to surrounding environment mediate this process. The aiming present work prospect compost produced composting unit (OCU) Fundação Parque Zoológico de São Paulo (FPZSP) find novel starch hydrolyzing organisms (SHO) secrete...
Absorption and accumulation of bioavailable cyanobacterial metabolites (including cyanotoxins) are likely in fish after senescence the rupturing cells during bloom episodes. We determined toxicity cyanopeptides identified from two strains Microcystis (M. panniformis MIRS-04 M. aeruginosa NPDC-01) a freshwater tropical fish, Astyanax altiparanae (yellowtail tetra, lambari). Aqueous extracts both were prepared order to simulate realistic exposure these substances environment. Both selected...
We explored furin substrate requirements in addition to the motif R-X-K/R-R using synthetic fluorescent resonance energy transfer (FRET) decapeptides. These decapeptides were derived from cleavage sites viral coat glycoproteins and human bacterial protein precursors. The hydrolysis by of most was activated K+ ion, whereas kosmotropic anions Hofmeister series inhibitors. analysis hydrolytic activity showed that its efficiency is highly dependent on particular combinations amino acids at...
Cruzain is the major cysteine protease of Trypanosoma cruzi, infectious agent responsible for Chagas disease, and cruzain inhibitors display considerable antitrypanosomal activity. In present work we elucidated crystallographic data fukugetin, a biflavone isolated from Garcinia brasiliensis, investigated role this molecule as inhibitor. The kinetic analyses demonstrated that fukugetin inhibited papain by slow reversible type inhibition with KI 1.1 13.4 µM, respectively. However, was about 12...
The infections by protozoans of the genus Leishmania are a major worldwide health problem, with high endemicity in developing countries. drugs choice for treatment leishmaniasis pentavalent antimonials, which cause renal and cardiac toxicity. As part search new against leishmaniasis, we evaluated vitro protease inhibition activity extracts (hexanic, ethyl-acetate, ethanolic) fukugetin, bioflavonoid purified from ethyl-acetate extract pericarp fruit Garcinia brasiliensis, tree native to...
The malaria burden remains a major public health concern, especially in sub-Saharan Africa. complex biology of Plasmodium, the apicomplexan parasite responsible for this disease, challenges efforts to develop new strategies control disease. Proteolysis is fundamental process metabolism parasites, but roles proteases generating vasoactive peptides have not previously been explored. In present work, it was demonstrated by mass spectrometry analysis that Plasmodium parasites (Plasmodium...
We report the enzymatic properties and substrate specificity of human recombinant KLK3 in presence glycosaminoglycans (GAGs) sodium citrate. This salt is highly concentrated prostate its had a similar hydrolytic efficiency as chymotrypsin. In contrast to latter peptidase, activated by citrate efficiently hydrolyzed substrates containing R, H P at P1 position. Activated also cleaved peptides derived from bradykinin domain kininogen same sites kallikrein KLK1, but presented low kininogenase...
Kallikrein-related peptidases (KLKs) are trypsin-like and chymotrypsin-like serine proteases which expressed in several tissues. Their activity is tightly controlled by inhibitors including members of the protease Kazal-type (SPINK) family. These enzymes promising targets for treatment skin desquamation, inflammation cancer. Spink3 or caltrin I mouse pancreas males accessory glands resulting mature protein has been associated with different activities such as an inhibitor trypsin acrosin...
This work aimed includes performing the sclerotia chemical profile and evaluates their biological effects on mutagenesis, oxidative stress, cancer, malaria. A was determined by ultraperformance liquid chromatography mass spectrometry (UHPLC-HRMS) analysis dereplicating norditerpenoid dilactone, sclerolide, other compounds. The GI50 values to cancer cells (19.8 277.6 µg/mL) were higher than normal (16.05 µg/mL), meaning high cytotoxicity. Regarding results showed that all AcOET fraction...