A5060965609- Malaria Research and Control
- Mosquito-borne diseases and control
- Venomous Animal Envenomation and Studies
- Research on Leishmaniasis Studies
- Peptidase Inhibition and Analysis
- Liver Disease Diagnosis and Treatment
- Trypanosoma species research and implications
- Traditional and Medicinal Uses of Annonaceae
- Antimicrobial Peptides and Activities
- Invertebrate Immune Response Mechanisms
- Lipid Membrane Structure and Behavior
- Circadian rhythm and melatonin
- Synthesis and Catalytic Reactions
- Calpain Protease Function and Regulation
- Parasite Biology and Host Interactions
- Liver physiology and pathology
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Biochemical effects in animals
- Pneumocystis jirovecii pneumonia detection and treatment
- Drug Transport and Resistance Mechanisms
- Computational Drug Discovery Methods
- Sleep and Wakefulness Research
- Complement system in diseases
- Marine Sponges and Natural Products
- Lysosomal Storage Disorders Research
Universidade Federal de São Paulo
2015-2024
Instituto de Saúde
2022-2023
Universidade de São Paulo
2000-2007
Universidade Brasil
2007
Malaria parasites, Plasmodia, spend most of their asexual life cycle within red blood cells, where they proliferate and mature. The erythrocyte cytoplasm has very low [Ca2+] (<100 nM), which is different from the extracellular environment encountered by eukaryotic cells. absence Ca2+ usually incompatible with normal cell functions survival. In present work, we have tested possibility that Plasmodia overcome limitation posed intracellular through maintenance a high parasitophorous...
Carotenoids are widespread lipophilic pigments synthesized by all photosynthetic organisms and some nonphotosynthetic fungi bacteria. All carotenoids derived from the C40 isoprenoid precursor geranylgeranyl pyrophosphate, their chemical physical properties associated with light absorption, free radical scavenging, antioxidant activity. generally in well defined subcellular organelles, plastids, which also present phylum Apicomplexa, comprises a number of important human parasites, such as...
Abstract Mucopolysaccharidosis type I (MPS I) is caused by a deficiency of α‐iduronidase (IDUA), which leads to intralysosomal accumulation glysosaminoglycans. Patients with MPS present wide range clinical manifestations, but the mechanisms these alterations occur are still not fully understood. Genotype–phenotype correlations have been well established for I; hence, it likely that secondary and tertiary in cellular metabolism signaling may contribute physiopathology disease. The aim this...
Abstract: Calcium (Ca 2+ ) is a critical regulator of many aspects the Plasmodium reproductive cycle. In particular, intra‐erythrocyte parasites respond to circulating levels melatonin in process mediated partly by intracellular Ca . Melatonin promotes development and synchronicity parasites, thereby enhancing their spread worsening clinical implications. The signalling mechanisms underlying effects are not fully established, although both cyclic AMP (cAMP) have been implicated. Furthermore,...
Cellular defence against the formation of reactive oxygen species (ROS) involves a number mechanisms in which antioxidant enzymes such as catalase (CAT) and superoxide dismutase (SOD) play an important role. The relation between sleep deprivation oxidative stress has not yet been completely elucidated. Although some authors did find evidence this relationship, others found alterations markers response to deprivation. Thus, objective study was identify changes induced by activity gene...
Although we have at our disposal relatively low-cost drugs that can be prescribed for the treatment of malaria, prevalence resistant strains causative parasite has required development new drugs.
The malaria burden remains a major public health concern, especially in sub-Saharan Africa. complex biology of Plasmodium, the apicomplexan parasite responsible for this disease, challenges efforts to develop new strategies control disease. Proteolysis is fundamental process metabolism parasites, but roles proteases generating vasoactive peptides have not previously been explored. In present work, it was demonstrated by mass spectrometry analysis that Plasmodium parasites (Plasmodium...
Antimalarial drug resistance remains a major obstacle in malaria control. Evidence from Southeast Asia shows that to artemisinin combination therapy (ACT) is inevitable. Ethnopharmacological studies have confirmed the efficacy of curcumin against Plasmodium spp. Drug interaction assays between curcumin/piperine/chloroquine and curcumin/piperine/artemisinin combinations potential treatment interfere with ubiquitin proteasome system (UPS) were analyzed. In vivo was studied BALB/c mice infected...
Parasitic diseases like malaria are a major public health problem in many countries and disrupted sleep patterns an increasingly common part of modern life. The aim this study was to assess the effects paradoxical deprivation (PSD) rebound (RB) on malarial parasite infection mice. After PSD, one group immediately infected with parasites (PSD). two other PSD groups were allowed normally for either 24 h (24 RB) or 48 (48 RB). recovery periods, mice inoculated parasites. most affected by...
Despite the unquestionable importance of highly cationic feature several small polypeptides with high content positively charged amino acids for their biological activities, peptides do not necessarily have capacity to cross cell membranes. Interestingly, we found that crotamine, a amphiphilic peptide from South American rattlesnake venom, has unique cell-penetrating property affinity acidic vesicles, besides well-characterized antimicrobial and antitumoral activities. In spite remarkable in...
Plasmodium chabaudi malaria parasite organelles are major elements for ion homeostasis and cellular signaling also target antimalarial drugs. By using confocal imaging of intraerythrocytic parasites we demonstrated that the dye acridine orange (AO) is accumulated into P. subcellular compartments. The AO could be released from by collapsing pH gradient with K+/H+ ionophore nigericin (20 microM), or inhibiting H+-pump bafilomycin (4 microM). Similarly, in isolated loaded calcium indicator Fluo...
Background: Interaction of nuclear-distribution element-like 1 with disrupted-in-schizophrenia protein is crucial for neurite outgrowth/neuronal migration, and this interaction competitively inhibits peptidase activity. Nuclear-distribution activity reduced in antipsychotic-naïve first-episode psychosis medicated chronic schizophrenia, even lower treatment-resistant schizophrenia. Aims: The purpose study was to investigate a rat model overexpressing human non-mutant 1, consequent...
Malaria is a devastating disease caused by unicellular protozoan, Plasmodium, which affects 3.7 million people every year. Resistance of the parasite to classical treatments such as chloroquine requires development new drugs. To gain insight into mechanisms that control Plasmodium cell cycle, we have examined effects kinase inhibitors on blood-stage cycle rodent malaria parasite, chabaudi. In vitro incubation red blood cells for 17 h at 37ºC with led decrease in percent infected cells,...
Sleep is a physiological event that directly influences health by affecting the immune system, in which calcium (Ca(2+)) plays critical signaling role. We performed live cell measurements of cytosolic Ca(2+) mobilization to understand changes occur splenic cells after various periods sleep deprivation (SD).Adult male mice were subjected platform technique for different (from 12 72h) and intracellular fluctuations evaluated splenocytes confocal microscopy. also spleen evaluation flow...