A5079760249- Malaria Research and Control
- Mosquito-borne diseases and control
- Vector-borne infectious diseases
- Complement system in diseases
- Hemoglobinopathies and Related Disorders
- Pharmacogenetics and Drug Metabolism
- Primate Behavior and Ecology
- HIV Research and Treatment
- Parasite Biology and Host Interactions
- Bird parasitology and diseases
- Aquaculture disease management and microbiota
- Drug-Induced Hepatotoxicity and Protection
- Drug Transport and Resistance Mechanisms
- Genetic diversity and population structure
- Research on Leishmaniasis Studies
- Synthesis and Catalytic Reactions
- Forensic and Genetic Research
- Parasites and Host Interactions
- vaccines and immunoinformatics approaches
- Marine Sponges and Natural Products
- Pharmacological Effects and Toxicity Studies
- Blood groups and transfusion
- Invertebrate Immune Response Mechanisms
- Trypanosoma species research and implications
- Mollusks and Parasites Studies
Fundação Oswaldo Cruz
2015-2024
Faculdades Oswaldo Cruz
2021-2023
Emory University
2019-2022
University of Lisbon
2017
Instituto Evandro Chagas
2005
Universidade Federal de Mato Grosso
2005
Universidade Federal de Minas Gerais
2005
Malaria was eliminated from southern and southeastern Brazil over 50 years ago. However, an increasing number of autochthonous episodes attributed to Plasmodium vivax have recently been reported the Atlantic Forest region Rio de Janeiro state. As P vivax-like non-human primate malaria parasite species simium is locally enzootic, we performed a molecular epidemiological investigation determine whether zoonotic transmission occurring.We examined blood samples patients presenting with signs or...
Abstract Background In the last few years, study of microparticles (MPs) - submicron vesicles released from cells upon activation or apoptosis has gained growing interest in field inflammation and infectious diseases. Their role human malaria parasite Plasmodium vivax remains unexplored. Because acute been related to pro-inflammatory responses, main hypothesis investigated this was that infection is associated with elevated levels circulating MPs, which may play a during disease non-immune...
We examined the mitogenomes of a large global collection human malaria parasites to explore how and when Plasmodium falciparum P. vivax entered Americas. found evidence significant contribution African South Asian lineages present-day New World with additional appearing originate from Melanesia that were putatively carried by Australasian peoples who contributed genes Native Americans. Importantly, mitochondrial vivax-like species simium are shared platyrrhine monkeys humans in Atlantic...
The genetic background of the Brazilian population is mainly characterized by three parental populations: European, African, and Native American. aim this study was to overview ancestry estimates for different geographic regions analyze factors involved in these estimates. In systematic scoping review were included 51 studies, comprehending 81 populations 19 states from five Brazil. To reduce potential bias studies with sampling methods, we calculated mean weighted number individuals....
Although Plasmodium vivax relapses are classically associated with hypnozoite activation, it has been proposed that a proportion of these cases due to primaquine (PQ) treatment failure caused by polymorphisms in cytochrome P-450 2D6 (CYP2D6). Here, we present evidence CYP2D6 implicated PQ failure, which was reinforced findings genetically similar parasites, and may explain number relapses. Using computational approach, were predicted affect the activity through changes structural stability...
Activation of hypnozoites vivax malaria causes multiple clinical relapses, which contribute to the Plasmodium burden and continuing transmission. Artemisinin-based combination therapy (ACT) is effective against blood-stage P. but requires co-administration with primaquine achieve radical cure. The therapeutic efficacy depends on generation a therapeutically active metabolite via cytochrome P450 2D6 (CYP2D6). Impaired CYP2D6 metabolism has been associated treatment failure. This study...
In Brazil, two species of Plasmodium have been described infecting non-human primates, brasilianum and simium. These are morphologically, genetically immunologically indistinguishable from the human malariae vivax parasites, respectively. simium has observed naturally monkeys genera Alouatta Brachyteles in a restricted area Atlantic Forest south southeast regions Brazil. However, its reported geographical distribution diversity vertebrate hosts may be underestimated, since available data...
Plasmodium simium, a malaria parasite of non-human primates (NHP), was recently shown to cause zoonotic infections in humans Brazil. We sequenced the P. simium genome investigate its evolutionary history and identify any genetic adaptions that may underlie ability this switch between host species.
Summary The Duffy binding protein of Plasmodium vivax (DBP) is a critical adhesion ligand that participates in merozoite invasion human Duffy-positive erythrocytes. A small outbreak P. malaria, village located non-malarious area Brazil, offered us an opportunity to investigate the DBP immune responses among individuals who had their first and brief exposure malaria. Thirty-three participated five cross-sectional surveys, 15 with confirmed infection while residing (cases) 18 not experienced...
Although malaria cases have substantially decreased in Southeast Brazil, a significant increase the number of Plasmodium vivax-like autochthonous human has been reported remote areas Atlantic Forest past few decades Rio de Janeiro (RJ) state, including an outbreak during 2015-2016. The singular clinical and epidemiological aspects several cases, collectively with molecular genetic data, revealed that they were due to non-human primate (NHP) parasite simium; however, understanding malarial...
Plasmodium vivax infection is characterized by a dormant hepatic stage, the hypnozoite that activated at varying periods of time after clearance primary acute blood-stage, resulting in relapse. Differentiation between treatment failure and new infections requires characterization initial infections, relapses, clone multiplicity malaria infections.Parasite DNA obtained from primary/relapse paired blood samples 30 patients with P. Brazil was analyzed using 10 molecular markers (8...
Abstract Zoonotic malaria poses a unique problem for control. Autochthonous cases of human in the Atlantic Forest have recently been attributed to Plasmodium simium , parasite that commonly infects non-human primates this Brazilian biome. However, due its close similarity at both morphological and molecular level vivax diagnosis P. region remains problematic. Therefore, diagnostic assay able accurately identify is important surveillance. Based on mitochondrial genome sequences, primers were...
Abstract Malaria has provided a major selective pressure and modulated the genetic diversity of human genome. The variants Duffy Antigen/Receptor for Chemokines (DARC) gene have probably been selected by malaria parasites, particularly FY*O allele, which is fixed in sub-Saharan Africa confers resistance to Plasmodium vivax infection. Here, we showed influence genomic ancestry on distribution DARC genotypes highly admixed Brazilian population confirmed decreased susceptibility FY*A/FY*O...
The human malaria parasite Plasmodium vivax infects red blood cells through a key pathway that requires interaction between Duffy binding protein II (DBPII) and its receptor on reticulocytes, the antigen/receptor for chemokines (DARC). A high proportion of P. vivax-exposed individuals fail to develop antibodies inhibit DBPII-DARC interaction, genetic factors modulate this humoral immune response are poorly characterized. Here, we investigate if DBPII responsiveness could be HLA class...
The unexpected high proportion of submicroscopic malaria infections in areas with low transmission intensity challenges the control and elimination Americas. current PCR-based assays present limitations as most protocols still rely on amplification few-copies target gene. Here, hypothesis was that different plasmodial targets-ribosomal (18S rRNA) non-ribosomal multi-copy sequences (Pvr47 for Plasmodium vivax Pfr364 falciparum)-could increase chances detecting infection.A real-time PCR assay...
Plasmodium vivax relapse is one of the major causes sustained global malaria transmission. Primaquine (PQ) only commercial drug available to prevent relapses, and its efficacy dependent on metabolic activation by cytochrome P450 2D6 (CYP2D6). Impaired CYP2D6 function, caused allelic polymorphisms, leads therapeutic failure PQ as a radical cure for P. malaria. Here, we hypothesized that host immune response parasites modulates susceptibility recurrences in association with activity.
The dependence of Plasmodium vivax on invasion mediated by Duffy binding protein (DBP) makes this a prime candidate for development vaccine. However, the DBP-based vaccine might be hampered high variability ligand (DBPII), known to bias immune response toward specific DBP variant. Here, hypothesis being investigated is that analysis worldwide DBPII sequences will allow us determine minimum number haplotypes (MNH) included in broad coverage. For that, all available were compiled and MNH was...
Enhanced understanding of the transmission dynamics and population genetics for Plasmodium vivax is crucial in predicting emergence spread novel parasite phenotypes with major public health implications, such as new relapsing patterns, drug resistance increased virulence. Suitable molecular markers are required these genetic studies. Here, we focus on two groups that commonly used to analyse natural populations P. vivax. We use under selective pressure, instance, antigen-coding polymorphic...
Abstract Objective To investigate risk factors associated with the acquisition of antibodies against Plasmodium vivax Duffy binding protein (PvDBP) – a leading malaria vaccine candidate in well‐consolidated agricultural settlement Brazilian Amazon Region and to determine sequence diversity PvDBP ligand domain (DBP II ) within local parasite population. Methods Demographic, epidemiological clinical data were collected from 541 volunteers using structured questionnaire. Malaria parasites...
Blood infection by the simian parasite, Plasmodium simium, was identified in captive (n = 45, 4.4%) and wild Alouatta clamitans monkeys 20, 35%) from Atlantic Forest of southern Brazil. A single malaria symptomatic monkey presented clinical haematological alterations. high frequency vivax-specific antibodies detected among these monkeys, with 87% testing positive against P. vivax antigens. These findings highlight possibility as a zoonosis remaining its impact on epidemiology disease.
Leishmania infantum (syn. chagasi) is the etiological agent of visceral leishmaniasis (VL) in Brazil. The epidemiology VL poorly understood. Therefore, a more detailed molecular characterization at an intraspecific level certainly needed. Herein, three independent methods, multilocus microsatellite typing (MLMT), random amplification polymorphic DNA (RAPD) and simple sequence repeats-polymerase chain reaction (SSR-PCR), were used to evaluate genetic diversity 53 L. isolates from five...
Non-human primates (NHPs) as a source for Plasmodium infections in humans are challenge malaria elimination. In Brazil, two species of have been described infecting NHPs, brasilianum and simium. Both infective to man. resembles morphologically, genetically immunologically the human quartan malariae. naturally infects non-human from all New World monkey families large geographic area. family Callitrichidae only genus Saguinus has infected so far. The present study describes natural infection...
Plasmodium vivax Duffy binding protein (DBP) is functionally important in the erythrocyte invasion process and provides a logical target for vaccine-mediated immunity. In current study, we demonstrated that DBP naturally immunogenic different populations of Brazilian Amazon, proportions IgG positive subjects increased with exposure to malaria, reaching peak those long-term (> 15 years) Amazon area. This profile antibody response was significantly from one observed P. merozoite surface 1...
Abstract The function of the Plasmodium vivax Duffy binding protein (DBP) during erythrocyte invasion process is critical for successful parasite growth and pathogenesis in human infections. Although DBP subject intensive malaria vaccine research, investigations on functional proprieties anti‐DBP antibodies population have been limited [ Infect Immun 68 (2000) 3164]. In present study, we examined ability sera from different populations Brazilian Amazon – an area markedly unstable...