BackgroundMiltefosine has been used successfully to treat visceral leishmaniasis (VL) in India, but it was unsuccessful for VL a clinical trial Brazil.MethodsTo identify molecular markers that predict treatment failure whole genome sequencing of 26 L. infantum isolates, from cured and relapsed patients allowed GWAS analysis SNPs, gene chromosome copy number variations.FindingsA strong association identified (p = 0·0005) between the presence genetically stable Miltefosine Sensitivity Locus...

Background There is insufficient evidence to support visceral leishmaniasis (VL) treatment recommendations in Brazil and an urgent need improve current treatments. Drug combinations may be option. Methods A multicenter, randomized, open label, controlled trial was conducted five sites evaluate efficacy safety of (i) amphotericin B deoxycholate (AmphoB) (1 mg/kg/day for 14 days), (ii) liposomal (LAMB) (3 7 days) (iii) a combination LAMB (10 mg/kg single dose) plus meglumine antimoniate (MA)...

This study evaluated the performance of recombinant K39 (rK39) antigen in a immunochromatographic format (strip test) and crude enzyme-linked immunosorbent assay diagnosis Brazilian visceral leishmaniasis (VL) 128 consecutive patients with parasitologically proven infections (by microscopy and/or culture). For each patient, medical history was obtained complete physical examination performed. Controls included 10 healthy volunteers 50 other diseases: malaria (10), leprosy (9), Chagas’...

The results of five serologic tests (ELISA using promastigote antigen [ELISAp] and recombinant K39 [ELISArK39] K26 [ELISArK26] antigens, indirect immunofluorescence test [IIFT], immunochromatographic the rK39 [TRALd]) Montenegro skin (MST) were analyzed in 41 individuals treated for kala-azar living Porteirinha, Minas Gerais, Brazil. carried out 1 week to 12 years after specific treatment. All MSTs during 8 months treatment negative, whereas year, 28 (84.8%) positive. Negativity all was...

The search toward the establishment of novel serological tests for diagnosis leishmaniasis and proper differential may represent one alternative to invasive parasitological methods currently used identify infected individuals. In present work, we investigated potential use recombinant peroxidoxin (rPeroxidoxin) Leishmania (Viannia) braziliensis as a antigen immunodiagnosis human tegumentary (TL) visceral (VL) canine (CVL). Linear B-cell epitope mapping was performed polymorphic epitopes when...

In India, visceral leishmaniasis (VL) caused by Leishmania donovani has been successfully treated with miltefosine a cure rate of > 90%. To assess the efficacy and safety oral against Brazilian VL, which is infantum, phase II, open-label, dose-escalation study was conducted in children (aged 2-12 years) adolescent-adults 13-60 years). Definitive assessed at 6-month follow-up visit. The only 42% (6 14 patients) recommended treatment 28 days 68% (19 an extended 42 days. vitro susceptibility...

O objetivo deste estudo foi descrever o perfil epidemiológico dos acidentes ofídicos da macrorregião de saúde do Norte Estado Minas Gerais, Brasil. Foram analisadas informações sobre os relativos ao período compreendido entre janeiro 2002 a dezembro 2006, por meio bancos dados. Os resultados demonstraram 10.553 casos notificados, com ênfase para maior casuística em meses tempo quente e chuvoso, áreas urbanas (54,1%), faixa etária menor 20 anos (39,7%), acometendo mais homens estudantes...

Leishmania infantum (syn. chagasi) is the etiological agent of visceral leishmaniasis (VL) in Brazil. The epidemiology VL poorly understood. Therefore, a more detailed molecular characterization at an intraspecific level certainly needed. Herein, three independent methods, multilocus microsatellite typing (MLMT), random amplification polymorphic DNA (RAPD) and simple sequence repeats-polymerase chain reaction (SSR-PCR), were used to evaluate genetic diversity 53 L. isolates from five...

This study assessed the prevalence of visceral leishmaniasis in blood donors from three endemic regions Brazil and evaluated risk transmission by transfusion.Despite strong evidence through transfusion, real risk, an essential condition for taking effective measures to control this serious disease, has not been determined.A multicentre was performed highly areas. Candidates eligible their first donation underwent a socio-epidemiological interview, samples were collected enzyme-linked...

ABSTRACT The residual serological reactivity observed in patients cured of visceral leishmaniasis (VL) represents the major factor underlying low efficiency most anti- Leishmania approaches to assess posttherapeutic cure VL. Herein, we have described a detuned flow cytometry-based methodology detect anti-live (FC-ALPA-immunoglobulin G [IgG]) and anti-fixed (FC-AFPA-IgG) L. chagasi promastigote IgG, along titration curve (1:2,000 1:128,000), as tool late (12 months after treatment [12 mAT])...

BACKGROUND: Leishmaniasis is one of the most important infectious diseases worldwide. Our study can provide more knowledge about angiogenic and hypoxic events in leishmaniasis. We attempted to verify whether HIF-1 α protein expression may be associated VEGF-A, VEGFR2 MMP9 leishmanial lesions. OBJECTIVES: Besides understanding pathway, we performed correlation proteins. METHODS: In this study, gathered 54 paraffin blocks taken from skin lesions patients northern Minas Gerais, Brazil, with...

Visceral leishmaniasis (VL) is an ill-studied disease that endemic to several regions of Brazil. It often complicated by hemophagocytic lymphohistiocytosis (HLH), a potentially fatal disorder resulting from excessive non-malignant activation/proliferation T lymphocytes and macrophages. Considering the overlapping clinical laboratory characteristics these diseases, diagnosing HLH challenge. Therefore, tracking association between VL necessary in areas. Although can be inapparent resolve with...

O objetivo deste trabalho foi estudar a prevalência de adultos infectados por L. chagasi entre doadores sangue do Hemocentro Regional Montes Claros/MG .Realizou-se estudo epidemiológico, transversal e quantitativo, no período 16/09/08 13/11/08. Participaram da pesquisa 421 aptos na triagem clínica, sendo realizada imunofluorescência indireta para L.L.chagasi. Aqueles que apresentaram resultados positivos foram submetidos ao teste rápido antígeno-específico Leishmania donovani. A análise das...

To describe the clinical and epidemiological profile of pregnant women children treated at a reference outpatient clinic for congenital toxoplasmosis.Pregnant potentially exposed to Toxoplasma gondii were observed. Diagnoses made using serologic tests compatible with acute toxoplasmosis. Children presenting with: Toxoplasma-specific antibodies (IgM or IgA ascending IgG titers higher than maternal in first 3 months life) coupled toxoplasmosis symptoms; intracranial calcifications (by...

OBJETIVO: Conhecer as lesões oculares mais frequentes encontradas em crianças expostas à toxoplasmose congênita. MÉTODOS: Trata-se de um estudo retrospectivo, a partir uma coorte histórica, abordagem quantitativa. Foram avaliadas encaminhadas serviço infectologia pediátrica e inseridas apenas aquelas com diagnóstico confirmado A avaliação oftalmológica incluiu o mapeamento retina sequencial, sob dilatação pupilar. RESULTADOS: Das 58 presumivelmente ao risco doença durante gestação, 20...

We have recently developed a sensitive and specific urine-based antigen detection ELISA for the diagnosis of visceral leishmaniasis (VL). This assay used rabbit IgG chicken IgY polyclonal antibodies Leishmania infantum proteins iron superoxide dismutase 1 (Li-isd1), tryparedoxin1 (Li-txn1) nuclear transport factor 2 (Li-ntf2). However, limitations upscaling continuous supply. To circumvent these hurdles, we began to develop immortalized monoclonal antibodies. opted recombinant camelid VHHs...

Objetivo: Avaliar o perfil epidemiológico e vacinal dos casos suspeitos de sarampo estabelecendo a relação entre vacinação, sintomatologia internação hospitalar em municípios da macrorregião saúde Norte Minas Gerais. Metodologia: A Gerais possui 1.125.986 habitantes 54 municípios. Foi realizado levantamento distribuição do número todos os Gerais, no período 2016 2021, partir dados secundários, obtidos Sistema Informação Agravos Notificação. Foram analisados notificados 140 indivíduos com...

Introduction Parenteral antimony-based compounds are still the standard of care for cutaneous leishmaniasis (CL) treatment in many countries, despite their high toxicity. Previous studies showed that oral azithromycin could be an option CL treatment. The aim this study was to evaluate efficacy and safety (AZ) compared with injectable meglumine antimoniate (MA). Methods This a randomized, open-label, 2-arm, non-inferiority clinical trial. Treatment-naïve patients localized were treated MA...

Ascites formation is commonly associated with cancer, although it also present in other diseases. The aim of this study was to evaluate the usefulness vascular endothelial growth factor as a malignancy marker ascites different etiologies. levels VEGF 32 malignant and 31 non-malignant were determined by enzyme immunoassay (ELISA). significantly higher than (median=1265.9 pg/ml×114.6 pg/ml; p<0.0001). We observed 72% sensitivity 90% specificity, using 662 pg/ml cut-off value. Therefore,...