A5010379313- Research on Leishmaniasis Studies
- Trypanosoma species research and implications
- Parasites and Host Interactions
- Parasite Biology and Host Interactions
- Toxin Mechanisms and Immunotoxins
- Chronic Lymphocytic Leukemia Research
- Global Maternal and Child Health
- Eosinophilic Disorders and Syndromes
- Immune cells in cancer
- Acute Myeloid Leukemia Research
- T-cell and Retrovirus Studies
- Zoonotic diseases and public health
- Mycobacterium research and diagnosis
- Insect and Pesticide Research
- Endodontics and Root Canal Treatments
- Bacteriophages and microbial interactions
- Tumors and Oncological Cases
- Child Nutrition and Water Access
- Oral and Maxillofacial Pathology
- Infectious Diseases and Mycology
- Brucella: diagnosis, epidemiology, treatment
- Animal Disease Management and Epidemiology
- Synthesis and Biological Evaluation
- Cervical Cancer and HPV Research
- Leprosy Research and Treatment
Universidade Federal de Minas Gerais
2014-2024
PREtest Consult (United States)
2024
The Ohio State University
2020-2021
The Ohio State University Wexner Medical Center
2020-2021
Kenya Medical Research Institute
2020
Universidade Estadual de Montes Claros
2011-2013
Master's College
2012
Abstract Background Ibrutinib is a Bruton’s tyrosine kinase (BTK) and interleukin-2-inducible (ITK) inhibitor used for treating chronic lymphocytic leukaemia (CLL) other cancers. Although ibrutinib known to inhibit the growth of breast cancer cell in vitro, its impact on treatment metastasis unclear. Methods Using an orthotopic mouse model, we show that inhibits progression cancer. Results inhibited proliferation cells Ibrutinib-treated mice displayed significantly lower tumour burdens...
Background Trypanosoma cruzi strains are currently classified into six discrete typing units (DTUs) named TcI to VI. It is known that these DTUs have different geographical distribution, as well biological features. and TcII major found in patients from northern southern Latin America, respectively. Our hypothesis upon infection of human peripheral blood cells, Y strain (Tc II) Col cl1.7 I), cause distinct immunological changes, which might influence the clinical course Chagas disease....
Visceral leishmaniasis (VL) or kala-azar, the most severe form of leishmaniasis, can lead to death if not properly diagnosed and treated. Correct identification infected patients reservoirs is vital for controlling spread leishmaniasis. Current diagnostic kits show high sensitivity specificity, but also result in false negatives cross reactions with related parasitic infections. New methods greater accuracy are urgently needed diagnosis In this study, we aimed uncover a new highly effective...
Abstract MicroRNA-21 (miR-21) inhibits IL-12 expression and impairs the Th1 response necessary for control of Leishmania infection. Recent studies have shown that infection induces miR-21 in dendritic cells macrophages, inhibition restores expression. Because is known to be expressed due inflammatory stimuli a wide range hematopoietic cells, we investigated role regulating immune responses during visceral leishmaniasis (VL) caused by donovani We found was significantly elevated monocytes,...
Human infection with different species of Leishmania leads to distinct clinical manifestations, ranging from relatively mild cutaneous (Leishmania braziliensis) severe visceral infantum) forms leishmaniasis. Here, we asked whether in vitro human monocytes by strains responsible for manifestations early changes immunological characteristics and ability the host cells control Leishmania. We evaluated expression toll-like receptors MHC class II molecules, cytokines, following short-term L....
Visceral leishmaniasis (VL) is a serious and fatal disease caused by the parasites Leishmania infantum donovani . The gold standard diagnostic test for VL demonstration of or their DNA in spleen, lymph node, bone marrow aspirates.
BACKGROUND: The histopathology and immune responses of the healing process leishmaniasis are still poorly studied. OBJECTIVES: This study aimed to examine histopathological immunological aspects lesions patients with cutaneous before after different therapeutic methods. METHODS: We studied 23 individuals grouped according treatments: Glucantime, Glucantime + Leishvacin associated Bacillus Calmette-Guerin. For analysis changes present in dermis epidermis, histological sections were stained...
Abstract This study aimed to evaluate the performance of point-of-care circulating cathodic antigen (POC-CCA) test in a highly endemic area Brazil, comparing it Kato-Katz (KK) technique for sensitivity, specificity and intensity reaction relation parasitic load. The community Sergipe, participated study, providing three stool samples, one urine fingers tick blood sample was tested by enzyme-linked immunosorbent assay (ELISA). Sensitivity, specificity, positive predictive value, negative...
BACKGROUND: Leishmaniasis is one of the most important infectious diseases worldwide. Our study can provide more knowledge about angiogenic and hypoxic events in leishmaniasis. We attempted to verify whether HIF-1 α protein expression may be associated VEGF-A, VEGFR2 MMP9 leishmanial lesions. OBJECTIVES: Besides understanding pathway, we performed correlation proteins. METHODS: In this study, gathered 54 paraffin blocks taken from skin lesions patients northern Minas Gerais, Brazil, with...
The development of an accurate protein-based antigen detection assay for diagnosis active visceral leishmaniasis (VL) would represent a major clinical advance. VL is serious and fatal disease caused by the parasites Leishmania infantum donovani. gold standard confirmatory diagnostic test demonstration or their DNA from aspirates spleen, lymph node, bone marrow blood buffy coats. Here we describe production use monoclonal antibodies (mAbs) sensitive specific capture ELISA diagnosis. This...
Visceral leishmaniasis (VL) is caused by protozoa belonging to the Leishmania donovani complex and considered most serious fatal form among different types of leishmaniasis, if not early diagnosed treated. Among measures disease control stand out management infected dogs diagnosis appropriate treatment human cases. Several antigens have been characterized for use in VL diagnosis, them are recombinant kinesin-derived from L . infantum , as rK39 rKDDR. The main difference between these size...
We have recently developed a sensitive and specific urine-based antigen detection ELISA for the diagnosis of visceral leishmaniasis (VL). This assay used rabbit IgG chicken IgY polyclonal antibodies Leishmania infantum proteins iron superoxide dismutase 1 (Li-isd1), tryparedoxin1 (Li-txn1) nuclear transport factor 2 (Li-ntf2). However, limitations upscaling continuous supply. To circumvent these hurdles, we began to develop immortalized monoclonal antibodies. opted recombinant camelid VHHs...
Abstract Aims CD8 + T cells are important in mediating protective responses to intracellular pathogens. However, an uncontrolled response may lead pathology. The role of different clinical manifestations human leishmaniasis is controversial and poorly understood. We aim study the first exposure strains Leishmania , seeking correlate these findings with disease. Methods results have evaluated expression granzyme A, inflammatory anti‐inflammatory cytokines, as well CTLA‐4 by naïve exposed...
Abstract A 2‐year‐old captive gorilla ( Gorilla ) was diagnosed with visceral leishmaniasis in Brazil, and treated a single dose of liposomal amphotericin B, which resulted clinical cure. This is the first report gorillas, reported B treatment great apes.
Neutrophils are critical players in the first line of defense against pathogens and activation subsequent cellular responses. We aimed to determine effects interaction Trypanosoma cruzi with human neutrophils, using isolates two major discrete type units (DTUs) associated Chagas' disease Latin America (clone Col1.7G2 Y strain, DTU I II, respectively). Thus, we used CFSE-stained trypomastigotes measure neutrophil-T. interaction, neutrophil activation, cytokine expression death, after...
Abstract Background The purpose of this study was to evaluate the immunohistochemical profile cytokeratins (Ck) in pseudoepitheliomatous hyperplasia cutaneous leishmaniasis (CL). Methods tissue samples, with hyperplasia, were collected from 37 patients (age 6–85 years old) indolent ulcers skin confirmed as CL. lesions submitted immunolabeling for Ck pairs 4–13, 5–14, and 6–16. Results In CL cases, Ck4 Ck13 staining were, majority negative. Of them, 33 35 cases negative Ck13, respectively....
ABSTRACT Brucellosis is an important bacterial disease of global distribution with zoonotic potencial. Serological tests used in Brazil for diagnosis bovine brucellosis, including the Rose Bengal test (RBT), 2-mercaptoethanol (2-ME), fluorescent polarization (FPA), and complement fixation (FC), are based on smooth lipopolysaccharide antigen (S-LPS) Brucella abortus. The aim this study was to evaluate a recombinant BP26 protein as rapid lateral flow immunochromatographic assay (rBP26-LFIA)...
As leishmanioses são doenças negligenciadas de grande importância em saúde pública. Esta doença, causada por parasitos do gênero Leishmania e transmitidos insetos vetores pode se manifestar sob as formas visceral, cutânea muco-cutânea. A leishmaniose visceral ganhou importante destaque na abordagem da “Saúde Única” função interface humana, veterinária ambiental. Neste contexto, a detecção novas áreas com ocorrência casos autóctones LV canina é o marco para iniciar investigação, vigilância...