A5048341043- Research on Leishmaniasis Studies
- Immune Cell Function and Interaction
- Tuberculosis Research and Epidemiology
- Immune Response and Inflammation
- Cancer Immunotherapy and Biomarkers
- Trypanosoma species research and implications
- CAR-T cell therapy research
- Immunotherapy and Immune Responses
- Immune cells in cancer
- Autoimmune and Inflammatory Disorders Research
- Gut microbiota and health
- Epigenetics and DNA Methylation
- Advanced biosensing and bioanalysis techniques
- Parasites and Host Interactions
- Infectious Diseases and Tuberculosis
- Pediatric health and respiratory diseases
- Treatment of Major Depression
- Signaling Pathways in Disease
- Immune responses and vaccinations
- Inflammatory Myopathies and Dermatomyositis
- Cardiomyopathy and Myosin Studies
- Calcium signaling and nucleotide metabolism
- Metabolomics and Mass Spectrometry Studies
- Insect Pest Control Strategies
- Iron oxide chemistry and applications
Sharda University
2024-2025
The Ohio State University Wexner Medical Center
2020-2025
The Ohio State University
2020-2024
University of Delhi
2019-2024
All India Institute of Medical Sciences
2018-2020
Abstract Background Ibrutinib is a Bruton’s tyrosine kinase (BTK) and interleukin-2-inducible (ITK) inhibitor used for treating chronic lymphocytic leukaemia (CLL) other cancers. Although ibrutinib known to inhibit the growth of breast cancer cell in vitro, its impact on treatment metastasis unclear. Methods Using an orthotopic mouse model, we show that inhibits progression cancer. Results inhibited proliferation cells Ibrutinib-treated mice displayed significantly lower tumour burdens...
Leishmaniasis is a neglected protozoan disease affecting over 12 million people globally with no approved vaccines for human use. New World cutaneous leishmaniasis (CL) caused by L. mexicana characterized the development of chronic non-healing skin lesions. Using CRISPR/Cas9 technique, we have generated live attenuated centrin knockout (LmexCen-/-) parasites. Centrin cytoskeletal protein important cellular division in eukaryotes and, Leishmania, required only intracellular amastigote...
Although phagocytic cells are documented targets of Leishmania parasites, it is unclear whether other cell types can be infected. Here, we use unbiased single-cell RNA sequencing (scRNA-seq) to simultaneously analyze host and donovani transcriptomes identify annotate parasitized in spleen bone marrow chronically infected mice. Our dual-scRNA-seq methodology allows the detection heterogeneous populations. In spleen, monocytes macrophages dominant cells, while megakaryocytes, basophils,...
Abstract Background Forensic investigations often rely on DNA analysis from biological samples such as saliva, which provides a non-invasive and accessible source of DNA. However, efficient isolation high-quality saliva remains challenging due to the presence inhibitors mucins. In this study, method was evaluated for isolating using polyethyleneimine-functionalized iron-oxide nanoparticles, utilizing their high binding capacity magnetic properties. Saliva (200 µL) were obtained 20 healthy...
Therapeutic vaccines are a promising alternative for active immunotherapy different types of cancers. cancer aim to prevent immune system responses that not targeted at the tumors only, but also boost anti-tumor immunity and promote regression or eradication malignancy without, with minimal, adverse events. Clinical trial data have pushed development forward, US Food Drug Administration authorized first therapeutic vaccine. In present review, we discuss various approaches vaccines, along...
Gene therapy involves either the direct introduction of genetic material (DNA or RNA) into host cell (or organ), known as <em>in vivo</em> gene therapy, re-introduction modified target cells taken out host, <em>ex therapy. Cancer is mainly caused by non-functioning genes required for normal proliferation, and it has emerged leading cause death globally due to absence efficient safe therapies well early diagnostic modalities. Therapeutic trials using have shown that they...
Abstract MicroRNA-21 (miR-21) inhibits IL-12 expression and impairs the Th1 response necessary for control of Leishmania infection. Recent studies have shown that infection induces miR-21 in dendritic cells macrophages, inhibition restores expression. Because is known to be expressed due inflammatory stimuli a wide range hematopoietic cells, we investigated role regulating immune responses during visceral leishmaniasis (VL) caused by donovani We found was significantly elevated monocytes,...
Recent molecular subgrouping of ependymomas (EPN) by DNA methylation profiling has identified ST-EPN-RELA and PF-EPN-A subgroups to be associated with poor outcome. Snail/Slug are cardinal epithelial-to-mesenchymal transcription factors (EMT-TFs) overexpressed in several CNS tumors, including EPNs. A systematic analysis gene-sets/modules co-expressed Snail Slug genes using published expression microarray dataset (GSE27279)identified 634 for enriched TGF-β, PPAR PI3K signaling pathways, 757...
Human infection with the protozoan parasite Trypanosoma cruzi causes Chagas disease for which there are no prophylactic vaccines. Cyclophilin 19 is a secreted cis-trans peptidyl isomerase expressed in all life stages of cruzi. This protein insect stage leads to inactivation anti-parasitic peptides and transformation whereas intracellular amastigotes it participates generating ROS promoting growth parasites. We have generated mutant depleted expression Cyp19 by removal 2 3 genes encoding this...
Efficient extraction of DNA from biological fluids is crucial for applications in molecular biology, forensic science, and clinical diagnostics. However, traditional methods often require costly reagents lengthy procedures. This study aims to optimize the binding buffer composition using polyethyleneimine-coated iron oxide nanoparticles (PEI-IONPs), which offer dual benefits magnetic separation high DNA-binding efficiency.
We previously reported that M. tb on its own as well together with HIV inhibits macrophage apoptosis by upregulating the expression of Bcl2 and Inhibitor Apoptosis (IAP). In addition, recent reports from our lab showed stimulation either macrophages or BMDCs results in significant upregulation Bcl2. this report, we delineate role mediating defense responses dendritic cells (BMDCs) during mycobacterial infection. Inhibiting led to a decrease intracellular bacterial burden BMDCs. To further...
GNE gene-specific c.2179G>A(p.V727M) is a key alteration reported in patients with hereditary inclusion body myopathy (HIBM) and represents an ethnic founder mutation the Indian cohort. However, underlying role of this pathogenesis remains largely unknown. Thus, study, we aimed to access possible mechanisms V727M that could be leading myopathy. We evaluated various silico tools predict effect on pathogenicity, structural or interactions, induce Our results propose deleterious effects...
Staphylococcus aureus (S. aureus) is a gram-positive bacteria, which causes various fatal respiratory infections including pneumonia. The emergence of Methicillin-Resistance (MRSA) demands thorough understanding host-pathogen interactions. Here we report the role calcium in regulating defence responses S. macrophages. Regulating fluxes cells by different routes differentially governs expression T cell costimulatory molecule CD80 and Th1 promoting IL-12 receptor. Inhibiting influx from...
The GNE-associated V727M mutation is one of the most prevalent ethnic founder mutations in Asian HIBM cohort; however, its role inducing disease phenotype remains largely elusive. In this study, function hotspot was profoundly investigated. For this, mutation-specific altered expression profile and potential networks were explored. relevant muscular disorder-specific vivo studies patient data further analyzed, key molecular pathways identified. Our study found that GNEV727M resulted a...
Post translational modifications (PTMs) are exploited by various pathogens in order to escape host immune responses. SUMOylation is one of the PTMs which involved regulation a variety cellular However, effects on pathogenic bacteria largely remain elusive. We, therefore, investigated role regulating defense responses dendritic cells (DCs) during mycobacterial infection. Dendritic Cells female BALB/c mice and THP-1 macrophages were used. Western blotting was performed measure expression level...