A5059676224- Cancer, Hypoxia, and Metabolism
- Biochemical and Molecular Research
- Pancreatic and Hepatic Oncology Research
- Cancer Research and Treatments
- Cancer-related Molecular Pathways
- RNA modifications and cancer
- Cancer, Lipids, and Metabolism
- Diet and metabolism studies
- Nutrition and Health in Aging
- Metabolism, Diabetes, and Cancer
- Amino Acid Enzymes and Metabolism
- Immune Cell Function and Interaction
- Muscle Physiology and Disorders
- Fluorine in Organic Chemistry
- Genetics, Aging, and Longevity in Model Organisms
- Autophagy in Disease and Therapy
- Planarian Biology and Electrostimulation
- Epigenetics and DNA Methylation
- Glioma Diagnosis and Treatment
- Adipose Tissue and Metabolism
- Hepatitis B Virus Studies
- Pancreatic function and diabetes
- Nanoplatforms for cancer theranostics
- Diet, Metabolism, and Disease
- Mitochondrial Function and Pathology
University of Oklahoma Health Sciences Center
2022-2025
University of Nebraska Medical Center
2014-2024
OU Health Stephenson Cancer Center
2022-2023
Nebraska Medical Center
2023
D.Y. Patil University
2021
Institute of Medical Sciences
2021
K J Somaiya Medical College
2021
K. J. Somaiya Hospital & Research Centre
2019
International Centre for Genetic Engineering and Biotechnology
2012-2015
Susan Thompson Buffett Foundation
2015
Aberrant energy metabolism is a hallmark of cancer. To fulfill the increased requirements, tumor cells secrete cytokines/factors inducing muscle and fat degradation in cancer patients, condition known as cachexia. It accounts for nearly 20% all cancer-related deaths. However, mechanistic basis cachexia therapies targeting thus far remain elusive. A ketogenic diet, high-fat low-carbohydrate diet that elevates circulating levels ketone bodies (i.e., acetoacetate, β-hydroxybutyrate, acetone),...
Abstract Pancreatic adenocarcinoma is moderately responsive to gemcitabine-based chemotherapy, the most widely used single-agent therapy for pancreatic cancer. Although prognosis in cancer remains grim part due poor response therapy, previous attempts at identifying and targeting resistance mechanisms have not been very successful. By leveraging The Cancer Genome Atlas dataset, we identified lipid metabolism as metabolic pathway that significantly correlated with gemcitabine patients....
Abstract The ability of tumour cells to thrive in harsh microenvironments depends on the utilization nutrients available milieu. Here we show that pancreatic cancer-associated fibroblasts (CAFs) regulate cell metabolism through secretion acetate, which can be blocked by silencing ATP citrate lyase (ACLY) CAFs. We further acetyl-CoA synthetase short-chain family member 2 (ACSS2) channels exogenous acetate dynamic cancer epigenome and transcriptome, thereby facilitating survival an acidic...
// Shuping Yang 1,3,* , Lin Zhang 2,3,* Vinee Purohit 3 Surendra K. Shukla Xingcheng Chen Fang Yu 4 Kai Fu 5 Yuanhong Joyce Solheim Pankaj Singh Wei Song 1 and Jixin Dong Department of Oncology, Shandong Provincial Hospital affiliated with University, Jinan, Shandong, P. R. China 2 Radiation Qilu Eppley Institute for Research in Cancer, Fred Pamela Buffett Cancer Center, Omaha, NE, USA Biostatistics, School Public Health, Pathology Microbiology, University Nebraska Medical * These authors...
Purpose:MUC1, an oncogene overexpressed in multiple solid tumors, including pancreatic cancer, reduces overall survival and imparts resistance to radiation chemotherapies. We previously identified that MUC1 facilitates growth-promoting metabolic alterations cancer cells. The present study investigates the role of MUC1-mediated metabolism by utilizing cell lines vivo models.Experimental Design: used MUC1-knockdown -overexpressed line models for evaluating through vitro cytotoxicity,...
SIRT5 plays pleiotropic roles via post-translational modifications, serving as a tumor suppressor, or an oncogene, in different tumors. However, the role initiation and progression of pancreatic ductal adenocarcinoma (PDAC) remains unknown.
Dysregulation of polyamine metabolism has been implicated in cancer initiation and progression; however, the mechanism dysregulation is not fully understood. In this study, we investigated role MUC1, a mucin protein overexpressed pancreatic cancer, regulating metabolism. Utilizing patient data, noted positive correlation between MUC1 expression key pathway genes. Functional studies revealed that knockdown spermidine/spermine N1-acetyltransferase 1 ( SAT1 ), enzyme involved catabolism,...
// Surendra K. Shukla 1 , Aneesha Dasgupta 1, 2 Kamiya Mehla Venugopal Gunda Enza Vernucci Joshua Souchek Gennifer Goode Ryan King Anusha Mishra Ibha Rai Sangeetha Nagarajan Nina V. Chaika Fang Yu 3 Pankaj Singh 2, 4, 5 The Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, Omaha, 68198, USA Department Biochemistry Molecular Biology, Biostatistics, 4 Pathology Microbiology, Genetics Cell Biology Anatomy, Correspondence to: Singh, e-mail:...
// Surendra K. Shukla 1 , Venugopal Gunda Jaime Abrego Dhanya Haridas 2 Anusha Mishra Joshua Souchek Nina V. Chaika Fang Yu 3 Aaron R. Sasson 4 Audrey J. Lazenby 5 Surinder Batra Pankaj Singh 1, 2, 5, 6 The Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, Omaha, Nebraska, USA Department Biochemistry Molecular Biology, Biostatistics, Surgery, Pathology Microbiology, Genetics, Cell Biology Anatomy, Correspondence to: Singh, e-mail:...
Approximately one third of cancer patients die due to complexities related cachexia. However, the mechanisms cachexia and potential therapeutic interventions remain poorly studied. We observed a significant positive correlation between SIRT1 expression muscle fiber cross-sectional area in pancreatic patients. Rescuing Sirt1 by exogenous or pharmacological agents reverted cell–induced myotube wasting culture conditions mouse models. RNA-seq follow-up analyses showed cell–mediated loss induced...
Pancreatic cancer is the third leading cause of cancer-related deaths in USA. tumors are characterized by enhanced glycolytic metabolism promoted a hypoxic tumor microenvironment and resultant acidic milieu. The metabolic reprogramming allows cells to survive hostile microenvironments. Through analysis principal pathways, we identified specific metabolites that altered during pancreatic progression spontaneous (KPC) mouse model. Genetically engineered mice exhibited alterations PanINs...
Abstract Nutritional factors play crucial roles in immune responses. The tumor-caused nutritional deficiencies are known to affect antitumor immunity. Here, we demonstrate that pancreatic ductal adenocarcinoma (PDAC) cells can suppress NK-cell cytotoxicity by restricting the accessibility of vitamin B6 (VB6). PDAC actively consume VB6 support one-carbon metabolism, and thus tumor cell growth, causing deprivation microenvironment. In comparison, NK require for intracellular glycogen...
Therapeutic vaccines are a promising alternative for active immunotherapy different types of cancers. cancer aim to prevent immune system responses that not targeted at the tumors only, but also boost anti-tumor immunity and promote regression or eradication malignancy without, with minimal, adverse events. Clinical trial data have pushed development forward, US Food Drug Administration authorized first therapeutic vaccine. In present review, we discuss various approaches vaccines, along...
At present almost all the pectinolytic enzymes used for industrial applications are produced by fungi. There a few reports of pectinase production bacterial strains. Therefore, in study, seventy-four strains, isolated from soil and rotten vegetable samples, were screened polygalacturonase production. The strain PG-31, which gave maximum activity, was identified as Bacillus sphaericus (MTCC 7542). Maximal quantities when 16-hours-old inoculum at 7.5% (v/v) medium incubated shaking conditions...
The objective of this study was to design GE11 peptide (YHWYGYTPQNVI) linked micelles poly(ethylene glycol)-block-poly(2-methyl-2-carboxyl-propylene carbonate-graft-gemcitabine-graft-dodecanol (PEG-b-PCC-g-GEM-g-DC) for enhanced stability and target specificity gemcitabine (GEM) EGFR-positive pancreatic cancer cells. GE11-PEG-PCD/mPEG-b-PCC-g-GEM-g-DC mixed showed EGFR-dependent cellular uptake, cytotoxicity as compared scrambled HW12-PEG-PCD/mPEG-b-PCC-g-GEM-g-DC unmodified...
Pyrimidine nucleotide biosynthesis is a druggable metabolic dependency of cancer cells, and chemotherapy agents targeting pyrimidine metabolism are the backbone treatment for many cancers. Dihydroorotate dehydrogenase (DHODH) an essential enzyme in de novo pathway that can be targeted by clinically approved inhibitors. However, despite robust preclinical anticancer efficacy, DHODH inhibitors have shown limited single-agent activity phase 1 2 clinical trials. Therefore, novel combination...
Abstract Pancreatic ductal adenocarcinoma (PDAC) is frequently driven by activating mutations in KRAS, which promote tumor progression through sustained activation of the MAPK (RAS/RAF/MEK/ERK) signaling pathway. This study investigates metabolic vulnerabilities associated with PDAC examining reprogramming that occurs upon KRAS inhibition via targeting MEK/ERK/KRASG12D, and utilizing metabolomic, lipidomic, isotope-tracing approaches. Our findings reveal MEK/ERK/KRASG12D results enhanced...