A5041700915- Cancer, Hypoxia, and Metabolism
- Pancreatic and Hepatic Oncology Research
- Angiogenesis and VEGF in Cancer
- Cancer, Lipids, and Metabolism
- Cell Adhesion Molecules Research
- Cancer Research and Treatments
- Autophagy in Disease and Therapy
- Metabolism, Diabetes, and Cancer
- Glycosylation and Glycoproteins Research
- RNA modifications and cancer
- Glaucoma and retinal disorders
- Protease and Inhibitor Mechanisms
- Diet and metabolism studies
- Metabolomics and Mass Spectrometry Studies
- Glioma Diagnosis and Treatment
- Mitochondrial Function and Pathology
- Cancer-related Molecular Pathways
- Histone Deacetylase Inhibitors Research
- TGF-β signaling in diseases
- Endoplasmic Reticulum Stress and Disease
- Infective Endocarditis Diagnosis and Management
- Cancer-related molecular mechanisms research
- Streptococcal Infections and Treatments
- Epigenetics and DNA Methylation
- Flavonoids in Medical Research
University of Virginia
2023-2024
University of Nebraska Medical Center
2013-2022
Nebraska Medical Center
2016
Boys Town
2011-2013
Boys Town National Research Hospital
2011-2013
Creighton University
2011
Aberrant energy metabolism is a hallmark of cancer. To fulfill the increased requirements, tumor cells secrete cytokines/factors inducing muscle and fat degradation in cancer patients, condition known as cachexia. It accounts for nearly 20% all cancer-related deaths. However, mechanistic basis cachexia therapies targeting thus far remain elusive. A ketogenic diet, high-fat low-carbohydrate diet that elevates circulating levels ketone bodies (i.e., acetoacetate, β-hydroxybutyrate, acetone),...
Abstract Pancreatic adenocarcinoma is moderately responsive to gemcitabine-based chemotherapy, the most widely used single-agent therapy for pancreatic cancer. Although prognosis in cancer remains grim part due poor response therapy, previous attempts at identifying and targeting resistance mechanisms have not been very successful. By leveraging The Cancer Genome Atlas dataset, we identified lipid metabolism as metabolic pathway that significantly correlated with gemcitabine patients....
Purpose:MUC1, an oncogene overexpressed in multiple solid tumors, including pancreatic cancer, reduces overall survival and imparts resistance to radiation chemotherapies. We previously identified that MUC1 facilitates growth-promoting metabolic alterations cancer cells. The present study investigates the role of MUC1-mediated metabolism by utilizing cell lines vivo models.Experimental Design: used MUC1-knockdown -overexpressed line models for evaluating through vitro cytotoxicity,...
// Surendra K. Shukla 1 , Aneesha Dasgupta 1, 2 Kamiya Mehla Venugopal Gunda Enza Vernucci Joshua Souchek Gennifer Goode Ryan King Anusha Mishra Ibha Rai Sangeetha Nagarajan Nina V. Chaika Fang Yu 3 Pankaj Singh 2, 4, 5 The Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, Omaha, 68198, USA Department Biochemistry Molecular Biology, Biostatistics, 4 Pathology Microbiology, Genetics Cell Biology Anatomy, Correspondence to: Singh, e-mail:...
Highlights•Fascin is required for metabolic stress resistance in metastatic lung cancer•Fascin promotes mitochondrial OXPHOS through remodeling mtF-actin•Fascin augments the biogenesis of Complex I mtDNA homeostasis•The role fascin it to promote brain metastasisSummaryThe deregulation actin cytoskeleton has been extensively studied dissemination. However, post-dissemination dysregulation poorly understood. Here, we report that fascin, an actin-bundling protein, cancer colonization by...
// Surendra K. Shukla 1 , Venugopal Gunda Jaime Abrego Dhanya Haridas 2 Anusha Mishra Joshua Souchek Nina V. Chaika Fang Yu 3 Aaron R. Sasson 4 Audrey J. Lazenby 5 Surinder Batra Pankaj Singh 1, 2, 5, 6 The Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, Omaha, Nebraska, USA Department Biochemistry Molecular Biology, Biostatistics, Surgery, Pathology Microbiology, Genetics, Cell Biology Anatomy, Correspondence to: Singh, e-mail:...
Pancreatic cancer is the third leading cause of cancer-related deaths in USA. tumors are characterized by enhanced glycolytic metabolism promoted a hypoxic tumor microenvironment and resultant acidic milieu. The metabolic reprogramming allows cells to survive hostile microenvironments. Through analysis principal pathways, we identified specific metabolites that altered during pancreatic progression spontaneous (KPC) mouse model. Genetically engineered mice exhibited alterations PanINs...
ABSTRACT Oral streptococci, key players in oral biofilm formation, are implicated dysbiosis and various clinical conditions, including dental caries, gingivitis, periodontal disease, cancer. Specifically, Streptococcus anginosus is associated with esophageal, gastric, pharyngeal cancers, while mitis linked to However, no study has investigated the mechanistic links between these species cancer-related inflammatory responses. As an initial step, we probed innate immune response triggered by...
Mucin1 (MUC1), a glycoprotein associated with chemoresistance and an aggressive cancer phenotype, is aberrantly overexpressed in triple-negative breast (TNBC). Recent studies suggest that MUC1 plays role modulating cell metabolism thereby supports tumor growth. Herein, we examined the of metabolic reprogramming TNBC.MUC1 was stably MDA-MB-231 TNBC cells knocked down MDA-MB-468 cells. We performed liquid chromatography-coupled tandem mass spectrometry-assisted metabolomic analyses...
By standard convention, in order to increase the efficacy of metabolite detection from cell culture lysates, extracts a large quantity cells are utilized for multiple reaction monitoring-based metabolomic studies. Metabolomics small number offers potential economical alternative increased numbers, turn increasing utility culture-based metabolomics. However, effect reduced numbers on targeted profiling is relatively unstudied. Considering limited knowledge available feasibility and accuracy...
Pancreatic cancer cells overexpressing Mucin 1 (MUC1) rely on aerobic glycolysis and, correspondingly, are dependent glucose for survival. Our NMR metabolomics comparative analysis of control (S2–013.Neo) and MUC1-overexpressing (S2–013.MUC1) demonstrates that MUC1 reprograms glutamine metabolism upon limitation. The observed alteration in under limitation was accompanied by a relative decrease the proliferation compared with steady-state conditions. Moreover, induces G1 phase arrest where...
The inhibitory effects and mechanism(s) of type IV collagen α-6 chain-derived noncollagenous domain (α6[IV]NC1 or hexastatin) on elastin-derived peptide (EDP)-activated choroidal endothelial cell migration, kinase signaling, membrane 1 metalloproteinase (MT1-MMP) activation are explored.Mouse cells (MCECs) were incubated in media with soluble EDPs (kappa elastin, mouse Val-Gly-Val-Ala-Pro-Gly [VGVAPG] hexapeptide) for different time intervals without α6(IV)NC1. MCECs proliferation, tube...
Isocitrate dehydrogenase 1 (IDH1) is the most commonly mutated metabolic enzyme in human malignancy. A heterozygous genetic alteration, arginine 132, promotes conversion of α-ketoglutarate to D-2-hydroxyglutarate (2-HG). Although pharmacologic inhibitors mutant IDH1 are promising, resistance mechanisms targeted therapy not understood. Additionally, role wild-type (WT.IDH1) cancer requires further study. Recently, it was observed that regulatory RNA-binding protein, HuR (ELAVL1), protects...
Neuroblastoma are pediatric, extracranial malignancies showing alarming survival prognosis outcomes due to their resilience current aggressive treatment regimens, including chemotherapies with cisplatin (CDDP) provided in the first line of therapy regimens. Metabolic deregulation supports tumor cell drug-treated conditions. However, metabolic pathways underlying cisplatin-resistance least studied neuroblastoma. Our metabolomics analysis revealed that cisplatin-insensitive cells alter...
Pancreatic cancer (PC) is characterized by metabolic deregulations that often manifest as deviations in metabolite levels and aberrations their corresponding genes across the clinical specimens preclinical PC models. Cholesterol one of critical metabolites supporting PC, synthesized or acquired cells. Nevertheless, significance de novo cholesterol synthesis pathway has been controversial indicating need to reassess this PC. We utilized models patients cell lines mass spectrometry-based...
Age related macular degeneration and choroidal neovascularizationIn the eye, ocular tissues have an organized vascular supply confined to choroid, hyaloid inner layers of