A5072393194- Microbial Metabolic Engineering and Bioproduction
- Biofuel production and bioconversion
- Enzyme Production and Characterization
- Enzyme Catalysis and Immobilization
- Mass Spectrometry Techniques and Applications
- Pancreatic and Hepatic Oncology Research
- Potato Plant Research
- Metabolomics and Mass Spectrometry Studies
- Algal biology and biofuel production
- Bacterial Genetics and Biotechnology
- Biochemical and Molecular Research
- Multiple Myeloma Research and Treatments
- Cancer Research and Treatments
- Cancer, Hypoxia, and Metabolism
- Lung Cancer Research Studies
- Autophagy in Disease and Therapy
- Toxic Organic Pollutants Impact
- Microbial metabolism and enzyme function
- Sugarcane Cultivation and Processing
- Ubiquitin and proteasome pathways
- Viral Infectious Diseases and Gene Expression in Insects
- Effects and risks of endocrine disrupting chemicals
- Advances in Cucurbitaceae Research
- Protein Structure and Dynamics
- RNA and protein synthesis mechanisms
International Centre for Genetic Engineering and Biotechnology
2017-2024
University of Oklahoma Health Sciences Center
2023-2024
Centre for Advanced Bioenergy Research
2017-2021
Dysregulation of polyamine metabolism has been implicated in cancer initiation and progression; however, the mechanism dysregulation is not fully understood. In this study, we investigated role MUC1, a mucin protein overexpressed pancreatic cancer, regulating metabolism. Utilizing patient data, noted positive correlation between MUC1 expression key pathway genes. Functional studies revealed that knockdown spermidine/spermine N1-acetyltransferase 1 ( SAT1 ), enzyme involved catabolism,...
The impact of recombinant protein production (RPP) on host cells and the metabolic burden associated with it undermine efficiency system. This study utilized proteomics to investigate dynamics parent induced at different time points for RPP. results revealed significant changes in both transcriptional translational machinery that may have impacted burden, growth rate culture timing synthesis induction also played a critical role fate within cell, affecting product yield. identified...
Many studies have been carried out on the growth-modulating efficiency of plants by colonization an endophytic fungus Piriformospora indica. However, involving co-culture alga with fungal strains for enhanced biodiesel production are rare. In this study, interaction between P. indica and Parachlorella kessleri-I, a marine algal strain, was assessed at metabolic level. association fungus, biomass from 471.6 to 704 mg/L, fatty acid methyl ester (FAME) profile kessleri-I increased...
Previous epidemiological studies, largely conducted in high-income countries and cross-sectional, have suggested a relatively strong association between exposure to dichlorodiphenyldichloroethylene (DDE), metabolite of the pesticide dichlorodiphenyltrichloroethane (DDT), type 2 diabetes. DDT is widely used India prevalence diabetes there increasing, but these factors has not been explored date.The objective was estimate p,p' isomer DDE with incident India.A nested case-control study...
Cellulolytic enzymes capable of hydrolyzing plant biomass are secreted by microbial cells specifically in response to the carbon substrate present environment. These consist a catalytic domain, generally appended one or more non-catalytic Carbohydrate Binding Module (CBM), which enhances their activity towards recalcitrant biomass. In study, genome cellulolytic microbe Paenibacillus polymyxa A18 was annotated for presence CBMs and analyzed expression model polysaccharides Avicel, CMC xylan...
Abstract Background Sugarcane bagasse (SCB) is an abundant feedstock for second-generation bioethanol production. This complex biomass requires array of carbohydrate active enzymes (CAZymes), mostly from filamentous fungi, its deconstruction to monomeric sugars the production value-added fuels and chemicals. In this study, we evaluated repertoire proteins in secretome a catabolite repressor-deficient strain Penicillium funiculosum , Pf Mig1 88 response SCB induction examined their role...
Methotrexate (MTX) is a tight-binding dihydrofolate reductase (DHFR) inhibitor, used as both an antineoplastic and immunosuppressant therapeutic. MTX, like folate undergoes folylpolyglutamate synthetase-mediated γ-glutamylation, which affects cellular retention target specificity. Mechanisms of MTX resistance in cancers include decrease poly-γ-glutamylation upregulation DHFR. Here, we report series potent MTX-based proteolysis targeting chimeras (PROTACs) to investigate DHFR degradation...
Abstract Pancreatic Neuroendocrine tumors (PanNET) are challenging to diagnose and often detected at advanced stages due a lack of specific sensitive biomarkers. This study utilized proteomics as valuable approach for cancer biomarker discovery; therefore, mass spectrometry-based proteomic profiling was conducted on plasma samples from 12 subjects (3 controls; 5 Grade I, 4 II PanNET patients) identify potential proteins capable effectively distinguishing healthy controls. Data available via...
Abstract Carbon dioxide supplementation regulates the metabolism of photosynthetic organisms, initiating CO 2 fixation through central carbon and partitioning in various metabolic processes. It is important to understand molecular mechanisms control regulate biomass lipid production. In this context, we investigated physiological responses a freshwater microalga, Chlorella saccharophila , supplemented with very low (VLC) (300 ppm or 0.03%) high (HC) (30 000 ppm, 3% v/v), for its potential as...
Acyl-ACP reductase (AAR) is one of the two key cyanobacterial enzymes along with aldehyde deformylating oxygenase (ADO) involved in synthesis long-chain alkanes, a drop-in biofuel. The enzyme prone to aggregation when expressed Escherichia coli, leading varying alkane levels. present work attempts investigate crucial structural aspects AAR protein associated its stability and folding. Characterization by dynamic light scattering experiment intact mass spectrometry revealed that recombinantly...
Oleaginous yeast Rhodosporidium toruloides has great biotechnological potential and scientific interest, yet the molecular rationale of its cellular behavior to carbon nitrogen ratios with concurrent lipid agglomeration remains elusive. Here, metabolomics adaptations R. in response varying glucose concentrations have been investigated. In preliminary screening we found that 5% (w/v) was optimal for further analysis 3641. Hereafter, effect complementation increase evaluated different sources...
Abstract Background: The 5-year survival rate of pancreatic cancer is extremely low compared with other cancers, although it has increased to 12% in 2023 the United States. 80% ductal adenocarcinoma (PDAC) patients are not eligible for surgical procedure, and unfortunately, PDAC refractory standard-of-care chemotherapy. Thus, developing novel more effective treatment strategies urgently needed. We previously reported keratin 17 (K17) as a negative prognostic predictive biomarker, whose...
Abstract Background: Pancreatic ductal adenocarcinoma (PDAC) has emerged as the third leading cause of cancer-related deaths in U.S., part, due to intrinsic resistance main-line chemotherapeutic protocols. Our prior data showed that upregulation pyrimidine but not purine de novo biosynthesis is associated with shortened survival patients whose tumors expresses high keratin 17 (K17). Furthermore, we reported K17 expression confers chemoresistance by impacting inner mitochondrial membrane...
Abstract Background : Acyl-ACP reductase (AAR) is one of the two key cyanobacterial enzymes along with aldehyde deformylating oxygenase (ADO) involved in synthesis long-chain alkanes, a drop-in biofuel. The enzyme prone to aggregation when expressed E. coli , leading varying alkane levels. Intriguingly, structural characterization remains largely elusive as AAR alone failed form stable crystals, possibly due number intrinsically flexible random regions. present work attempts fill gap...