A5058911046- Biofuel production and bioconversion
- Microbial Metabolic Engineering and Bioproduction
- Enzyme Catalysis and Immobilization
- Malaria Research and Control
- Enzyme Production and Characterization
- Mosquito-borne diseases and control
- Fungal and yeast genetics research
- Complement system in diseases
- CRISPR and Genetic Engineering
- HIV Research and Treatment
- Algal biology and biofuel production
- Catalysis for Biomass Conversion
- Microbial bioremediation and biosurfactants
- Insect symbiosis and bacterial influences
- Computational Drug Discovery Methods
- Bacterial Genetics and Biotechnology
- Viral Infectious Diseases and Gene Expression in Insects
- Glycosylation and Glycoproteins Research
- Photosynthetic Processes and Mechanisms
- Insect and Arachnid Ecology and Behavior
- Studies on Chitinases and Chitosanases
- Genomics and Phylogenetic Studies
- Peptidase Inhibition and Analysis
- RNA and protein synthesis mechanisms
- Plant tissue culture and regeneration
International Centre for Genetic Engineering and Biotechnology
2016-2025
Centre for Advanced Bioenergy Research
2015-2024
International Centre for Genetic Engineering and Biotechnology
2004-2015
Razi University
2011
Rice University
2007-2008
Universidad del Valle
2005
Ministério da Saúde
2005
Fundação Oswaldo Cruz
2005
Universidad de Antioquia
2005
Jawaharlal Nehru University
1998-2002
Availability, low prices, and a high degree of reduction make glycerol an ideal feedstock to produce reduced chemicals fuels via anaerobic fermentation. Although metabolism in Escherichia coli had been thought be restricted respiratory conditions, we report here the utilization this carbon source absence electron acceptors. Cells grew fermentatively on exhibited exponential growth at maximum specific rate 0.040 +/- 0.003 h(-1). The fermentative nature was demonstrated through studies which...
The impact of recombinant protein production (RPP) on host cells and the metabolic burden associated with it undermine efficiency system. This study utilized proteomics to investigate dynamics parent induced at different time points for RPP. results revealed significant changes in both transcriptional translational machinery that may have impacted burden, growth rate culture timing synthesis induction also played a critical role fate within cell, affecting product yield. identified...
Reticulocyte invasion by
Summary Plasmodium vivax depends on interaction with the Duffy antigen/receptor for chemokines (DARC) invasion of human erythrocytes. The 140 kDa P. Duffy-binding protein (PvDBP) mediates DARC. receptor-binding domain PvDBP maps to its N-terminal, cysteine-rich region, region II (PvRII), which contains approximately 300 amino acid residues including 12 conserved cysteines. Using surface plasmon resonance, we show that binding PvRII DARC is a high-affinity constant (K(D)) 8.7 nM. minimal has...
ABSTRACT Insects living on wood and plants harbor a large variety of bacterial flora in their guts for degrading biomass. We isolated Paenibacillus strain, designated ICGEB2008, from the gut cotton bollworm basis its ability to secrete plant-hydrolyzing enzymes. In this study, we cloned, expressed, characterized two enzymes, β-1,4-endoglucanase (Endo5A) β-1,4-endoxylanase (Xyl11D), ICGEB2008 strain synthesized recombinant bifunctional enzymes based Endo5A Xyl11D. The gene encoding was...
ABSTRACT Identification and design of new cellulolytic enzymes with higher catalytic efficiency are a key factor in reducing the production cost lignocellulosic bioalcohol. We report here identification novel β-glucosidase (Gluc1C) from Paenibacillus sp. strain MTCC 5639 construction bifunctional chimeric proteins based on Gluc1C Endo5A, β-1,4-endoglucanase isolated earlier. The 448-amino-acid-long contained GH superfamily 1 domain hydrolyzed cellodextrin up to five-sugar chain length,...
Short-chain fatty acids (SCFAs), such as butyric acid, have a broad range of applications in chemical and fuel industries. Worldwide demand sustainable fuels chemicals has encouraged researchers for microbial synthesis SCFAs. In this study we compared three thioesterases, i.e., TesAT from Anaerococcus tetradius, TesBF Bryantella formatexigens TesBT Bacteroides thetaiotaomicron, production SCFAs Escherichia coli utilizing native acid (FASII) pathway modulated the genetic bioprocess parameters...
The quest for cheaper and better enzymes needed the efficient hydrolysis of lignocellulosic biomass has placed filamentous fungi in limelight bioprospecting research. In our search degraders, we identified a strain Penicillium funiculosum whose secretome demonstrates high saccharification capabilities. Our probe into fungus through qualitative label-free quantitative mass spectrometry based proteomics studies revealed abundance inducible CAZymes several nonhydrolytic accessory proteins....
Invasion of erythrocytes by malaria parasites is mediated specific molecular interactions. Plasmodium vivax completely dependent on interaction with the Duffy blood group antigen to invade human erythrocytes. The P. vivaxDuffy-binding protein, which binds during invasion, belongs a family erythrocyte-binding proteins that also includesPlasmodium falciparum sialic acid binding protein andPlasmodium knowlesi protein. receptor domains these lie in conserved, N-terminal, cysteine-rich region,...
Arthropods living on plants are able to digest plant biomass with the help of microbial flora in their guts. This study considered three arthropods from different niches - termites, pill-bugs and yellow stem-borers screened guts for cellulase producing microbes. Among 42 unique cellulase-producing strains, 50% belonged Bacillaceae, 26% Enterobacteriaceae, 17% Microbacteriaceae, 5% Paenibacillaceae 2% Promicromonosporaceae. The distribution families arthropod reflected differences food...
Background A phase I randomised, controlled, single blind, dose escalation trial was conducted to evaluate safety and immunogenicity of JAIVAC-1, a recombinant blood stage vaccine candidate against Plasmodium falciparum malaria, composed physical mixture two proteins, PfMSP-119, the 19 kD conserved, C-terminal region PfMSP-1 PfF2 receptor-binding F2 domain EBA175. Method Healthy malaria naïve Indian male subjects aged 18–45 years were recruited from volunteer database study site. Fifteen in...
There is an urgent requirement for second-generation bio-based industries economical yet efficient enzymatic cocktail to convert diverse cellulosic biomass into fermentable sugars. In our previous study, secretome of Penicillium funiculosum NCIM1228 showed high commercial potential by exhibiting hydrolyzing efficiency. To develop further as industrial workhorse, one the major genetic interventions needed global deregulation cellulolytic genes achieve higher enzyme production. Mig1 orthologs...
Sugar hydrolysates from lignocellulosic biomass are majorly composed of glucose and xylose that can be fermented to biofuels. Bacteria, despite having the natural ability consume unable it in presence due a carbon catabolite repression (CCR) mechanism. This leads overall reduced productivity as well incomplete utilization ethanol build-up utilization. In our effort develop strain for simultaneous fermentation into ethanol, we deleted ptsG ethanologenic E. coli SSK42 make deficient CCR...
Abstract Consolidated biosaccharification (CBS) of lignocellulosic biomass is evolving as a strategy to overcome recalcitrance and enhance bioconversion efficiency. However, low yield enzyme cocktail from lignocellulolytic microorganisms poses challenge the process. It therefore important identify explore microbial sources that can produce high titers enzymes, develop strategies for improved induction. This study investigated system Bacillus subtilis CFB‐09 using combination agro‐residues...
Plasmodium vivax and P. knowlesi use the Duffy antigen as a receptor to invade human erythrocytes. Duffy-binding ligands belong family of erythrocyte-binding proteins that bind erythrocyte receptors mediate invasion. Receptor-binding domains in lie conserved cysteine-rich regions called Duffy-binding-like domains. In present study, we report an analysis overall three-dimensional architecture based on mild proteolysis supportive-functional assays. Our experiments indicate these are built two...