Production of β-glucosidase from Fusarium oxysporum was investigated during degradation some cellulosic substrates (Avicel, α-cellulose, carboxymethyl cellulose (CMC), and methylcellulose). Optimized production using the substrate that supported highest yield enzyme examined over 192 h fermentation period varied pH 3.0-11.0. The produced characterized for its suitability industrial application. Methyl (177.5 U/mg) at 6.0 30°C 96 with liberation 2.121 μmol/mL glucose. crude had optimum...

There is an urgent requirement for second-generation bio-based industries economical yet efficient enzymatic cocktail to convert diverse cellulosic biomass into fermentable sugars. In our previous study, secretome of Penicillium funiculosum NCIM1228 showed high commercial potential by exhibiting hydrolyzing efficiency. To develop further as industrial workhorse, one the major genetic interventions needed global deregulation cellulolytic genes achieve higher enzyme production. Mig1 orthologs...

The enzymatic hydrolysis of cellulosic biomass by cellulases continues to be a significant bottleneck in the development second-generation biobased industries. While increasing efforts are being made obtain indigenous for hydrolysis, high production cost this enzyme remains crucial challenge affecting its wide availability efficient utilization materials. This is because it challenging an cocktail with balanced activity from single host. report describes annotation and structural analysis...

Abstract Background Sugarcane bagasse (SCB) is an abundant feedstock for second-generation bioethanol production. This complex biomass requires array of carbohydrate active enzymes (CAZymes), mostly from filamentous fungi, its deconstruction to monomeric sugars the production value-added fuels and chemicals. In this study, we evaluated repertoire proteins in secretome a catabolite repressor-deficient strain Penicillium funiculosum , Pf Mig1 88 response SCB induction examined their role...

Abstract Background Penicillium funiculosum NCIM1228 is a non-model filamentous fungus that produces high-quality secretome for lignocellulosic biomass saccharification. Despite having desirable traits to be an industrial workhorse, P. has been underestimated due lack of reliable genetic engineering tools. Tolerance towards common fungal antibiotics had one the major hindrances development transformation tools against fungi. In this study, we sought understand mechanism drug tolerance and...

Low-cost cellulase production is vital to sustainable second-generation biorefineries. The catabolically derepressed strain of Penicillium funiculosum NCIM1228 (PfMig1

The mechanism underlying the action of lignocellulolytic enzymes in biodegradation lignocellulosic biomass remains unclear; hence, it is crucial to investigate enzymatic interactions involved process. In this study, degradation corn cob by Sporothrix carnis and involvement were investigated over 240 h cultivation period. About 60% was achieved S. at end fermentation. yields hydrolytic enzymes, cellulase xylanase, higher than oxidative laccase peroxidase, 144 fermentation Maximum (854.4 U/mg)...

The production of second-generation fuels from lignocellulosic residues such as sugarcane bagasse (SCB) requires the synergistic interaction key cellulose-degrading enzymes and accessory proteins for their complete deconstruction to useful monomeric sugars. Here, we recombinantly expressed characterized unknown GH5 xylanase P. funiculosum (PfXyn5) in Pichia pastoris, which was earlier found our study be highly implicated SCB saccharification. PfXyn5 has a molecular mass ~ 55 kDa showed broad...

Bacillus amyloliquefaciens PFB-01 produced extracellular alkaline protease (385.25 U/ml) under shake flask conditions. Effects of process parameters such as cultivation period, pH, temperature, carbon and nitrogen sources on production were investigated for optimization. Production was optimized with 5.0% inoculum size in culture medium (pH 8.0) incubated 48 h at 37°C 200 rpm. A combination gelatin (0.75%) source glucose (0.5%) gave maximum yield (525.82U/mL) which 138% increase over enzyme...

Background: Agricultural activities generate large amounts of biomass residues and improper management the contributes to water, soil air pollution. The quest manage waste agricultural convert it into bioresource is therefore great concern. Methods: Fourteen fungal species isolated from decaying wood were identified production extracellular lignocellulolytic enzymes white soft rot fungi grown on corn cob, coconut husk, wheat bran, rice bran sawdust under submerged fermentation conditions was...

Abstract The response of filamentous fungi towards recalcitrant carbohydrates is majorly governed by transcriptional activators cellulase genes; however, little known about the downstream events beyond transcription. We show here in Penicillium funiculosum that increasing expression a activator CLR-2 catabolically derepressed strain, ΔMig1, didn’t exhibit synergistic effect on production unless Ca 2+ was simultaneously increased. RNA-seq screen for -activated kinases identified SNF1-AMPK and...

The enzymatic conversion of lignocellulosic biomass to bioethanol depends on efficient enzyme systems with β-glucosidase as one the key components. In this study, we performed in-depth profiling various β-glucosidases present in genome hypercellulolytic fungus Penicillium funiculosum using genomics, transcriptomics, proteomics, and molecular dynamics simulation approaches. Of eight genes identified P. genome, three were predicted be extracellular based signal peptide prediction abundance...

Abstract Lytic polysaccharide monooxygenases (LPMOs) are crucial industrial enzymes required in the biorefinery industry as well natural carbon cycle. These known to possess auxiliary activity produced by numerous bacterial and fungal species assist degradation of cellulosic biomass. In this study, we annotated performed structural analysis an uncharacterized thermostable LPMO from Penicillium funiculosum (PfLPMO9) attempt understand nature enzyme biomass degradation. PfLPMO9 exhibited 75%...

ABSTRACT Enzymatic lignocellulosic biomass conversion to bioethanol is dependent on efficient enzyme systems with β-glucosidase as a key component. In this study, we performed in-depth profiling of the various β-glucosidases present in genome hypercellulolytic fungus; Penicillium funiculosum using genomics, transcriptomics, proteomics and molecular dynamics simulation approaches. Of eight genes identified P . genome, three were found be extracellular, evidenced by presence signal peptides...

Abstract The production of second-generation fuels from lignocellulosic residues such as sugarcane bagasse (SCB) requires the synergistic interaction key cellulose degrading enzymes and accessory proteins for their complete deconstruction to useful monomeric sugars. Here, we recombinantly expressed characterized an unknown GH5 xylanase P. funiculosum ( Pf Xyn5) in Pichia pastoris , which was earlier found our study be highly implicated SCB saccharification. Xyn5 has a molecular mass ~ 55 kDa...