The present study investigates polyhydroxyalkanoate (PHA) production from lignin and its derivatives by a previously reported lignin-degrading bacterial strain Pandoraea sp. ISTKB. PHA was screened fluorescence microscopy flow cytometry using Nile red stain. biomass accumulation, while screening, found to be maximum on 4-hydroxybenzoic acid followed p-coumaric acid, vanillic 2,6-dimethoxyphenol, kraft after 96 h. Monomer composition analyzed gas chromatography-mass spectrometry (GC-MS)...

Lignin is a major component of plant biomass and recalcitrant to degradation due its complex heterogeneous aromatic structure. The biomass-based research mainly focuses on polysaccharides lignin discarded as waste with very limited usage. sustainability success polysaccharide-based biorefinery can be possible if utilized in improved ways minimal generation. Discovering new microbial strains understanding their enzyme system for are necessary conversion into fuel chemicals. Pandoraea sp....

Summary Flavonoids are important plant pigments and defense compounds; understanding the transcriptional regulation of flavonoid biosynthesis may enable engineering crops with improved nutrition stress tolerance. Here, we characterize R2R3‐MYB domain subgroup 7 transcription factor CaMYB39, which regulates flavonol primarily in chickpea trichomes. CaMYB39 overexpression was accompanied by a change flux availability for phenylpropanoid pathway, particularly biosynthesis. Lines overexpressing...

The present study was focused on evaluating the potential of Emblica officinalis against cariogenic properties Streptococcus mutans, a causative microorganism for caries. effect crude extract and ethanolic fraction from fruit analysed S. mutans. sub-MIC concentrations E. were evaluated its such as acid production, biofilm formation, cell-surface hydrophobicity, glucan sucrose-dependent independent adherence. Its architecture also investigated with help confocal scanning electron microscopy...

Objectives To evaluate the synergistic effect of Quercitrin and Deoxynojirimycin (DNJ) together with their individual inhibitory against virulence pathways Streptococcus mutans. Methodology MICs both compounds were determined by microdilution method, followed in vitrosynergy using checkerboard time kill assay. The nature interaction was classified as on basis fractional concentration index (FICI) value ≤0.5. Furthermore, activity DNJ evaluated individually combination various cariogenic...

Abstract Cool season crops face intermittent drought. Exposure to drought and other abiotic stresses is known increase tolerance of the plants against subsequent exposure such stresses. Storage environmental signals also proposed. Preexposure a dehydration shock improved adaptive response during treatment in cool crop chickpea (Cicer arietinum). We have identified 101 dehydration-inducible transcripts by repetitive rounds cDNA subtraction; differential DNA-array hybridization followed...

Localized cell death, known as the hypersensitive response (HR), is an important defense mechanism for neutralizing phytopathogens. The hallmark of HR oxidative burst produced by host plant. We aimed to identify genes necrotrophic chickpea blight fungus Ascochyta rabiei that are involved in counteracting stress. A subtractive cDNA library was constructed after menadione treatment, which resulted isolation 128 unigenes. reverse northern blot used compare transcript profiles H2O2, and sodium...

Abstract Constant evolutionary pressure acting on pathogens refines their molecular strategies to attain successful pathogenesis. Recent studies have shown that pathogenicity mechanisms of necrotrophic fungi are far more intricate than earlier evaluated. However, only a few explored fungal pathogens. Ascochyta rabiei is fungus causes devastating blight disease chickpea ( Cicer arietinum ). Here, we report 34.6 megabase draft genome assembly A. . The covered 99% the gene space and 4,259...

The WRKY genes have been identified as important transcriptional modulators predominantly during the environmental stresses, but they also play critical role at various stages of plant life cycle. We report identification domain (WD)-encoding from galegoid clade legumes chickpea (Cicer arietinum L.) and barrel medic (Medicago truncatula). In total, 78 98 WD-encoding were found in medic, respectively. Comparative analysis suggests presence both conserved unique WRKYs, expansion family M....

Native Bacillus sp. strain D5 coded as (Bar D5) has been isolated from the saffron corm that showed plant growth promotion (PGP) properties and also inhibits of rot causing Fusarium oxysporum R1 (Fox R1) in-vitro. Bar was more efficient PGP bacterium in comparison to earlier reported native bio-formulations by our group. Pot assays field evaluation confirmed its in-vivo efficacy for traits biocontrol activity well. trials were followed at traditional (Kishtwar) non-traditional (R.S Pura)...

Abstract The advancement of metabolomics has assisted in the identification various bewildering characteristics biological system. Metabolomics is a standard approach, facilitating crucial aspects system biology with absolute quantification metabolites using minimum samples, based on liquid/gas chromatography, mass spectrometry and nuclear magnetic resonance. metabolome profiling narrowed wide gaps missing information enhanced understanding spectrum plant–environment interactions by...

Ascochyta blight (AB) caused by the fungal pathogen rabiei is a serious foliar disease of chickpea (Cicer arietinum L.). Despite many genetic studies on chickpea-Ascochyta interaction, genome-wide scan for identification AB-associated quantitative trait loci (QTLs) and their gene(s) has not been accomplished. To elucidate narrow QTLs AB resistance, here, we report use multiple QTL-sequencing approach 2 sets extreme phenotype bulks derived from Cicer intraspecific interspecific crosses. Two...

Abstract Fungal pathogens deploy a barrage of secreted effectors to subvert host immunity, often by evading, disrupting, or altering key components transcription, defense signaling, and metabolic pathways. However, the underlying mechanisms their targets are largely unexplored in necrotrophic fungal pathogens. Here, we describe effector protein Ascochyta rabiei PEXEL-like Effector Candidate 25 (ArPEC25), which is necrotroph A. rabiei, causal agent blight disease chickpea (Cicer arietinum),...

The enzymatic hydrolysis of cellulosic biomass by cellulases continues to be a significant bottleneck in the development second-generation biobased industries. While increasing efforts are being made obtain indigenous for hydrolysis, high production cost this enzyme remains crucial challenge affecting its wide availability efficient utilization materials. This is because it challenging an cocktail with balanced activity from single host. report describes annotation and structural analysis...

Abstract The eukaryotic cytoskeleton is a complex scaffold consisting of actin filaments, intermediate and microtubules. Although fungi plants lack their dynamic structural network filaments microtubules regulates cell shape, division, polarity, vesicular trafficking. However, the specialized functions during plant–fungus interactions remain elusive. Recent reports demonstrate that plant responds to signal cues pathogen invasion through remodeling, thereby coordinating immune receptor...

Monitoring transcriptional reorganization triggered in response to a particular stress is an essential first step for the functional analysis of genes involved process. To characterize Cicer arietinum L. defence responses against Helicoverpa armigera feeding, transcript patterns elicited by both herbivore and mechanical wounding were profiled compared, application regulators was assessed. A combination approaches employed develop profiles, including suppression subtractive hybridization...