A5082082356- Research on Leishmaniasis Studies
- Trypanosoma species research and implications
- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Parasites and Host Interactions
- Atrial Fibrillation Management and Outcomes
- Virus-based gene therapy research
- CRISPR and Genetic Engineering
- Ferroptosis and cancer prognosis
- Probiotics and Fermented Foods
- Amoebic Infections and Treatments
- Innovation and Socioeconomic Development
- Animal Genetics and Reproduction
- Cancer Research and Treatments
- Adipose Tissue and Metabolism
- Antimicrobial Peptides and Activities
- Pediatric Hepatobiliary Diseases and Treatments
- Cardiac electrophysiology and arrhythmias
- Viral Infectious Diseases and Gene Expression in Insects
- Pancreatitis Pathology and Treatment
- Thyroid Cancer Diagnosis and Treatment
- Conducting polymers and applications
- Genetic Mapping and Diversity in Plants and Animals
- Protein Hydrolysis and Bioactive Peptides
- Protein Tyrosine Phosphatases
McGill University
2014-2025
Gansu Agricultural University
2018-2025
Zhejiang Chinese Medical University
2023-2025
Hangzhou First People's Hospital
2025
Chinese People's Liberation Army
2023-2024
Nanjing Agricultural University
2005-2024
Northwestern University
2019-2024
Zhongshan Hospital
2024
Renal Research Institute
2019-2020
Qingdao University
2015-2018
Recombinant adenoviruses containing the canine factor IX (FIX) cDNA were directly introduced in hind leg muscle of mice. We show that (i) nude mice, high expression (1-5 micrograms/ml plasma) FIX protein can be detected for > 300 days; (ii) contrast, was transient (7-10 days) normal mice; (iii) CD8+ lymphocytes could within 3 days infected tissue; (iv) use beta 2-microglobulin and immunoglobulin M heavy chain "knockout" mice showed lack sustained is due to cell-mediated humoral immune...
ABSTRACT The prokaryotic CRISPR (clustered regularly interspaced short palindromic repeat)-Cas9, an RNA-guided endonuclease, has been shown to mediate efficient genome editing in a wide variety of organisms. In the present study, CRISPR-Cas9 system adapted Leishmania donovani , protozoan parasite that causes fatal human visceral leishmaniasis. We introduced Cas9 nuclease into L. and generated guide RNA (gRNA) expression vectors by using rRNA promoter hepatitis delta virus (HDV) ribozyme. It...
Leishmaniasis is a neglected tropical disease caused by Leishmania protozoa transmitted infected sand flies. Vaccination through leishmanization with live major has been used successfully but no longer practiced because it resulted in occasional skin lesions. A second generation described here using CRISPR genome edited L. strain (LmCen-/-). Notably, LmCen-/- genetically engineered centrin gene knock-out mutant that antibiotic resistant marker free and does not have detectable off-target...
A central question in Leishmania research is why most species cause cutaneous infections but others fatal visceral disease. Interestingly, L. donovani causes both and leishmaniasis Sri Lanka. clinical isolates were therefore obtained from (CL-SL) (VL-SL) patients The CL-SL isolate was severely attenuated compared to the VL-SL for survival organs BALB/c mice. Genomic transcriptomic analysis argue that gene deletions or pseudogenes specific are not responsible difference disease tropism single...
Due to the strong stress resistance, causing food spoilage and toxin production of spore‐forming Bacillus species, their presence will be detrimental quality assurance dairy products. Impacts five strains ( licheniformis H1, B. H2, subtilis Z2, cereus Z6‐1 Z6‐2) originated from products on pasteurised yoghurt were investigated. Physiological characteristics strains, including growth, acid production, proteolysis, amylase extracellular polysaccharide biofilm formation, analysed. Additionally,...
Emerging evidence documents a key function for the forkhead transcription factor FoxO1 in cellular metabolism. Here, we investigate role of regulation fatty acid (FA) metabolism muscle cells. C2C12 cells expressing an inducible construct with either wild type or mutant form (FoxO1/TSS) refractory to protein kinase B inhibitory effects were generated. activation after myotube formation altered expression several genes FA Acyl-CoA oxidase and peroxisome proliferator-activated receptor δ mRNA...
Leishmania donovani is the etiologic agent of fatal visceral leishmaniasis in man. During their life cycle, exist as flagellated promastigotes within sandfly vector and nonflagellated amastigotes macrophage phagolysosomal compartment mammalian host. The transformation from to a critical step for establishment infection, molecular basis this poorly understood. To define amastigote survival host, we previously identified an stage-specific gene family termed “A2.” In present study, have...
Leishmania donovani is a protozoan parasite that exists as free-living promastigote in the sandfly insect vector and an amastigote inside mammalian host macrophage phagolysosome compartment. The L. A2 genes have been described previously developmentally expressed amastigotes but can be induced experimentally promastigotes by combination of pH temperature shifts, conditions mimic phagolysosomal compartment cell. Considering importance stage human infections, we examined molecular basis for...
Activation of Toll-like receptors (TLRs) on antigen-presenting cells the innate immune system initiates, amplifies, and directs antigen-specific acquired response. Ligands that stimulate TLRs therefore represent potential vaccine adjuvants. In present study, we determined whether imiquimod its related compound R848, which are TLR7 and/or TLR8 agonists, adjuvants when delivered topically, subcutaneously, or intramuscularly. Using Leishmania major infection model in BALB/c mice, vaccination...
CRISPR-Cas9-mediated genome editing has recently been adapted for Leishmania spp. parasites, the causative agents of human leishmaniasis. We have optimized this genome-editing tool by selecting cells with CRISPR-Cas9 activity through cotargeting miltefosine transporter gene; mutation gene leads to resistance. This strategy integrated into a triple guide RNA (gRNA) expression vector was used delete all 11 copies A2 multigene family; not previously possible traditional gene-targeting method....
Abstract Visceral Leishmaniasis (VL), a potentially fatal disease is caused by Leishmania donovani parasites with no vaccine available. Here we produced dermotropic live attenuated centrin gene deleted major ( LmCen −/− ) under Good Laboratory Practices and demonstrated that single intradermal injection confers robust durable protection against lethal VL transmitted naturally via bites of L. -infected sand flies prevents mortality. Surprisingly, immunogenicity characteristics revealed...
Leishmaniasis is a neglected protozoan disease affecting over 12 million people globally with no approved vaccines for human use. New World cutaneous leishmaniasis (CL) caused by L. mexicana characterized the development of chronic non-healing skin lesions. Using CRISPR/Cas9 technique, we have generated live attenuated centrin knockout (LmexCen-/-) parasites. Centrin cytoskeletal protein important cellular division in eukaryotes and, Leishmania, required only intracellular amastigote...
The A2 gene family is present in Leishmania donovani, which causes fatal visceral leishmaniasis human patients, but not major, cutaneous infections. genes L. donovani are stage specific and expressed at high levels the amastigote mammalian host, promastigote insect sandfly vector. tandem repeated with a distinct termed A2rel genes. In order to characterize structure function of A2-A2rel clusters, 5' 3' DNA sequences flanking cluster were isolated, sequenced used generate mutants through...
In Old World Leishmania infections, donovani is responsible for fatal visceral leishmaniasis, and L. major non-fatal cutaneous leishmaniasis in humans. The genetic differences between these species which govern the pathology or site of infection are not known. We have therefore carried out detailed analysis A2 loci because expressed but major, required survival organs by donovani. demonstrate that although contains gene regulatory sequences, multiple repeats exist protein coding regions...
Leishmania donovani is responsible for visceral leishmaniasis, a neglected and lethal parasitic disease with limited treatment options no vaccine. The study of L. has been hindered by the lack high-quality reference genome this can impact experimental outcomes including identification virulence genes, drug targets vaccine development. We therefore generated complete assembly deep sequencing using combination second generation (Illumina) third (PacBio) technologies. Compared to current...
The antibody response against an amastigote-specific protein (A2) from Leishmania donovani was investigated. Sera patients with trypanosomiasis and various forms of leishmaniasis were screened for anti-A2 antibodies. infected only L. or mexicana specifically recognized the A2 recombinant protein. These results consistent karyotype analyses which revealed that gene is conserved in strains. potential this antigen diagnosis further explored by screening a series sera obtained regions Sudan...
In this study, crucial genes and microRNAs (miRNAs) associated with the progression, staging, prognosis of papillary thyroid cancer (PTC) were identified.Four PTC datasets, including our own mRNA-sequencing (mRNA-seq) dataset three public datasets downloaded from Gene Expression Omnibus The Cancer Genome Atlas, used to analyze differentially expressed (DEGs) miRNAs (DEMs) between tumor tissues paired normal (control). ontology (GO) terms pathways these DEGs identified, protein-protein...
Due to differences in double-strand DNA break (DSB) repair mechanisms, CRISPR-Cas9 gene editing efficiency can vary greatly different organisms. In contrast mammalian cells, the protozoan parasite Leishmania uses microhomology-mediated end joining (MMEJ) and, occasionally, homology-directed (HDR) DSBs but lacks nonhomologous end-joining pathway. Here, we show that predominantly single-strand annealing (SSA) instead of MMEJ for DSB repairs (DSBR), resulting large deletions include multiple...
Dogs are the main reservoir host of Leishmania infantum, etiological agent zoonotic visceral leishmaniasis (ZVL). An effective vaccine against Canine Visceral Leishmaniasis (CVL) will help control and elimination ZVL. In this study, we evaluated in dogs safety, immunogenicity, efficacy a live attenuated major Centrin gene-deleted (LmCen−/−) as vaccine. Two doses (106 or 107) LmCen−/− were administered intradermally prime-boost regimen. Both induced equally high level IgG anti-Leishmania...