A5089250550- Trypanosoma species research and implications
- Research on Leishmaniasis Studies
- Parasites and Host Interactions
- Insect symbiosis and bacterial influences
- Biochemical and Molecular Research
- Helminth infection and control
- Parasite Biology and Host Interactions
- vaccines and immunoinformatics approaches
- Cancer Genomics and Diagnostics
- Malaria Research and Control
- Child Nutrition and Water Access
- Global Maternal and Child Health
- Antimicrobial Peptides and Activities
- Insect Resistance and Genetics
- Biotechnology and Related Fields
- Pneumocystis jirovecii pneumonia detection and treatment
- Acute Myeloid Leukemia Research
- Chromosomal and Genetic Variations
Universidade Federal de Minas Gerais
2018-2024
Sabin Medicina Diagnostica (Brazil)
2024
Aneuploidy is widely observed in both unicellular and multicellular eukaryotes, usually associated with adaptation to stress conditions. Chromosomal duplication stability a tradeoff between the fitness cost of having unbalanced gene copies potential gained from increased dosage specific advantageous genes. Trypanosomatids, family protozoans that include species cause neglected tropical diseases, are relevant group study aneuploidies. Their life cycle has several stressors could select for...
Trypanosoma cruzi, the etiologic agent of Chagas disease, is currently divided into six discrete typing units (DTUs), named TcI-TcVI. TcII among major DTUs enrolled in human infections South America southern cone, where it associated with severe cardiac and digestive symptoms. Despite importance disease epidemiology pathology, so far, no genome-wide comparisons mitochondrial nuclear genomes field isolates have been performed to track variability evolution this DTU endemic regions.In present...
Trypanosoma cruzi is the causative agent of Chagas disease, a neglected tropical disease that affects approximately 6 to 8 million people and for which there no effective treatment or vaccine. The parasite expresses family surface proteins, named trans-sialidases, responsible transferring sialic acid from host glycoconjugates mucins.
Although aneuploidy usually results in severe abnormalities multicellular eukaryotes, recent data suggest that it could be beneficial for unicellular such as yeast and trypanosomatid parasites, providing increased survival under stressful conditions. Among characterized trypanosomatids, Trypanosoma cruzi , brucei species from the genus Leishmania stand out due to their importance public health, infecting around 20 million people worldwide. The presence of aneuploidies T. was recently...
Sequences that have several copies in a genome, such as multicopy-gene families, mobile elements, and microsatellites, are among the most challenging genomic segments to study. They frequently underestimated genome assemblies, hampering correct assessment of these important players evolution adaptation.
Control of human ascariasis, the most prevalent neglected tropical disease globally affecting 450 million people, mostly relies on mass drug administration anthelmintics. However, chemotherapy alone is not efficient due to high re-infection rate for people who live in endemic area. The development a vaccine that reduces intensity infection and maintains lower morbidity should be primary target control. Previously, our group demonstrated immunization with crude Ascaris antigens mice induced...
Peptide-based vaccines have demonstrated to be an important way induce long-lived immune responses and, therefore, a promising strategy in the rational of vaccine development. As malaria, among classic targets, Apical membrane antigen (AMA-1) was proven B cell epitopes that can specific response hence, became key players for approach. The peptides selection carried out using bioinformatic approach based on Hidden Markov Models profiles known antigens and propensity scale methods...
Abstract Background Components of the antioxidant defense system in Trypanosoma cruzi are potential targets for new drug development. Superoxide dismutases (SODs) constitute key components systems, removing excess superoxide anions by converting them into oxygen and hydrogen peroxide. The main goal present study was to investigate genes coding iron dismutase (FeSOD) T. strains from an evolutionary perspective. Methods In this study, molecular biology methods phylogenetic studies were...
Abstract Background Aneuploidy is widely observed in both unicellular and multicellular eukaryotes, usually associated with adaptation to stress conditions. Chromosomal duplication stability a tradeoff between the fitness cost of having unbalanced gene copies potential gained from increased dosage specific advantageous genes. Trypanosomatids, family protozoans which include species that cause neglected tropical diseases, are relevant group study aneuploidies, as their life cycle has several...
Objetivo: Esta validação visou transferir o processamento de um painel 221 genes relacionados ao câncer germinativo para sequenciador DNBSEQ-G400 (MGI Tech Co), avaliando a quantidade e qualidade dos dados NGS gerados concordância na chamada variantes, em comparação com procedimento previamente validado no NextSeq-500 (Illumina, Inc). Método: O DNA do sangue 12 voluntários adultos foi submetido preparo bibliotecas à captura multicâncer foco desta validação, utilizando xGEN Library Prep and...
Objetivo: Este estudo investiga as variantes genéticas no gene G6PD Brasil, focando na análise de sua distribuição entre homens e mulheres utilizando dados laboratoriais. O objetivo principal é estimar a frequência indivíduos afetados portadores associadas à deficiência população brasileira. Método: Conduzido janeiro 2019 2024, este retrospectivo envolveu um banco laboratorial, todos anonimizados conforme Lei Geral Proteção Dados (LGPD). Incluiu-se uma amostra submetidos triagem genética...
Objetivo: Esta validação visou transferir o processamento de um exoma clínico 4700 genes relacionados a desordens hereditárias para sequenciador DNBSEQ-G400 (MGI Tech Co.), avaliando quantidade e qualidade dos dados NGS gerados concordância na chamada variantes, em comparação com procedimento previamente validado no NextSeq-500 (Illumina, Inc.). Método: O DNA da saliva 12 voluntários foi submetido ao preparo bibliotecas à captura contidos Inherited Disease Panel, utilizando xGEN Library Prep...
Visceral leishmaniasis is an important global health problem with estimated of 50,000 to 90,000 new cases per year. VL the most serious form as it can be fatal in 95% if remains untreated. a particularly acute Brazil which contributed 97% all reported 2020 Americas. In this country, affects mainly poorest people both urban and rural areas continues have high mortality rate around 8.15%. Here, we performed temporal parasite population study using whole genome sequence data from set 34 canine...
We report the sequencing and assembly of PH8 strain Leishmania amazonensis one etiological agents leishmaniasis. After combining data from long Pacbio reads, short Illumina reads synteny with mexicana genome, sequence 34 chromosomes 8317 annotated genes was generated. Multigene families encoding three virulence factors, A2, amastins GP63 metalloproteases, were identified compared to their annotation in other species. As they have been recently recognized as factors essential for disease...
The draft genome of the parasite Leishmania braziliensis strain BA788, which was isolated from a patient Bahia state, Brazil, sequenced using Illumina paired-end technology. assembled is 33.5 Mb long and contains 7,603 genes. This will contribute to studies aimed at understanding pathogenesis caused by this strain.