The role played by different mammal species in the maintenance of Trypanosoma cruzi is not constant and varies time place. This study aimed to characterise importance domestic, wild peridomestic hosts transmission T. Tauá, state Ceará, Caatinga area, Brazil, with an emphasis on those environments colonised Triatoma brasiliensis. Direct parasitological examinations were performed insects mammals, serologic tests household outdoor mammals multiplex polymerase chain reaction was used mammals....

Abstract Chagas disease was described by Carlos Chagas, who first identified the parasite Trypanosoma cruzi from a 2-year-old girl called Berenice. Many T. sequencing projects based on short reads have demonstrated that genome assembly and downstream comparative analyses are extremely challenging in this species, given half of its is composed repetitive sequences. Here, we report de novo assemblies, annotation, Berenice strain using combination Illumina MinION long reads. Our work...

The 2030 Agenda for Sustainable Development is a plan of action people, planet and prosperity. Thousands years centuries colonisation have passed the precarious housing conditions, food insecurity, lack sanitation, limitation surveillance, health care programs climate change. Chagas disease continues to be public problem. control been successful in many countries reducing transmission by T. cruzi; but results variable. WHO makes recommendations prevention with aim eliminating as Climate...

Knowledge of triatomine food sources in different ecotopes enables the estimation T. cruzi transmission risk diverse environments, as well its dynamics dispersion and ecological niche. For Triatoma brasiliensis Caatinga, northeast Brazil, seasonal differences influence feeding eclecticism rates infection. The objective present study was to monitor characterize populations associated with wild domestic environments Caatinga Brazil. A cross-sectional based on a search for triatomines...

The ecoepidemiological situation in the State of Rio Grande do Norte, Brazil is characterized by frequent invasion and colonization domiciliary units (DUs) several triatomine species, with high rates natural infection Trypanosoma cruzi. METHODS: We evaluated possibility vector transmission T. cruzi based on records occurrence domiciled triatomines collected Secretariat for Public Health from 2005 to 2015. During this period, 67.7% (113/167) municipalities conducted at least one active search...

Triatoma sordida is a native South American species and the most frequently captured triatomine in artificial environments Brazil. Although considered secondary vector of Trypanosoma cruzi, it typically associated with low infection rates. To investigate its role an endemic area for Chagas disease northern Minas Gerais, Brazil, we employed multidimensional approach that combined capture data quantitative qualitative analyses T. cruzi. A total 1861 specimens were captured, which 1455 examined...

Triatomines are blood-sucking insects that transmit the causative agent of Chagas disease, Trypanosoma cruzi. Despite being recognized as a difficult task, correct taxonomic identification triatomine species is crucial for vector control in Latin America, where disease endemic. In this context, we have developed web and mobile tool based on PostgreSQL database to help healthcare technicians overcome difficulties identify vectors when technical expertise missing. The version makes use real...

Correct identification of triatomine bugs is crucial for Chagas disease surveillance, yet available taxonomic keys are outdated, incomplete, or both. Here we present TriatoDex, an Android app-based pictorial, annotated, polytomous key to the Triatominae. TriatoDex was developed using Studio and tested by 27 Brazilian users. Each user received a box with pinned, number-labeled, adult triatomines (33 species in total) asked identify each bug level. We used generalized linear mixed models (with...

We sequenced maxicircles from T . cruzi strains representative of the species evolutionary diversity by using long-read sequencing, which allowed us to uncollapse their repetitive regions, finding that real lengths range 35 50 kb. have a common architecture composed four regions: coding region (CR), AT-rich region, short (SR) and long repeats (LR). Distribution genes, both in order strand orientation are conserved, being main differences presence deletions affecting genes for NADH...

The objective of this study was to evaluate the feeding behavior Triatoma vitticeps through identification its food sources and characterization blood ingestion process. In addition, we aimed verify if saliva vector interferes with perception host during feedings by creating a nervous impulse. Here, demonstrated that T. reduces, gradually irreversibly, amplitude compound action potential fibre, which helps decrease insect host. precipitin reaction eclecticism vector, eight - most them found...

The northeastern semiarid region stands out in the Brazilian context regarding eco-epidemiology of Chagas disease, which Triatoma brasiliensis is main vector Trypanosoma cruzi. Persistent house invasion threatens relative levels progress achieved over previous decades. We conducted an intervention trial with a five-year follow-up to assess impacts residual spraying pyrethroid insecticides on infestation T. 18 rural villages (242 houses) located Tauá, Ceará. House infestations were assessed...

The genus Cavernicola Barber 1937 and the tribe Cavernicolini Usinger 1944 were originally described from a single species, C. pilosa 1937. description of lenti Barrett & Arias 1985 rendered inadequate certain diagnostic characteristics chosen for these taxa. These taxa are redescribed here based on morphological morphometric studies two species.

Taxonomic markers (head structure morphometry, isoenzymes and randon amplified polymorphism of DNA - RAPD) were used to understand the population dynamics Triatoma vitticeps, predominant triatomine species in Itanhomi district, using samples obtained from domestic, peridomiciliary sylvatic habitats. Morphometric analysis revealed sexual dimorphism within three although specimens could not be separated according habitat which they captured. Forty-two bands analyzed RAPD profiles generated...

Triatomines (Hemiptera, Reduviidae) are vectors of Trypanosoma cruzi, the causative agent Chagas disease, one most important vector-borne diseases in Latin America. This study compares environmental niche spaces Triatoma brasiliensis and melanica using ecological modelling reports findings on DNA barcoding wing geometric morphometrics as tools for identification these species. We compared geographic distribution species generalized linear models fitted to elevation current data land surface...

The purpose of this study was to determine the potential for infestation man-made environments by Triatoma vitticeps, correlating epidemiological importance species with that others present in Brazilian state Minas Gerais. In addition, climatic variables could help explain distribution and clarify its population dynamics were sought. This done carrying out a historical survey presence T. vitticeps artificial ecotopes, using data from Fundação Nacional de Saúde (FUNASA). Monthly records...

Triatoma costalimai Verano & Galvão, a little-known vector of Trypanosoma cruzi Chagas, occupies rocky environments in the Brazilian Cerrado and occasionally infests man-made habitats. Entomological surveillance records show that T. occurs fairly often inside around houses south-eastern Tocantins, Brazil, with 859 specimens reported 2005–2014. Most adults were caught indoors, breeding colonies found houses. was detected 13.7% 839 bugs. These data suggest can contribute to transmission human...

The multiplicity of epidemiological scenarios shown by Chagas Disease, derived from multiple transmission routes the aetiological agent, occurring on geo-ecobiosocial settings determines complexity disease and reveal difficulties for its control. From first description link between parasite, vector domestic habitat that Carlos made in 1909, American Trypanosomiasis has a dynamic increasing complexity. These changed with time geography because new understandings studies, policies change at...

Panstrongylus lutzi is generally restricted to the "caatinga" areas of north-eastern Brazil. Adult insects are frequently found in local houses, but colonies have not previously been registered statistics Control Programme Chagas Disease. In Ceará State, our study revealed increasing occurrence this species, usually with high infection rates for Trypanosoma cruzi, and always represented by adults that invaded artificial environment. We also nymphs peridomicile inside houses. silvatic...

We report the first known occurrence of Psammolestes tertius Lent & Jurberg, 1965 (Hemiptera, Reduviidae, Triatominae) in state Rio Grande do Norte, Brazil. In 2009 and 2017, 2 adult specimens, a female male respectively, were collected at Seridó Ecological Station, which is located municipality Serra Negra Norte. These records broadens geographical distribution P. Brazil biodiversity triatomines

INTRODUCTION: Knowledge of triatomine bloodmeal sources is essential for understanding vector-host interactions in Trypanosoma cruzi transmission cycles. Expensive commercial deoxyribonucleic acid (DNA) extraction kits are widely used identification. This study assessed the performance an inexpensive phenol-chloroform DNA protocol identification sources, comparing it with a commercially available kit. METHODS: Both methods were to obtain from intestinal contents Triatoma brasiliensis...

Biological collections are depositories of information on different species and contribute to the knowledge, protection, conservation maintenance biodiversity.A list triatomine currently included in Collection Chagas Disease Vectors (FIOCRUZ-COLVEC) was prepared from database made available by Reference Center Environmental Information.COLVEC curatorship houses 4,778 specimens triatomines, which 811 come other American countries (Argentina, Bolivia, Colombia, Costa Rica, United States...

Abstract We sequenced maxicircles from T. cruzi strains representative of the species evolutionary diversity by using long-read sequencing, which allowed us to uncollapse their repetitive regions, finding that real lengths range 35 50 kb. have a common architecture composed four regions: coding region (CR), AT-rich region, short (SR) and long repeats (LR). Distribution genes, both in order strand orientation are conserved, being main differences presence deletions affecting genes for NADH...

Introduction We attempted to supplement traditional insecticide spraying by treating peridomiciliar food sources with a powder formulation. Methods Two groups of houses were treated deltamethrin suspension concentrate (SC), one which had its primary peridomestic 2P. Results Triatoma brasiliensis was the most commonly captured triatomine. Birds, dogs and rodents major identified precipitin reaction; 554 domestic animals received treatment. A sharp reduction in infestation rates observed two...