Although the genome of Trypanosoma cruzi, causative agent Chagas disease, was first made available in 2005, with additional strains reported later, intrinsic complexity this parasite (the abundance repetitive sequences and genes organized tandem) has traditionally hindered high-quality assembly annotation. This also limits diverse types analyses that require high degrees precision. Long reads generated by third-generation sequencing technologies are particularly suitable to address...

In prokaryotes, GC levels range from 25% to 75%, and T opt ≈0 °C >100 °C. When all species are considered together, no correlation is found between the two variables. Correlations found, however, when Families of prokaryotes analysed. Indeed, comprising at least 10 were studied (a set 20 Families), positive correlations for 15 them. Furthermore, a comparative analysis by independent contrasts made within in order control phylogenetic non‐independence showed qualitatively equivalent...

The tumor suppressor TP53 gene is one of the most frequently mutated in different types human cancer. Particularly colorectal cancer (CRC), it believed that mutations play a role adenoma-carcinoma transition tumors during pathological process. In order to analyze expressed alleles CRC, we examined mRNA samples from 101 patients with sporadic CRC. Samples were divided two groups defined according whether they exhibit positive or negative P53 protein expression as detected by...

American trypanosomiasis is a chronic and endemic disease which affects millions of people. Trypanosoma cruzi, its causative agent, has life cycle that involves complex morphological functional transitions, as well variety environmental conditions. This requires tight regulation gene expression, achieved mainly by post-transcriptional regulation. In this work we conducted an RNAseq analysis the three major stages T. cruzi: amastigotes, epimastigotes trypomastigotes. allowed us to delineate...

Abstract Background The common liver fluke Fasciola hepatica is the agent of a zoonosis with significant economic consequences in livestock production worldwide, and increasing relevance to human health developing countries. Although flukicidal drugs are available, re-infection emerging resistance demanding new efficient inexpensive control strategies. Understanding molecular mechanisms underlying host-parasite interaction provide relevant clues this search, while enlightening physiological...

Trypanosoma vivax is the earliest branching African trypanosome. This crucial phylogenetic position makes T. a fascinating model to tackle fundamental questions concerning origin and evolution of several features that characterize trypanosomes, such as Variant Surface Glycoproteins (VSGs) upon which antibody clearing antigenic variation are based. Other like gene content trans-splicing patterns worth analyzing in this species for comparative purposes.We present RNA-seq analysis bloodstream...

Abstract Apiculate yeasts belonging to the genus Hanseniaspora are predominant on grapes and other fruits. While some species, such as uvarum, well known for their abundant presence in fruits, they generally characterized by detrimental effect fermentation quality because excessive production of acetic acid. However, species vineae is adapted currently considered an enhancer positive flavour sensory complexity foods. Since 2002, we have been isolating strains from this conducting winemaking...

The pampas deer, Ozotoceros bezoarticus, was, until the recent past, widespread in South America from 5°S to 41°S; however, it is now considered be one of most endangered American cervids because habitat destruction and fragmentation. A comparative craniometrical study using univariate multivariate analyses was performed determine whether populations deer show patterns sexual dimorphism geographic variation. We found that exhibit strong dimorphism. Although species showed high levels...

Members of the GP63 metalloprotease family play crucial roles in parasite-host interactions, immune evasion, and pathogenesis. Although it has been widely studied Leishmania spp., less is known about its function diversity Trypanosoma cruzi . This study focuses on characterizing complete repertoire sequences T. genome, refining gene annotations, exploring evolutionary dynamics that shape these proteins. Eleven groups were identified, which are sharply defined have a higher intra- than...

Genomewide analyses of distances between orthologous gene pairs from the ascidian species Ciona intestinalis and savignyi were compared with those vertebrates. Combining this data a detailed careful use vertebrate fossil records, we estimated time divergence two ascidians nearly 180 My. This estimation was obtained after correcting for different substitution rates found comparing several groups chordates; indeed determine here that on average evolve 50% faster than

We sequenced maxicircles from T . cruzi strains representative of the species evolutionary diversity by using long-read sequencing, which allowed us to uncollapse their repetitive regions, finding that real lengths range 35 50 kb. have a common architecture composed four regions: coding region (CR), AT-rich region, short (SR) and long repeats (LR). Distribution genes, both in order strand orientation are conserved, being main differences presence deletions affecting genes for NADH...

The influence of the environment on two congeneric fishes, Gillichthys mirabilis and seta, that live in Gulf California at temperatures 10-25 degrees C, up to 42-44 respectively, was addressed by analyzing their genomes. Compared with G. mirabilis, seta showed some striking features. Substitution rates mitochondrial genes were found be extremely fast, fact faster than noncoding control regions (D-loops), from which a divergence time less 0.66-0.75 Mya could estimated. In nuclear genome, 1)...

Abstract In this work we analyze the variability in substitution rates GP63 gene from Leishmania. By using a sliding window to estimate along gene, found that rate of synonymous substitutions is highly correlated with both amino acid and codon bias. Furthermore, show comparisons involving genes represent independent phylogenetic lines yield very similar divergence/conservation patterns, thus suggesting deterministic forces (i.e., nonstochastic such as selection) generated these patterns. We...

The genus Cynolebias (Cyprinodontiformes: Rivulidae) is a locally endemic and speciose group of Neotropical fishes with an annual life cycle. Members the vary greatly in morphology behaviour, extensive interspecific karyotypic divergence has been documented among species from Uruguay, Argentina Rio Grande do Sul, Brazil. We present molecular systematic hypothesis relationships between these based on phylogenetic analysis combined dataset containing 1825 base pairs DNA sequence, representing...