A5045907511- Malaria Research and Control
- Mosquito-borne diseases and control
- Complement system in diseases
- Vector-borne infectious diseases
- Escherichia coli research studies
- Viral gastroenteritis research and epidemiology
- HIV-related health complications and treatments
- Neonatal Health and Biochemistry
- Blood groups and transfusion
- Salmonella and Campylobacter epidemiology
- Hemoglobinopathies and Related Disorders
- COVID-19 Clinical Research Studies
- HIV Research and Treatment
- HIV/AIDS Research and Interventions
- Antibiotic Resistance in Bacteria
- Long-Term Effects of COVID-19
- Microbial Metabolism and Applications
- Immune Response and Inflammation
- Toxin Mechanisms and Immunotoxins
- Biosensors and Analytical Detection
- Aquaculture disease management and microbiota
- Bacteriophages and microbial interactions
- Advanced Biosensing Techniques and Applications
- Child Nutrition and Water Access
- Pneumocystis jirovecii pneumonia detection and treatment
Fundação Oswaldo Cruz
2015-2025
Universidade Federal do Amazonas
2008-2025
Fundação de Medicina Tropical
2020-2024
Leoni (Germany)
2014-2024
Universidade do Estado do Rio de Janeiro
2024
Amazon (United States)
2021-2023
National Statistical Institute of Portugal
2023
Instituto de Fisiología Vegetal
2006
Faculdade de Medicina do ABC
2006
Institut de Neurobiologie de la Méditerranée
2003
Background.Plasmodium falciparum and Plasmodium vivax are responsible for most of the global burden malaria. Although accentuated pathogenicity P. occurs because sequestration mature erythrocytic forms in microvasculature, this phenomenon has not yet been noted vivax. The increasing number severe manifestations infections, similar to those observed malaria, suggests that key pathogenic mechanisms (eg, cytoadherence) might be shared by 2 parasites. Methods. Mature vivax–infected erythrocytes...
Malaria-induced sepsis is associated with an intense proinflammatory cytokinemia for which the underlying mechanisms are poorly understood. It has been demonstrated that experimental infection of humans Plasmodium falciparum primes Toll-like receptor (TLR)-mediated responses. Nevertheless, relevance this phenomenon during natural and, more importantly, by malaria mediates TLR hyperresponsiveness unclear. Here we show responses boosted in febrile patients P. . Microarray analyses...
ABSTRACT Violacein is a violet pigment extracted from the gram-negative bacterium Chromobacterium violaceum . It presents bactericidal, tumoricidal, trypanocidal, and antileishmanial activities. We show that micromolar concentrations efficiently killed chloroquine-sensitive -resistant Plasmodium falciparum strains in vitro; inhibited parasitemia vivo, even after parasite establishment; protected chabaudi -infected mice lethal challenge.
Background Although thrombocytopenia is a hematological disorder commonly reported in malarial patients, its mechanisms are still poorly understood, with only few studies focusing on the role of platelets phagocytosis. Methods and Findings Thirty-five malaria vivax patients eight healthy volunteers (HV) were enrolled study. Among (<150,000 platelets/µL) was found 62.9% (22/35). Mean platelet volume (MPV) higher thrombocytopenic as compared to non- (p = 0.017) negative correlation between...
In the present study, 470 children less than 72 months of age and presenting acute diarrhea were examined to identify associated enteropathogenic agents. Viruses pathogens most frequently found in stools infants with diarrhea, including 111 cases rotavirus (23.6% total cases) 30 adenovirus (6.3%). The second group was diarrheogenic Escherichia coli (86 cases, 18.2%), followed by Salmonella sp (44 9.3%) Shigella (24 5.1%). Using PCR technique differentiate pathogenic categories E. coli, it...
Shigellosis is a global human health problem and the incidence highest among children. In present work, main Shigella virulence genes was examined by PCR compared to symptoms of pediatric shigellosis. Thirty isolates were identified from an etiologic study at which 1,339 children ranging 0–10 years old enrolled. S. flexneri most frequent species reaching 60.0% isolates, 22.2% sonnei , 6.6% both dysenteriae boydii . All infected had diarrhea, but not all accompanied others bacillary...
There is now a growing body of evidence that challenges the current view Plasmodium vivax-infected erythrocyte (Pv-iE) are unable to sequester. Here we used ex vivo adhesion assays with Pv-iE before and after maturation demonstrate higher binding potential schizonts compared other asexual stages. These experimental results correlated our observations in panel 50 vivax malaria patients where were completely absent 27 isolates, few observed remaining patients. prompt paradigm shift P. biology...
Multi-drug resistant forms of Pseudomonas aeruginosa (MDRPA) are a major source nosocomial infections and when discharged into streams rivers from hospital wastewater treatment plants (HWWTP) they known to be able persist for extended periods. In the city Manaus (Western Brazilian Amazon), effluent three HWWTPs feed urban Mindu stream which crosses its rainforest before draining Rio Negro. The is routinely used by residents bathing cleaning (of clothes as well domestic utensils) and, during...
Malaria is a parasitic disease of great relevance in global public health. The development new sensitive and specific diagnostic high-throughput methods remains challenge the eradication this disease. In study, we developed flow cytometry test using latex microbeads polyclonal antibodies obtained from rabbits mice for detection P. vivax lactate dehydrogenase (PvLDH) antigen. We processed 50 samples Brazilian patients diagnosed with malaria caused by 40 healthy individuals. assay presented...
Despite progress in healthcare for people living with HIV/AIDS (PLWHA), many still present advanced HIV, thus increasing their risk of death. Late initiation treatment and poor adherence to antiretroviral therapy (ART) are key contributing factors. This study aimed evaluate cytokines as mortality predictors among hospitalized PLWHA. It assessed the death between ART-naïve ART-non-adherent PLWHA HIV quantified immunological markers post-mortem samples determine influence irregular ART use. A...
ABSTRACT Progress towards the development of a malaria vaccine against Plasmodium vivax , most widely distributed human parasite, will require better understanding immune responses that confer clinical protection to patients in regions where is endemic. The occurrence P. Brazil was first reported among residents riverine community Portuchuelo, Rondônia, western Amazon. We thus analyzed sera from this same population determine if naturally acquired humoral merozoite surface protein 1 PvMSP1,...
Plasmodium vivax is the causative agent of human malaria large geographic distribution, with 35 million cases annually. In Brazil, it most prevalent species, being responsible by around 70 % cases. A cross-sectional study was performed in Manaus (Amazonas, Brazil), including 36 adult patients primary malaria, 19 recurrent and 20 endemic controls. The ex vivo phenotypic features circulating leukocyte subsets (CD4+ T-cells, CD8+ NK, NKT, B, B1 Treg cells) as well plasmatic cytokine profile...
Introduction: Despite the efficacy of COVID-19, search for improvements in management severe/critical cases continues to be important. The aim is demonstrate kinetics 4 serological markers patients with COVID-19 who evolved hypoxemia. Methods: From June December 2020, Health Secretariat Rondônia State, Brazil, established a home medical care service team (HMCS) that provided clinical follow-up health professionals and military personnel COVID-19. laboratory monitoring was individualized at...
Abstract Background Plasmodium vivax has the potential to infect 2.85 billion individuals worldwide. Nevertheless, limited number of studies investigating immune status living in malaria-endemic areas, as well lack reports serological markers associated with clinical protection, hampered development vaccines for P. . It was previously demonstrated that naturally total IgG against N-terminus merozoite surface protein 1 (Pv-MSP1) reduced risk malarial infection. Methods Immune response Pv-MSP1...
Abstract Objective To investigate risk factors associated with the acquisition of antibodies against Plasmodium vivax Duffy binding protein (PvDBP) – a leading malaria vaccine candidate in well‐consolidated agricultural settlement Brazilian Amazon Region and to determine sequence diversity PvDBP ligand domain (DBP II ) within local parasite population. Methods Demographic, epidemiological clinical data were collected from 541 volunteers using structured questionnaire. Malaria parasites...
Background . Recent studies have shown that the inflammatory process, including biomarker production, and intense activation of innate immune responses are greater in malaria caused by Plasmodium vivax than other species. Here, we examined levels serum biomarkers their interaction during acute malaria. Material Methods Blood samples were collected from P. -infected patients at admission healthy donors. Levels measured Cytometric Bead Assay or ELISA. Results infection triggered production...
Background: Loss to follow-up (LTFU) among People Living with HIV (PLHIV) is a growing reality in Health system and can occur at any stage of care PLHIV. In LTFU situations, it result the deterioration health status quality life PLHIV, which also impacts prevalence transmission compromises continuous cascade goals (95-95-95) towards elimination HIV/AIDS 2030, created by UNAIDS.Method: This global systematic review investigated factors associated adult PLHIV (≥18 years) using Preferred...
Acute gastroenteritis is one of the most common diseases in humans worldwide. Viral a global problem infants and young children. In this study incidence diarrhea was assessed 877 hospitalized children under five years old, over period 24 months distributed 470 cases 407 age-matched group with other pathologies, as control group. Two antigen detection techniques based on enzyme immunoassay (EIA) latex particles were used for rotavirus adenovirus. Rotavirus A major cause 23.6% cases, being 90%...
The incidence of Shigella spp. was assessed in 877 infants from the public hospital Rondônia (Western Amazon region, Brazil) where represents fourth cause diarrhea. Twenty-five isolates were identified: 18 flexneri, three sonnei, boydii and one dysenteriae. With exception S. dysenteriae, all isolated children with diarrhea acquired multiple antibiotic resistances. PCR detection ipa virulence genes invasion assays bloody fever (colitis) compared among 25 patients testing positive for...
Abstract Background Immunoassays for Plasmodium detection are, presently, most frequently based on monoclonal antibodies (MAbs); Polyclonal (PAbs), which are cheaper to develop and manufacture, much less used. In the present study we describe a sandwich ELISA assay is capable of detecting P. vivax Lactate Dehydrogenase (LDH) in clinical blood samples, without cross reacting with those infected falciparum . Methods Two recombinant proteins were produced from different regions LDH gene. then...
The Plasmodium vivax Duffy binding protein (PvDBP) and its erythrocytic receptor, the antigen receptor for chemokines (DARC), are involved in major P. erythrocyte invasion pathway. An open cohort study to analyze DARC genotypes their relationship PvDBP immune responses was carried out 620 volunteers an agricultural settlement of Brazilian Amazon. Three cross-sectional surveys were conducted at 6-month intervals, comprising 395, 410, 407 subjects, respectively. incidence rates infection 2.32...
Abstract Nano-emulsions are promising carriers for antigen delivery. Here, we evaluated the efficacy of a water-oil nano-emulsion containing concentrated, inactivated Clostridium novyi (C. novyi) type B supernatant culture (nano-iCnB) in protecting Swiss mice against lethal dose alpha toxin concentrated extract. Proteins were confirmed nano-iCnB and their stabilities determined according physical parameters such as Zeta Potential (ZP). Biochemical, hematological morphological appearance...