A5034504407- Research on Leishmaniasis Studies
- Trypanosoma species research and implications
- Malaria Research and Control
- Vector-borne infectious diseases
- Plant biochemistry and biosynthesis
- Mosquito-borne diseases and control
- Parasites and Host Interactions
- Vector-Borne Animal Diseases
- Natural product bioactivities and synthesis
- Complement system in diseases
- Leptospirosis research and findings
- Appendicitis Diagnosis and Management
- Abdominal vascular conditions and treatments
- Biochemical and Molecular Research
- Diagnosis and Treatment of Venous Diseases
- Abdominal Surgery and Complications
- Monoclonal and Polyclonal Antibodies Research
- Bacteriophages and microbial interactions
- HIV/AIDS drug development and treatment
- Bat Biology and Ecology Studies
- Viral Infections and Vectors
- Vitamin K Research Studies
- Insect symbiosis and bacterial influences
- Male Reproductive Health Studies
- Antioxidant Activity and Oxidative Stress
Universidade de Santo Amaro
2019-2022
Universidade de São Paulo
2001-2018
Universidade Federal do ABC
2015-2017
Fundação Carlos Chagas
2003
Instituto de Biofísica Carlos Chagas Filho
2003
Pontifical Catholic University of Rio de Janeiro
2003
Fundação Oswaldo Cruz
2003
Universidade Federal de São Paulo
2003
Universidade de Brasília
2003
Carotenoids are widespread lipophilic pigments synthesized by all photosynthetic organisms and some nonphotosynthetic fungi bacteria. All carotenoids derived from the C40 isoprenoid precursor geranylgeranyl pyrophosphate, their chemical physical properties associated with light absorption, free radical scavenging, antioxidant activity. generally in well defined subcellular organelles, plastids, which also present phylum Apicomplexa, comprises a number of important human parasites, such as...
Herein, we show that intraerythrocytic stages of Plasmodium falciparum have an active pathway for biosynthesis menaquinone. Kinetic assays confirmed plasmodial menaquinone acts at least in the electron transport. Similarly to Escherichia coli , observed increased levels parasites kept under anaerobic conditions. Additionally, mycobacterial inhibitor synthesis Ro 48‐8071 also suppressed and growth parasites, although off‐targets may play a role this growth‐inhibitory effect. Due its absence...
Isoprenoids play important roles in all living organisms as components of structural cholesterol, steroid hormones mammals, carotenoids plants, and ubiquinones. Significant differences occur the length isoprenic side chains ubiquinone between different organisms, suggesting that enzymes are involved synthesis these chains. Whereas Plasmodium falciparum contain 7-9 units, 10-unit found humans. In a search for P. enzyme responsible biosynthesis attached to benzoquinone ring ubiquinones, we...
Environment influences the composition, distribution, and behavior of vectors mammalian hosts involved in transmission visceral leishmaniasis (VL), affecting epidemiology disease. In Brazil, urbanization process canine cases VL are indicators for local health authorities. This study aimed to investigate occurrence (CVL) Maranhão State, Brazil. Blood samples collected from 960 dogs six municipalities different ecosystems (Baixada Maranhense, Mangue, Mata dos Cocais, Amazônia, Cerrado,...
Abstract Background Leishmania infantum , the etiological agent of visceral leishmaniasis, is a neglected zoonosis that requires validation and standardization satisfactory diagnostic methodologies. Thus, aim present study was to evaluate effectiveness cathepsin L- like protease as target for making molecular diagnoses phylogenetic marker enabling understand intraspecies variations evolutionary history L. in Brazil. Methods We used 44 isolates . The gene fragments were amplified, sequenced,...
AbstractThe polymorphic merozoite surface protein-2 (MSP-2) of Plasmodium falciparum is a major malariavaccine candidate. In the present study, PCR and hybridization with allelic-specific probes were used to type Msp-2 gene from isolates hypo-endemic Brazil (N = 113), meso-endemic Vietnam 208) holo-endemic Tanzania 67). The typing methods designed group into dimorphic allelic families FC27 IC1 detect possible between-family recombination events. analysis was complemented by comparison 156...
ABSTRACT. The merozoite surface protein‐2 (MSP‐2) is a major vaccine candidate for the asexual blood stage of Plasmodium falciparum . MSP‐2 essentially dimorphic, and allelic families are named after representative isolates FC27 IC1. polymorphic central region contains immunodominant repeats, which vary in number, length, sequence within between families. We have examined antibody recognition repeat regions from both expressed as recombinant fusion peptides. results summarized follows. (1)...
Direct analysis of polyisoprenoid alcohols by electrospray ionization mass spectrometry (ESI-MS) often produces poor results requiring off-line time- and sample-consuming derivatization techniques. In this chapter, we describe a simple ESI-MS approach for the direct from biological samples. Lithium iodide is used to promote cationization intense formation [M+Li](+) adducts. Detection polyisoprenoids with determination can thus be performed high sensitivity (LOD near 100 pM), whereas...
Trypanosoma vivax Ziemann is a parasite that affects both wild and domestic ungulates transmitted mechanically via tabanids other blood-sucking insects in the Americas. A total of 621 blood samples from water buffaloes (Bubalus bubalis (Linnaeus) (Artiodactyla: Bovidae), 184 ectoparasite (Amblyomma cajennense (Fabricius) sensu stricto Rhipicephalus (Boophilus) microplus (Canestrini) (Acari: Ixodidae), Haematopinus tuberculatus (Burmeister) (Phthiraptera: Haematopinidae)) were obtained 60...
The National Park of Serra das Confusões (NPSC) is a protected area natural landscape located in Southern Piauí, Brazil, and it considered as one the largest most important areas Caatinga biome.The occurrences trypanosomatids from hemocultures on small mammals cultures intestinal contents triatomines were detected through molecular diagnoses blood samples, phylogenetic relationship analysis isolates parasites using trypanosome barcode (V7V8 SSUrDNA) realized.Only two Galea spixii (8.1%) six...
Background and Objectives: Canine visceral leishmaniasis affects dogs, the main urban reservoirs, which favor transmission expansion of this zoonotic disease in areas with high anthropization process human density. We investigated occurence Leishmania infatum based molecular diagnosis, phylogenetic analysis isolates obtained from dogs metropolitan region São Paulo. Methods: A total 201 were tested by parasitological diagnosis. Phylogenetic sequences SSUrDNA gGAPDH genes performed. Results:...
OBJECTIVE: Trypanosoma cruzi comprises highly heterogeneus populations classified within six Discrete Typing Unit (DTU´s) named Tc-I to Tc-VI ans TcBat. Evolutionary history of T. has a very strong association with their mammal hosts and some phylogenetic ecolobiologically studies suggest that ecotopes, vectors are factors determine the different lineages cruzi. METHODS: Herein we characterized isolates from synanthropic individuals Didelphis virginiana Rattus rattus captured in village...
ABSTRACT: Vector-borne diseases are currently one of the biggest public health concerns worldwide. Dogs, being closest companion animals to humans, considered main reservoir some these in urban environment. Therefore, study disease behavior dogs can help understand affecting human health. Serological and molecular diagnoses Babesia vogeli, Rangelia vitalli, Leishmania infantum, other trypanosomatids, were performed by immunochromatographic PCR assays, respectively, on a dog shelter located...
Vector-borne diseases are of great importance worldwide in human and veterinary medicine, as several parasites can cause disease multiple different hosts. In this work, we investigated the prevalence some vector-borne parasitic dogs Embu-Guacu, Sao Paulo, Brazil. Molecular diagnosis was performed by PCR using oligonucleotide markers specific for protozoans with significance both animal health, such Leishmania infantum , Rangelia vitalii Babesia vogeli . diagnostic results showed a high...
Trypanosoma cruzi relies on cysteine proteases (CPs) for efficient growth and survival inside the host.The activity of main parasite CP, cruzipain, has been directly or indirectly associated with intracellular amastigote survival, host cell invasion inflammation.Cruzipain is encoded by a large gene family, whose members present variable polymorphism are developmentally regulated.Studies cruzipain expression showed that it post-transcriptionally regulated, leading to increased levels CP in...
OBJECTIVE: Visceral leishmaniasis is caused by Leishmania infantum. In the state of São Paulo, this zoonosis expanding to urban areas and incidence human disease related canine infection. Herein, we performed a serological molecular diagnosis on 152 dogs (Canis familiaris) from Grajau Ilha do Bororé neighborhoods, in extreme south Paulo municipality, Brazil. METHODS: We observe prevalence L. infantum these animals that are identified as main reservoirs etiologic agent visceral leishmaniasis....
Introdução: A doença varicosa em membros inferiores é uma patologia de alta prevalência na população, e diversos fatores são relacionados com seu aparecimento evolução. Objetivo: avaliar a associação da alteração composição corporal associados à crônica vascular (DCV) varizes inferiores. Métodos: trata-se estudo transversal, prospectivo, pacientes submetidos cirurgia hospital referência, no período maio novembro 2018. Utilizou-se instrumento pesquisa semiestruturado questões que abordavam...
OBJECTIVE: To evaluate epidemiological, clinical, and prognostic profile of patients with acute vascular abdomen (AVA), framed as mesenteric ischemia (AMI) undergoing surgery. METHODS: Medical records AMI, who underwent surgery at Hospital Sancta Maggiore, São Paulo/SP, Brazil; between January 2017 to August 2021, were reviewed. For data analysis, the SPSS program version 18.0, independent Student t-test Chi-square tests used. RESULTS: Eighteen a median age 78 years selected. The main...