A5062465731- Peptidase Inhibition and Analysis
- Neuropeptides and Animal Physiology
- Protein Hydrolysis and Bioactive Peptides
- Protease and Inhibitor Mechanisms
- Venomous Animal Envenomation and Studies
- Signaling Pathways in Disease
- RNA and protein synthesis mechanisms
- Aquaculture Nutrition and Growth
- Phosphodiesterase function and regulation
- Circadian rhythm and melatonin
- Receptor Mechanisms and Signaling
- Trypanosoma species research and implications
- Antibiotic Resistance in Bacteria
- Enzyme Production and Characterization
- Ferrocene Chemistry and Applications
- Biochemical and Molecular Research
- Bacterial Genetics and Biotechnology
- Coccidia and coccidiosis research
- Neurobiology and Insect Physiology Research
- Biochemical and Structural Characterization
- Antimicrobial Peptides and Activities
- Cellular transport and secretion
- Proteoglycans and glycosaminoglycans research
- Glycosylation and Glycoproteins Research
- Adenosine and Purinergic Signaling
Universidade Federal de São Paulo
2016-2025
New York Medical College
2024-2025
Institute of Molecular Life Sciences
2025
University of Washington
2024
Westchester Medical Center
2024
Universidade Federal do Rio Grande
2008-2024
Seattle University
2023
Universidade de São Paulo
2001-2019
Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo
2011
Pingry School
2009
Recently we have shown that crotamine, a toxin from the South American rattlesnake Crotalus durissus terrificus venom, belongs to family of cell-penetrating peptides. Moreover, crotamine was demonstrated be marker centrioles, cell cycle, and actively proliferating cells. Herein show this at non-toxic concentrations is also capable binding electrostatically plasmid DNA forming DNA-peptide complexes whose stabilities overcome need for chemical conjugation carrying nucleic acids into...
Abstract Post-exercise hypotension is an important event for blood pressure regulation, especially in hypertensive individuals. Although post-exercise a well-known phenomenon, the mechanism responsible still unclear. The kallikrein-kinin system involved control, but its role has not yet been investigated. Thus, purpose of this study was to investigate involvement vasodilators bradykinin and des-Arg 9 -BK kallikrein activity promoted by 35 min cycle ergometer (CE) or circuit weight-training...
ABSTRACT Three Klebsiella pneumoniae clinical isolates demonstrating carbapenem resistance were recovered from different patients hospitalized at two medical centers in São Paulo, Brazil. Resistance to all β-lactams, quinolones, and some aminoglycosides was observed for these that susceptible polymyxin B. Carbapenem hydrolysis, which inhibited by clavulanic acid, K. belonged the same pulsed-field gel electrophoresis (PFGE) type a novel sequence (ST), ST1781 (clonal complex 442 [CC442]). A...
Our goal was to demonstrate the in vivo tumor specific accumulation of crotamine, a natural peptide from venom South American rattlesnake Crotalus durissus terrificus, which has been characterized by our group as cell penetrating with high specificity for actively proliferating cells and concentration-dependent cytotoxic effect. Crotamine cytotoxicity shown be dependent on disruption lysosomes subsequent activation intracellular proteases. In this work, we show that effect crotamine also...
An alkaline peptidase was purified from the viscera of silver mojarra (Diapterus rhombeus) in a three-step process: heat treatment, ammonium sulphate fractionation and molecular exclusion chromatography (Sephadex® G-75), with final specific activity 86-fold higher than enzyme extract yield 22.1%. The had an estimated mass 26.5kDa NH2-terminal amino acid sequence IVGGYECTMHSEAHE. Higher observed at pH 8.5 between 50 55°C. completely inactivated after 30min 55°C it significantly more stable...
We describe a method tailored to the in-cell selection of protease inhibitors. In this method, target is co-expressed with selective substrate, product which kills host cells. Therefore, can be applied identify potential inhibitors based on cell survival when inhibition occurs. The TEV was chosen for proof-of-concept experiment. genetically encoded substrate single polypeptide chain composed three parts: (1) ccdB protein, cause death it accumulates inside cell; (2) cleavage sequence that...
We report a systematic and detailed analysis of recombinant neurolysin (EC 3.4.24.16) specificity in parallel with thimet oligopeptidase (TOP, EC 3.4.24.15) using Bk sequence its C- N-terminal extensions as human kininogen motif for synthesis internally quenched fluorescent substrates. The influence the substrate size was investigated, longest peptide susceptible to TOP contains 17 amino acids. specificities both oligopeptidases sites P4 P3' were also characterized great detail seven series...
Recently, nuclear distribution element-like (NUDEL) has been implicated to play a role in lissencephaly and schizophrenia through interactions with the gene 1 (Lis1) disrupted-in-schizophrenia (DISC1) products, respectively. Interestingly, NUDEL is same protein as endooligopeptidase A (EOPA), thiol-activated peptidase involved conversion inactivation of number bioactive peptides. In this study, we have cloned EOPA from human brain confirmed that it equivalent NUDEL, leading us suggest single...
The oligopeptidase neurolysin (EC 3.4.24.16; Nln) was first identified in rat brain synaptic membranes and shown to ubiquitously participate the catabolism of bioactive peptides such as neurotensin bradykinin. Recently, it suggested that Nln reduction could improve insulin sensitivity. Here, we have KO mice increased glucose tolerance, sensitivity, gluconeogenesis. liver mRNA for several genes related Isotopic label semiquantitative peptidomic analysis suggests an increase specific...
Single-chain variable fragments (scFvs) are small-sized artificial constructs composed of the immunoglobulin heavy and light chain regions connected by a peptide linker. We have previously described an anti-fibroblast growth factor 2 (FGF2) G (IgG) monoclonal antibody (mAb), named 3F12E7, with notable antitumor potential revealed preclinical assays. FGF2 is known angiogenesis-associated molecule implicated in tumor progression. In this report, we describe recombinant scFv format for 3F12E7...
A trypsin was purified from pyloric caeca of pirarucu (Arapaima gigas). The effect metal ions and protease inhibitors on its activity physicochemical kinetic properties, as well N-terminal sequence, were determined. single band (28.0 kDa) observed by SDS–PAGE. Optimum pH temperature 9.0 65 °C, respectively. enzyme stable after incubation for 30 min in a wide range (6.0–11.5) at 55 °C. parameters Km, kcat kcat/Km 0.47 ± 0.042 mM, 1.33 s−1 2.82 mM−1, respectively, using BApNA substrate. This...
Abstract In schizophrenia (SCZ), higher angiotensin I-converting enzyme (ACE) levels have been reported in patient’s blood and cerebrospinal fluid (CSF). Hereby, we propose to explore whether the ACE activity are associated cognitive performance SCZ. Seventy-two patients with SCZ or schizoaffective disorder diagnosis, 69 healthy controls (HCs) underwent a battery parallel collection of peripheral samples measure activity. Significant were confirmed plasma compared HCs (Student’s t =−5.216; P...
Abstract Background Angiogenesis is a fundamental process that allows tumor growth by providing nutrients and oxygen to the cells. Beyond diffusion limit from capillary blood vessel, cells become apoptotic. results balance of pro- anti-angiogenic stimuli. Endogenous inhibitors regulate enzyme activities promote angiogenesis. Tumor may express pro-angiogenic factors hydrolytic enzymes but also kinin-degrading oligopeptidases which have been investigated. Results induced B16F10-Nex2 melanoma...
We explored furin substrate requirements in addition to the motif R-X-K/R-R using synthetic fluorescent resonance energy transfer (FRET) decapeptides. These decapeptides were derived from cleavage sites viral coat glycoproteins and human bacterial protein precursors. The hydrolysis by of most was activated K+ ion, whereas kosmotropic anions Hofmeister series inhibitors. analysis hydrolytic activity showed that its efficiency is highly dependent on particular combinations amino acids at...
An enzyme was purified from the pyloric caecum of tambaqui (Colossoma macropomum) through heat treatment, ammonium sulfate fractionation, Sephadex G-75 and p-aminobenzamidine-agarose affinity chromatography. The had a molecular mass 23.9 kDa, NH(2)-terminal amino acid sequence IVGGYECKAHSQPHVSLNI substrate specificity for arginine at P1, efficiently hydrolizing substrates with leucine lysine P2 serine P1'. Using z-FR-MCA, exhibited greatest activity pH 9.0 50 degrees C, whereas, BAPNA higher...
Bradykinin (BK) and its related peptides are widely distributed in venomous animals, including wasps. In fact, we have previously purified a novel BK-related peptide (BRP) named Cd-146 the threonine(6)-bradykinin (Thr(6)-BK) from venom of solitary wasp Cyphononyx fulvognathus. Further survey this same extract allowed structural characterization two other BRPs, here as fulvonin cyphokinin. Biochemical performed showed that although high primary structure similarity observed with BK, these not...
Phenothiazines (PTZ) are drugs widely used in the treatment of schizophrenia. Trifluoperazine, a piperazinic PTZ derivative, has been described as inhibitor mitochondrial permeability transition (MPT). We reported previously antioxidant activity thioridazine at relatively low concentrations associated to inhibition MPT (Brit. J. Pharmacol., 2002;136:136–142). In this study, it was investigated induction by derivatives higher than 10 μM focusing on molecular mechanism involved. promoted...
Posttraumatic knee stiffness is a very debilitating condition. Judet's quadricepsplasty technique has been used for more than 50 years. However, few reports of results exist in the literature.We report 45 cases posttraumatic arthrofibrosis treated with quadricepsplasty. The procedure were analyzed by measuring degrees flexion operated knees at different time points (before, immediately after, and late postoperatively).The degree increased from 33.6 (range, 5–80 degrees) preoperatively to 105...