A5101457656- Nitric Oxide and Endothelin Effects
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Cardiac Ischemia and Reperfusion
- Cancer, Hypoxia, and Metabolism
- Anesthesia and Neurotoxicity Research
- Redox biology and oxidative stress
- Glutathione Transferases and Polymorphisms
- Protein Kinase Regulation and GTPase Signaling
- Adenosine and Purinergic Signaling
- Adipokines, Inflammation, and Metabolic Diseases
- Sulfur Compounds in Biology
- Liver Disease and Transplantation
- Eicosanoids and Hypertension Pharmacology
- Synthesis and biological activity
- Protein Tyrosine Phosphatases
- Free Radicals and Antioxidants
- Cell Adhesion Molecules Research
- Organ Transplantation Techniques and Outcomes
- Renin-Angiotensin System Studies
- MicroRNA in disease regulation
- Immune cells in cancer
- SARS-CoV-2 and COVID-19 Research
- Analytical Chemistry and Chromatography
- Photochemistry and Electron Transfer Studies
- Retinoids in leukemia and cellular processes
Universidade Federal de São Paulo
2013-2024
Ministry of Science, Technology and Innovation
2023
Universidade Federal do Ceará
2023
Secretaria da Saúde
2023
Avanti (United Kingdom)
2023
University of Lisbon
2021
Fundação de Apoio à Universidade Federal de São Paulo
2010-2019
Universidade Federal da Grande Dourados
2010
The Royal Free Hospital
2004
University College London
2004
The aim of the present study was to determine mechanisms involved in estrogen actions cultured rat Sertoli cells. RT-PCR detected transcripts for receptors ESR1 and ESR2 immature cells testis 15-, 28-, 120-day-old rats. expression confirmed by immunofluorescence Western blot. Immunohistochemistry with cryosections testes from adult rats revealed that is Sertoli, Leydig, some peritubular myoid cells, multiple cell types, including germ Treatment 17beta-estradiol (E(2)) induced a translocation...
The first page of this article is displayed as the abstract.
S-nitrosylation of Cys118 is a redox-based mechanism for Ras activation mediated by nitric oxide (NO) at the plasma membrane.Ras signaling pathway stimulation 50 and/or 100 μM S-nitrosoglutathione (GSNO) causes proliferation HeLa cells. Proliferation was not observed in cells overexpressing non-nitrosatable H-Ras(C118S). H-Ras(wt) containing spatiotemporal probe green fluorescent protein (GFP) fused to Ras-binding domain Raf-1 (GFP-RBD) incubated with GSNO stimulated rapid and transient...
Abstract Background Angiogenesis is a fundamental process that allows tumor growth by providing nutrients and oxygen to the cells. Beyond diffusion limit from capillary blood vessel, cells become apoptotic. results balance of pro- anti-angiogenic stimuli. Endogenous inhibitors regulate enzyme activities promote angiogenesis. Tumor may express pro-angiogenic factors hydrolytic enzymes but also kinin-degrading oligopeptidases which have been investigated. Results induced B16F10-Nex2 melanoma...
Thioredoxin (TRX-1) is a multifunctional protein that controls the redox status of other proteins. TRX-1 can be found in extracellular milieu, cytoplasm and nucleus, it has distinct functions each environment. Previously, we studied intracellular localization its relationship with activation p21Ras - ERK1/2 MAP Kinases signaling pathway. In situations where this pathway was activated by stress conditions evoked nitrosothiol, S-nitroso-N-acetylpenicillamine (SNAP), accumulated nuclear...
Much attention has been drawn to the pro-inflammatory condition that accompanies aging. This study compared parameters from non-stimulated neutrophils, obtained young (18-30 years old [y.o.]) and elderly (65-80 y.o.) human volunteers. Measured as an inflammatory marker, plasmatic concentration of hs-CRP was found higher in individuals. Non-stimulated neutrophil production ROS NO was, respectively, 38 29% for aged group. From adhesion molecules evaluated, only CD11b expression elevated...
We have previously shown that ARG2 expression was increased in most malignant thyroid tumors, but absent benign lesions and normal tissues. Small interfering RNA knockdown used to investigate the role of a carcinoma cell line. decreased eNOS as well eNOS-related genes (p21, Akt1, HIF-1, VEGF, CAV1). silencing changed tumor properties cancer cells promoting apoptosis reduced proliferation markers. These results, coupled with enhanced nitric oxide production elevated reactive oxygen species...
The role of NO in regulating the focal adhesion proteins, Src, FAK, p130 Cas, and PTP-α, was investigated. Fibroblasts expressing PTP-α (PTP-αWT cells), fibroblasts "knockout" for (PTP-α−/− "rescued" (PTP-α A5/3 cells) were stimulated with either S-nitroso-N-acetylpenicillamine (SNAP) or fetal bovine serum (FBS). FBS increased inducible synthase both cell lines. Activation Src mediated by SNAP occurred independent dephosphorylation Tyr527 PTP-α−/− cells. Both stimuli promoted activation...
As an obligatory intracellular parasite, Trypanosoma cruzi, the etiological agent of Chagas' disease, must invade and multiply within mammalian cells. Cytokeratin 18 (CK18) is among host molecules that have been suggested as a mediator important events during T. cruzi-host cell interaction. Based on possibility, we addressed whether RNA interference (RNAi)-mediated down regulation CK18 gene could interfere with parasite life cycle in vitro. HeLa cells transiently transfected CK18-RNAi had...
Paracoccidioides brasiliensis, a causative agent of paracoccidioidomycosis (PCM), should be able to adapt dramatic environmental changes inside the infected host after inhalation air-borne conidia and transition pathogenic yeasts. Proteins with antioxidant functions may protect fungal cells against reactive oxygen (ROS) nitrogen (RNS) species generated by phagocytic cells, thus acting as potential virulence factors. Ras GTPases are involved in stress responses, cell morphology,...