A5009851064- Fungal Infections and Studies
- Antifungal resistance and susceptibility
- Monoclonal and Polyclonal Antibodies Research
- Glycosylation and Glycoproteins Research
- Click Chemistry and Applications
- Nitric Oxide and Endothelin Effects
- Fungal and yeast genetics research
- Research on Leishmaniasis Studies
- Plant Pathogens and Fungal Diseases
- Heat shock proteins research
- Extracellular vesicles in disease
- Plant-Microbe Interactions and Immunity
- Redox biology and oxidative stress
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Enzyme Structure and Function
- Protein Kinase Regulation and GTPase Signaling
- Enzyme Catalysis and Immobilization
- Protein Tyrosine Phosphatases
- Trypanosoma species research and implications
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Chemical Synthesis and Analysis
- Studies on Chitinases and Chitosanases
- Glutathione Transferases and Polymorphisms
- Phytoplasmas and Hemiptera pathogens
- Renin-Angiotensin System Studies
Universidade Federal de São Paulo
2014-2024
Instituto de Ciências Farmacêuticas
2013-2024
Instituto de Ciências Ambientais, Químicas e Farmacêuticas
2013
Fundação de Apoio à Universidade Federal de São Paulo
2010
ABSTRACT Paracoccidioides brasiliensis , a thermodimorphic fungus, is the causative agent of prevalent systemic mycosis in Latin America, paracoccidioidomycosis. We present here survey expressed genes yeast pathogenic phase P. . obtained 13,490 sequence tags from both 5′ and 3′ ends. Clustering analysis yielded partial sequences 4,692 that were functionally classified by similarity to known genes. have identified several Candida albicans virulence pathogenicity homologues Furthermore, we...
ABSTRACT Paracoccidioides brasiliensis is a thermodimorphic fungus associated with paracoccidioidomycosis (PCM), systemic mycosis prevalent in South America. In humans, infection starts by inhalation of fungal propagules, which reach the pulmonary epithelium and transform into yeast parasitic form. Thus, mycelium-to-yeast transition particular interest because conversion to essential for infection. We have used P. biochip carrying sequences 4,692 genes from this monitor gene expression at...
S-nitrosylation of Cys118 is a redox-based mechanism for Ras activation mediated by nitric oxide (NO) at the plasma membrane.Ras signaling pathway stimulation 50 and/or 100 μM S-nitrosoglutathione (GSNO) causes proliferation HeLa cells. Proliferation was not observed in cells overexpressing non-nitrosatable H-Ras(C118S). H-Ras(wt) containing spatiotemporal probe green fluorescent protein (GFP) fused to Ras-binding domain Raf-1 (GFP-RBD) incubated with GSNO stimulated rapid and transient...
Phage-display peptide libraries have been widely used to identify specific peptides targeting in vivo tumor cells and the vasculature playing an important role discovery of antitumor bioactive peptides. In present work, we identified a new melanoma-homing peptide, (-CVNHPAFAC-), using C7C phage-display library directed developing syngeneic mice. Phage were able preferentially target melanoma vivo, with affinity about 50-fold greater than that normal tissue, respective synthesized displaced...
Thioredoxin (TRX-1) is a multifunctional protein that controls the redox status of other proteins. TRX-1 can be found in extracellular milieu, cytoplasm and nucleus, it has distinct functions each environment. Previously, we studied intracellular localization its relationship with activation p21Ras - ERK1/2 MAP Kinases signaling pathway. In situations where this pathway was activated by stress conditions evoked nitrosothiol, S-nitroso-N-acetylpenicillamine (SNAP), accumulated nuclear...
Leishmania spp. is the aetiologic agent of leishmaniasis, a disease endemic in several developing countries. The parasite expresses and secretes virulence factors that subvert macrophage function immune response. Extracellular vesicles (EVs) can carry molecules parasites show immunomodulatory effects on activation progression. In present work, we detected significantly higher expression lpg3 gp63 genes amazonensis promastigotes recovered after successive experimental infections (IVD-P)...
Few virulence factors have been identified for Paracoccidioides brasiliensis, the agent of paracoccidioidomycosis. In this study, we quantitatively evaluated protein composition P. brasiliensis in yeast phase using minimal and rich media to obtain a better understanding its gain new insights into pathogen adaptation strategies. This analysis was performed on two isolates Pb18 strain showing distinct infection profiles B10.A mice. Using liquid chromatography/tandem mass spectrometry...
Paracoccidioides brasiliensis and P. lutzii, thermally dimorphic fungi, are the causative agents of paracoccidioidomycosis (PCM). infection occurs when conidia or mycelium fragments inhaled by host, which causes cells to transition yeast form. The development disease requires inside host alveoli differentiate into in a temperature-dependent manner. We describe presence two-component signal transduction system brasiliensis, we investigated expression analysis hypothetical protein gene...
All extracellular forms of Trypanosoma cruzi, the causative agent Chagas disease, release vesicles (EVs) containing major surface molecules parasite. EV depends on several mechanisms (internal and external). However, most environmental conditions affecting this phenomenon are still unknown. In work, we evaluated under different stress their ability to be internalized by parasites. addition, investigated whether would affect immunomodulatory properties in preactivated bone marrow-derived...
ABSTRACT J-domain (DnaJ) proteins, of the Hsp40 family, are essential cofactors their cognate Hsp70 chaperones, besides acting as independent chaperones. In present study, we have demonstrated presence Mdj1, a mitochondrial DnaJ member, not only in mitochondria, where it is apparently sorted, but also cell wall Paracoccidioides brasiliensis , thermodimorphic pathogenic fungus. The molecule (PbMdj1) was localized to fungal yeast cells using both confocal and electron microscopy flow...
The role of NO in regulating the focal adhesion proteins, Src, FAK, p130 Cas, and PTP-α, was investigated. Fibroblasts expressing PTP-α (PTP-αWT cells), fibroblasts "knockout" for (PTP-α−/− "rescued" (PTP-α A5/3 cells) were stimulated with either S-nitroso-N-acetylpenicillamine (SNAP) or fetal bovine serum (FBS). FBS increased inducible synthase both cell lines. Activation Src mediated by SNAP occurred independent dephosphorylation Tyr527 PTP-α−/− cells. Both stimuli promoted activation...
The fungi Paracoccidioides brasiliensis and lutzii are the causative agents of paracoccidioidomycosis (PCM), a systemic mycosis endemic to Latin America. This fungus is considered facultative intracellular pathogen that able survive replicate inside macrophages. survival during infection depends on its adaptability various conditions, such as nitrosative/oxidative stress produced by host immune cells, particularly alveolar Currently, there little knowledge about spp. signaling pathways...
Paracoccidioides brasiliensis, a causative agent of paracoccidioidomycosis (PCM), should be able to adapt dramatic environmental changes inside the infected host after inhalation air-borne conidia and transition pathogenic yeasts. Proteins with antioxidant functions may protect fungal cells against reactive oxygen (ROS) nitrogen (RNS) species generated by phagocytic cells, thus acting as potential virulence factors. Ras GTPases are involved in stress responses, cell morphology,...
B-1 cells are a subtype of B with peculiar characteristics. These distinct from B-2 lymphocytes in their morphology, ontogeny, tissue distribution, and phenotypic functional features. can participate the immune response several ways, for example, by being recruited to inflammatory foci, producing large amounts IL-10 cytokine, differentiating into IgM-secreting or phagocytes. Nevertheless, role pathogenesis experimental leishmaniasis has not been fully elucidated. Here we evaluated Leishmania...