A5031703106- Plant pathogens and resistance mechanisms
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Plant Pathogenic Bacteria Studies
- Circadian rhythm and melatonin
- Soybean genetics and cultivation
- Tryptophan and brain disorders
- Pancreatitis Pathology and Treatment
- Genetics and Plant Breeding
- Photoreceptor and optogenetics research
- Agronomic Practices and Intercropping Systems
- Plant Pathogens and Fungal Diseases
- Plant-Microbe Interactions and Immunity
- Horticultural and Viticultural Research
- bioluminescence and chemiluminescence research
- Neuroinflammation and Neurodegeneration Mechanisms
- Adipokines, Inflammation, and Metabolic Diseases
- Legume Nitrogen Fixing Symbiosis
- Photosynthetic Processes and Mechanisms
- Cell Adhesion Molecules Research
- Gut microbiota and health
- Atherosclerosis and Cardiovascular Diseases
- Antioxidant Activity and Oxidative Stress
- Agricultural pest management studies
- Phytochemicals and Antioxidant Activities
Servicio Regional de Investigación y Desarrollo Agroalimentario
2015-2024
Universidade de São Paulo
2013-2022
Hospital Universitário da Universidade de São Paulo
2021
University of Milan
2017
Instituto Butantan
2015
Universidade Brasil
2011
Instituto de Ciências Farmacêuticas
1992-2010
Universidad de Oviedo
2006
Merck (Germany)
2005
Universidade Federal de São Paulo
2000
Summary Neutrophils and monocytes play a central role in host defence. The invading leucocytes are capable of synthesizing releasing variety proinflammatory mediators including cytokines. Given the importance cytokines progression chronic acute inflammatory processes, we aimed to ascertain whether release interleukin (IL)-8, IL-1β, tumour necrosis factor (TNF)-α IL-1ra neutrophils was modified diabetes. To this end, measured suspensions cell culture basal lipopolysaccharide (LPS)-stimulated...
Abstract Serum amyloid A (SAA), a classical acute-phase protein, is produced predominantly by hepatocytes in response to injury, infection, and inflammation. It has been shown that SAA primes leukocytes induces the expression release of proinflammatory cytokines. Here, we report NO production murine peritoneal macrophages. Using specific inhibitors, showed was dependent on inducible synthase thorough activation ERK1/2 p38 MAPKs. Moreover, activity decreased after proteolysis but not with...
Dry bean (Phaseolus vulgaris L.) is one of the most important pulses consumed in world. Total phenolic content, total flavonoid monomeric anthocyanin content and antioxidant capacity were determined, using ferric reducing power free radical scavenging activity, 255 lines grown under same environmental conditions. For all parameters analysed, there was a wide range variability, with differences always above order magnitude. Phenolic compounds beans coloured coats found to be more efficient...
Abstract Domesticated crops have been disseminated by humans over vast geographic areas. Common bean ( Phaseolus vulgaris L.) was introduced in Europe after 1492. Here, combining whole-genome profiling, metabolic fingerprinting and phenotypic characterisation, we show that the first common cultigens successfully into were of Andean origin, Francisco Pizarro’s expedition to northern Peru 1529. We reveal hybridisation, selection recombination shaped genomic diversity European parallel with...
Abstract: N 1 ‐acetyl‐ 2 ‐formyl‐5‐methoxykynuramine (AFMK) and ‐acetyl‐5‐methoxykynuramine (AMK), two melatonin catabolites, have been described as potent antioxidants. We aimed to follow the kinetics of AFMK AMK formation when is oxidized by phorbol myristate acetate (PMA) lipopolysaccharide (LPS)‐activated leukocytes. An HPLC‐based method was used for determination in neutrophil peripheral blood mononuclear cell cultures supernatants. Samples were separated isocratically on a C18...
Bean anthracnose is caused by the fungus Colletotrichum lindemuthianum (Sacc. & Magnus) Lams.- Scrib. Resistance to C. in common bean (Phaseolus vulgaris L.) generally follows a qualitative mode of inheritance. The pathogen shows extensive pathogenic variation and up 20 resistance loci (named Co-), conferring specific races, have been described. Anthracnose has investigated analyzing limited number isolates or races segregating populations. In this work, we analyzed response against eleven...
In legumes, pod shattering occurs when mature pods dehisce along the sutures, and detachment of valves promotes seed dispersal. Phaseolus vulgaris (L)., major locus qPD5.1-Pv for indehiscence was identified recently. We developed a BC4/F4 introgression line population narrowed down to 22.5 kb region. Here, gene expression parallel histological analysis dehiscent indehiscent an AtMYB26 orthologue as best candidate loss shattering, on genomic region ~11 downstream highest associated peak....
Abstract Background Common bean ( Phaseolus vulgaris L.) is an important legume species which can be consumed as immature pods and dry seeds after re-hydration cooking. Many genes QTL, epistatic interactions among them, condition pod morphological traits. However, not all them have been mapped or validated nor candidate proposed. We sought to investigate the genomic regions conditioning color characters through GWAS. Results Single multi-locus genome wide association analysis was used traits...
Myeloperoxidase uses hydrogen peroxide to oxidize numerous substrates hypohalous acids or reactive free radicals. Here we show that neutrophils melatonin N(1)-acetyl-N(2)-formyl-5-methoxykynuramine (AFMK) in a reaction is catalyzed by myeloperoxidase. Production of AFMK was highly dependent on superoxide but not peroxide. It did require hypochlorous acid, singlet oxygen, hydroxyl radical. Purified myeloperoxidase and superoxide-generating system oxidized dimer. The dimer would result from...
Abstract Over the past 20 y, hormone melatonin was found to be produced in extrapineal sites, including cells of immune system. Despite increasing data regarding biological effects on regulation system, effect this molecule T cell survival remains largely unknown. Activation-induced death plays a critical role maintenance homeostasis system by eliminating self-reactive or chronically stimulated cells. Because activated not only synthesize but also respond it, we investigated whether could...
Indoleamine 2,3‐dioxygenase (IDO) is an interferon‐γ (IFN‐γ)–induced tryptophan‐degrading enzyme, producing kynurenine (KYN) that participates in the mechanism of tumor immune tolerance. Thus, IDO inhibition has been considered a strategy for anticancer therapy. The aim this study was to identify whether metabolites originated from competitive routes tryptophan metabolism, such as serotonergic or N , ‐dimethyltryptamine (DMT) pathways, have inhibitory effects on recombinant human (rhIDO)...
Melanoma is the most aggressive form of skin cancer and until recently, it was extremely resistant to radio-, immuno-, chemotherapy. Despite latest success BRAF V600E-targeted therapies, responses are typically short lived relapse all but certain. Furthermore, a percentage (40%) melanoma cells wild type. Emerging evidence suggests role for normal host in occurrence drug resistance. In current study, we compared variety cell culture models with an organotypic incomplete model (the "dermal...
A common bean (Phaseolus vulgaris) diversity panel of 308 lines was established from local Spanish germplasm, as well old and elite cultivars mainly used for snap consumption. Most the landraces included derived core collection, so this can be considered to representative species. The characterized by 3099 single-nucleotide polymorphism markers obtained through genotyping-by-sequencing, which revealed a wide genetic low level redundant material within panel. Structure, cluster, principal...
Anthracnose caused by Colletotrichum lindemuthianum (Sacc. & Magnus) Lams.‐Scrib. is a major disease affecting common bean ( Phaseolus vulgaris L.) crops worldwide. Response to five C. isolates, classified as races 3, 6, 7, 38, and 73, were analyzed in 156 F 2:3 families derived from the cross between line SEL1308 cultivar Michigan Dark Red Kidney (MDRK). was resistant all races, while MDRK susceptible except for race 73. Segregation ratio response 3 7 indicated that single dominant...
A large variation in seed coat colors and phenolic metabolites is present common bean (Phaseolus vulgaris L.). The study of the relationships between color phenotype profile an important step elucidation gene network involved phenylpropanoid biosynthetic pathway. However, this relationship still poorly understood species.A genome-wide association (GWAS) was used to investigate genomic regions associated with synthesis 10 flavonoids (5 anthocyanins 5 flavonols) traits using a set 308 lines...
The acute phase response is a systemic reaction to inflammatory processes characterized by multiple physiological adaptations, including the hepatic synthesis of acute‐phase proteins. In humans, serum amyloid A (SAA) one most prominent these Despite huge increase levels SAA in inflammation, its biological role remains be elucidated, even though undoubtedly active neutrophils. previous study, we reported that induces release tumor necrosis factor‐α, interleukin (IL)‐1β and IL‐8 from human...