A5033120586- Cell death mechanisms and regulation
- Chronic Myeloid Leukemia Treatments
- Chronic Lymphocytic Leukemia Research
- Immune Response and Inflammation
- Immune Cell Function and Interaction
- Phagocytosis and Immune Regulation
- Acute Lymphoblastic Leukemia research
- Inflammasome and immune disorders
- DNA Repair Mechanisms
- Immunotherapy and Immune Responses
- RNA Interference and Gene Delivery
- interferon and immune responses
- Molecular Biology Techniques and Applications
- Glycosylation and Glycoproteins Research
- CRISPR and Genetic Engineering
- Retinoids in leukemia and cellular processes
- Cancer-related Molecular Pathways
- Light effects on plants
- Circadian rhythm and melatonin
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Synthesis and Characterization of Heterocyclic Compounds
- Mitochondrial Function and Pathology
- NF-κB Signaling Pathways
- Autophagy in Disease and Therapy
- Fungal Infections and Studies
Universidade de São Paulo
2015-2024
Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular
2013-2024
Instituto Nacional de Tecnologia
2018
Trinity College Dublin
2017
Hospital Universitário da Universidade de São Paulo
2003-2011
Goethe University Frankfurt
2011
Millennium Institute
2002-2008
Instituto Nacional do Câncer
2007
Pirelli (Italy)
2004-2005
Institute of Biomedical Science
2004
Shailaja Kasibhatla, Gustavo P. Amarante-Mendes, Deborah Finucane,Thomas Brunner, Ella Bossy-Wetzel and Douglas R. GreenThis protocol was adapted from “Apoptosis Assays,” Chapter 15, in Cells (eds. Spector etal.). Cold Spring Harbor Laboratory Press, Harbor, NY, USA, 1998. Thisthree-volume set is now out of print; however, some the microscopy methods wererepublished Basic Methods Microscopy, by David L. Robert D.Goldman.
Phosphatidylserine (PS), a lipid normally confined to the inner leaflet of plasma membrane, is exported outer membrane during apoptosis serve as trigger for recognition apoptotic cells by phagocytes. The mechanism PS export not known nor it clear whether nuclear changes that typify contribute in any way this event. Here, we demonstrate ligation CD95 (Fas/APO-1) molecule on Jurkat cytoplasts induces dramatic externalization similar observed intact cells. Apoptosis both and was associated with...
MHC class Ia-restricted CD8(+) T cells are important mediators of the adaptive immune response against infections caused by intracellular microorganisms. Whereas antigen-specific effector can clear infection pathogens, in some circumstances, is suboptimal and microorganisms survive, causing host death or chronic infection. Here, we explored cellular molecular mechanisms that could explain why cell-mediated immunity during with human protozoan parasite Trypanosoma cruzi not optimal. For...
Significance This study describes a unique form of cell death induced by cytosolic flagellin that does not require NLRC4 (nucleotide oligomerization domain-like receptor family, caspase activation recruitment domain domain-containing 4), ASC (apoptosis-associated speck-like protein containing domain), or caspase-1/11 but is regulated lysosomal cathepsins B and D. Inflammasome-independent characterized loss membrane integrity IL-1α secretion participates in the clearance Salmonella...
BCL-X mRNA alternative splicing generates pro-apoptotic BCL-XS or anti-apoptotic BCL-XL gene products and the mechanism that regulates splice shifting is incompletely understood. We identified characterized a long non-coding RNA (lncRNA) named INXS, transcribed from opposite genomic strand of BCL-X, was 5- to 9-fold less abundant in tumor cell lines kidney, liver, breast prostate kidney tissues compared with non-tumors. INXS an unspliced 1903 nt-long RNA, by polymerase II, 5′-capped, nuclear...
Abstract Over the past 20 y, hormone melatonin was found to be produced in extrapineal sites, including cells of immune system. Despite increasing data regarding biological effects on regulation system, effect this molecule T cell survival remains largely unknown. Activation-induced death plays a critical role maintenance homeostasis system by eliminating self-reactive or chronically stimulated cells. Because activated not only synthesize but also respond it, we investigated whether could...
Saliva is a key element of interaction between hematophagous mosquitoes and their vertebrate hosts. In addition to allowing successful blood meal by neutralizing or delaying hemostatic responses, the salivary cocktail also able modulate effector mechanisms host immune responses facilitating, in turn, transmission several types microorganisms. Understanding how mosquito uses its components circumvent immunity might help clarify such pathogens disease establishment.Flow cytometry was used...
We examined cell death in developing retinal tissue, following inhibition of protein synthesis, which kills undifferentiated post-mitotic cells. Ultrastructural features were found both apoptosis and autophagy. Only approximately half the degenerating cells either terminal dUTP nick-end labeling (TUNEL)-positive or reacted with antibodies specific for activated caspases-3 -9. Bongkrekic acid completely inhibited any appearance death, whereas inhibitors autophagy, caspases-9 -3, prevented...