A5069975798- Plant pathogens and resistance mechanisms
- Plant Pathogenic Bacteria Studies
- Genetics and Plant Breeding
- Wheat and Barley Genetics and Pathology
- Genetic Mapping and Diversity in Plants and Animals
- Legume Nitrogen Fixing Symbiosis
- Genetic diversity and population structure
- Soybean genetics and cultivation
- Agricultural pest management studies
- Botanical Studies and Applications
- Cynara cardunculus studies
- Genetic and Environmental Crop Studies
- Plant Reproductive Biology
- Plant Disease Resistance and Genetics
- Powdery Mildew Fungal Diseases
- Agronomic Practices and Intercropping Systems
- Plant Pathogens and Fungal Diseases
- Seed Germination and Physiology
- Bryophyte Studies and Records
- Phytoplasmas and Hemiptera pathogens
- Genomics and Phylogenetic Studies
- Advances in Cucurbitaceae Research
- Lichen and fungal ecology
- Postharvest Quality and Shelf Life Management
- Plant Pathogens and Resistance
University of Sassari
2015-2024
Centro di Ricerca per la Patologia Vegetale
2013
Universidad de Santiago de Chile
2001
Summary We have studied the nucleotide diversity of common bean, P haseolus vulgaris , which is characterized by two independent domestications in geographically distinct areas: Mesoamerica and Andes. This provides an important model, as domestication can be a replicate experiment. used data from five gene fragments large introns to analyse 214 accessions (102 wild 112 domesticated). The represent cross‐section entire geographical distribution . A reduction genetic both these pools was...
Using RNA sequencing technology and de novo transcriptome assembly, we compared representative sets of wild domesticated accessions common bean (Phaseolus vulgaris) from Mesoamerica. was extracted at the first true-leaf stage, assembly used to develop a reference transcriptome; final data set consists ∼190,000 single nucleotide polymorphisms 27,243 contigs in expressed genomic regions. A drastic reduction diversity (∼60%) is evident for form, with almost 50% that are polymorphic were brought...
The first breeding program in the world for durum wheat was conceived Italy early 1900s. Over decades, pressure exerted by natural and artificial selection could have progressively reduced genetic diversity of germplasm. In present study, a large panel Italian accessions that includes landraces, old modern cultivars subjected to genotyping using Illumina iSelect 15K SNP array. aim assess impact has had shaping exploit patterns between populations identify molecular signatures divergence...
Abstract Domesticated crops have been disseminated by humans over vast geographic areas. Common bean ( Phaseolus vulgaris L.) was introduced in Europe after 1492. Here, combining whole-genome profiling, metabolic fingerprinting and phenotypic characterisation, we show that the first common cultigens successfully into were of Andean origin, Francisco Pizarro’s expedition to northern Peru 1529. We reveal hybridisation, selection recombination shaped genomic diversity European parallel with...
Summary Here we studied the organization of genetic variation common bean ( Phaseolus vulgaris ) in its centres domestication. We used 131 single nucleotide polymorphisms to investigate 417 wild accessions and a representative sample 160 domesticated genotypes, including Mesoamerican Andean for total 577 accessions. By analysing spatial patterns bean, documented existence several groups occurrence variable degrees diversity Mesoamerica Andes. Moreover, using landscape genetics approach,...
Summary The complete or partial loss of shattering ability occurred independently during the domestication several crops. Therefore, study this trait can provide an understanding link between phenotypic and molecular convergent evolution. genetic dissection ‘pod shattering’ in Phaseolus vulgaris is achieved here using a population introgression lines next‐generation sequencing techniques. ‘occurrence’ indehiscent phenotype (indehiscent versus dehiscent) depends on major locus chromosome 5....
Seed shattering in crops is a key domestication trait due to its relevance for seed dispersal, yield and fundamental questions evolution (e.g. convergent evolution). Here, we focused on pod common bean (Phaseolus vulgaris L.), the most important legume crop human consuption world. With this main aim, developed methodological pipeline that comprises thorough characterization under field conditions, including also chemical composition histological analysis of valves. The was based assumption...
In legumes, pod shattering occurs when mature pods dehisce along the sutures, and detachment of valves promotes seed dispersal. Phaseolus vulgaris (L)., major locus qPD5.1-Pv for indehiscence was identified recently. We developed a BC4/F4 introgression line population narrowed down to 22.5 kb region. Here, gene expression parallel histological analysis dehiscent indehiscent an AtMYB26 orthologue as best candidate loss shattering, on genomic region ~11 downstream highest associated peak....
Landraces are heterogeneous plant varieties that reproduced by farmers as populations subject to both artificial and natural selection. distinguished due their specific traits, different often grow of the same landrace. We used simple sequence repeats (SSRs) analyse 12 barley landrace from Sardinia two collections spanning 10 years. analysed population structure, compared diversity landraces were collected at field level (population). a representative pool for comparisons effects gene flow...
Evolutionary studies that are aimed at defining the processes behind present level and organization of crop genetic diversity represent fundamental bases for biodiversity conservation use. A Mesoamerican origin common bean Phaseolus vulgaris was recently suggested through analysis nucleotide polymorphism nuclear level. Here, we have used chloroplast microsatellites to investigate bean, on basis specific characteristics these markers (no recombination, haploid genome, uniparental...
Abstract The common bean ( Phaseolus vulgaris L.) is a crucial legume crop and an ideal evolutionary model to study adaptive diversity in wild domesticated populations. Here, we present pan-genome based on five high-quality genomes whole-genome reads representing 339 genotypes. It reveals ~234 Mb of additional sequences containing 6,905 protein-coding genes missing from the reference, constituting 49% all presence/absence variants (PAVs). More non-synonymous mutations are found PAVs than...
Relatively few studies have extensively analysed the genetic diversity of runner bean through molecular markers. Here, we used six chloroplast microsatellites (cpSSRs) to investigate cytoplasmic 331 European domesticated accessions scarlet (Phaseolus coccineus L.), including botanical varieties albiflorus, bicolor and coccineus, a sample 49 wild from Mesoamerica. We further explored pattern landraces using 12 phenotypic traits on 262 individuals. For 158 accessions, studied relationships...
Crop species have been deeply affected by the domestication process, and there many efforts to identify selection signatures at genome level. This knowledge will help geneticists better understand evolution of organisms, same time, breeders implement successful breeding strategies. Here, we focused on in Mesoamerican gene pool Phaseolus vulgaris sequencing 49 fragments from a sample 45 P. wild domesticated accessions, as controls, two accessions each closely related coccineus dumosus. An...
Brazil is the largest consumer and third highest producer of common beans (Phaseolus vulgaris L.) worldwide. Since 1980s, commercial Carioca variety has been most consumed in Brazil, followed by Black Special beans. The present study evaluates genetic diversity population structure 185 Brazilian bean cultivars using 2827 high-quality single-nucleotide polymorphisms (SNPs). Andean allelic introgression Mesoamerican accessions was investigated, a panel tested an association mapping approach....
Abstract We examined the local adaptation patterns in a system comprising several interconnected heterogeneous plant populations from which of two phylogenetically closely related pathogens were also sampled. The host is Hordeum vulgare (cultivated barley); are Pyrenophora teres f. (net form) and maculata (spot form), causal agents barley net blotch. integrated approaches, comparison between population structures cross-inoculation test. demonstrated that with very similar niche...
Tomato (Solanum lycopersicum L.) is a widely used model plant species for dissecting out the genomic bases of complex traits to thus provide an optimal platform modern “-omics” studies and genome-guided breeding. Genome-wide association (GWAS) have become preferred approach screening large diverse populations many traits. Here, we present GWAS analysis collection 115 landraces 11 vintage cultivars. A total 26 conventional descriptors, 40 obtained by digital phenotyping, fruit content six...
By selecting for prostrate growth habit of the juvenile phase cycle, durum wheat cultivars could be developed with improved competitive ability against weeds, and better soil coverage to reduce water lost by evaporation. A panel 184 (Triticum turgidum subsp. durum) genotypes, previously genotyped DArT-seq markers, was used perform association mapping analysis prostrate/erect trait identify candidate genes. Phenotypic data plant were recorded during three consecutive growing seasons...