A5064463064- Legume Nitrogen Fixing Symbiosis
- Plant nutrient uptake and metabolism
- Agronomic Practices and Intercropping Systems
- Plant Pathogenic Bacteria Studies
- Nematode management and characterization studies
- Plant pathogens and resistance mechanisms
- Wastewater Treatment and Nitrogen Removal
- Insect and Pesticide Research
- Plant-Microbe Interactions and Immunity
- Plant Micronutrient Interactions and Effects
- Plant and animal studies
- Soybean genetics and cultivation
- Microbial Community Ecology and Physiology
- Cassava research and cyanide
- Metalloenzymes and iron-sulfur proteins
- Coastal wetland ecosystem dynamics
- Air Quality Monitoring and Forecasting
- Microbial Fuel Cells and Bioremediation
- Plant Virus Research Studies
- Agricultural pest management studies
- Plant Reproductive Biology
- Ammonia Synthesis and Nitrogen Reduction
- Indoor Air Quality and Microbial Exposure
- Peanut Plant Research Studies
- Soil Carbon and Nitrogen Dynamics
Instituto de Biotecnología y Biología Molecular
2013-2024
Consejo Nacional de Investigaciones Científicas y Técnicas
2013-2024
Universidad Nacional de La Plata
2009-2023
Michigan State University
2000-2004
Centre de Génétique Moléculaire
2001
GTx (United States)
2000
University of Alberta
1990
Bielefeld University
1984-1987
University of Sussex
1985
Legumes are the third largest family of angiosperms and second most important crop class. Legume genomes have been shaped by extensive large-scale gene duplications, including an approximately 58 million year old whole genome duplication shared legumes.
Modern civilization depends on only a few plant species for its nourishment. These crops were derived via several thousands of years human selection that transformed wild ancestors into high-yielding domesticated descendants. Among cultivated plants, common bean (Phaseolus vulgaris L.) is the most important grain legume. Yet, our understanding origins and concurrent shaping genome this crop limited. We sequenced genomes 29 accessions representing 12 Phaseolus species. Single nucleotide...
We describe stromatolites forming at an altitude of 3570 m the shore a volcanic lake Socompa, Argentinean Andes. The water site formation is alkaline, hypersaline, rich in inorganic nutrients, very arsenic, and warm (20–24°C) due to hydrothermal input. do not lithify, but form broad, rounded low-domed bioherms dominated by diatom frustules aragonite micro-crystals agglutinated extracellular substances. In comparison other modern stromatolites, they harbour atypical microbial community...
Summary A dataset of 87 020 nifH reads and 16 782 unique protein sequences obtained over 2 years from four locations across a gradient agricultural soil types in rgentina were analysed to provide detailed comprehensive picture the diversity, abundance responses N ‐fixing community relation differences chemistry practices. Phylogenetic analysis revealed an expected high proportion lphaproteobacteria , B etaproteobacteria D eltaproteobacteria mainly relatives radyrhizobium M ethylosinus /...
ABSTRACT The isolation of rhizobial strains which exhibit an intrinsic tolerance to acidic conditions has been reported and facilitated studies on the basic mechanisms underlying acid tolerance. Rhizobium tropici strain CIAT899 displays a high acidity therefore was used in this work study molecular basis bacterial responses other environmental stresses. We generated collection R. mutants affected using Tn 5-luxAB mutagenesis, one mutant (CIAT899-13T2), fails grow under conditions,...
Common beans ( Phaseolus vulgaris ) comprise three major geographic genetic pools, one in Mexico, Central America, and Colombia, another the southern Andes, a third Ecuador northern Peru. Species Rhizobium etli is predominant rhizobia found symbiotically associated with Americas. We have polymorphism common nodulation gene nod C among R. strains from wide range of geographical origins, which disclosed types. The different alleles American show varying predominance their regional...
Abstract Legume plants are able to interact symbiotically with soil bacteria form nitrogen-fixing root nodules. Although specific recognition between rhizobia and legume species has been extensively characterized, plant molecular determinants that govern the preferential colonization by different strains within a single rhizobium have received little attention. We found C subunit of heterotrimeric nuclear factor NF-Y from common bean (Phaseolus vulgaris) NF-YC1 plays key role in improved...
Abstract A C subunit of the heterotrimeric nuclear factor Y (NF-YC1) was shown to play a key role in nodule organogenesis and bacterial infection during nitrogen fixing symbiosis established between common bean (Phaseolus vulgaris) Rhizobium etli. To identify other proteins involved this process, we used yeast (Saccharomyces cerevisiae) two-hybrid system screen for NF-YC1-interacting proteins. One positive clones encodes member Phytochrome Signal Transduction1 subfamily GRAS (for Gibberellic...
ABSTRACT Rhizobia form a symbiotic relationship with plants of the legume family to produce nitrogen-fixing root nodules under nitrogen-limiting conditions. We have examined importance glutathione (GSH) during free-living growth and symbiosis Sinorhizobium meliloti . An S. mutant strain (SmgshA) which is unable synthesize GSH due gene disruption in gshA , encoding enzyme for first step biosynthesis GSH, was grow nonstress conditions, precluding any nodulation. In contrast, an (SmgshB) gshB...
Abstract The common bean ( Phaseolus vulgaris L.) is a crucial legume crop and an ideal evolutionary model to study adaptive diversity in wild domesticated populations. Here, we present pan-genome based on five high-quality genomes whole-genome reads representing 339 genotypes. It reveals ~234 Mb of additional sequences containing 6,905 protein-coding genes missing from the reference, constituting 49% all presence/absence variants (PAVs). More non-synonymous mutations are found PAVs than...
We describe the isolation and characterization of alfalfa-nodulating rhizobia from acid soils different locations in Central Argentina Uruguay. A collection 465 isolates was assembled, were characterized for tolerance. Growth tests revealed existence 15 acid-tolerant (AT) which able to grow at pH 5.0 formed nodules alfalfa with a low rate nitrogen fixation. Analysis those isolates, including partial sequencing genes encoding 16S rRNA genomic PCR-fingerprinting MBOREP1 BOXC1 primers,...
Abstract Legume plants are able to establish a symbiotic relationship with soil bacteria from the genus Rhizobium, leading formation of nitrogen-fixing root nodules. Successful nodulation requires both infection threads (ITs) in epidermis and activation cell division cortex form nodule primordium. This study describes characterization RabA2, common bean (Phaseolus vulgaris) cDNA previously isolated as differentially expressed hairs infected Rhizobium etli, which encodes protein highly...
Rhizobium meliloti 2011 DNA from pRmSL26, a plasmid which is known to carry genes involved in the early stages of nodulation, was used construct Tn5 mutations by site-directed mutagenesis. located within an 8.7 kilobase EcoRI fragment defined two adjacent loci encoding functions for nodulation (nod) and symbiotic N2 fixation (fix). We investigated organization regulation fix locus characteristics alfalfa nodules induced these Fix- mutants. By monitoring expression Escherichia coli minicells,...
In this survey, a total of 80 787 reads and 28 171 unique NifH protein sequences were retrieved from soil RNA. This dataset extends our knowledge about the structure diversity functional diazotrophic communities in agricultural soils Argentinean Pampas. Operational taxonomic unit (OTU)-based analyses showed that nifH phylotypes related to Geobacter Anaeromyxobacter (44.8%), Rhizobiales (29%), Cyanobacteria (16.7%), Verrucomicrobiales (8%) are key microbial components N2 fixation associated...
ABSTRACT A rapid procedure for the identification of Paenibacillus larvae subsp. , causal agent American foulbrood (AFB) disease honeybees ( Apis mellifera L.), based on PCR and restriction fragment analysis 16S rRNA genes (rDNA) is described. Eighty-six bacterial strains belonging to 39 species genera Bacillus Brevibacillus Virgibacillus were characterized. Amplified rDNA was digested with seven endonucleases. The combined data from enabled us distinguish 35 profiles. Cluster revealed that...
SUMMARYPaenibacillus larvae larvae, the causative agent of American foulbrood disease honey bee occurs throughout world and is found in all beekeeping areas Argentina. Microbiological analysis 394 samples obtained from hives Buenos Aires province (Argentina) three years sampling (1999–2001) yielded 219 positive cases (55.6%). The incidence P l. infected for 1999 was 68.1% (n = 160), 2000 47.1% 102), 46.2% 2001 132). mean values spore contamination three-year study showed a continuous...
In this paper, we examine the importance of glutathione in symbiosis, using a biosynthetic gshB mutant derived from Rhizobium tropici CIAT899, common bean (Phaseolus vulgaris) endosymbiont. Plants infected with strain presented delayed nodulation phenotype and reduction dry weight aerial part plants, suggesting diminished nitrogen-fixation activity. addition, bacterial expression was assayed wild-type nodules, during different steps nodulation, found to increase mature early senescent...
An essential gene for symbiotic nitrogen fixation (fixF) is located near the common nodulation region of Rhizobium meliloti. A DNA fragment carrying fixF was characterized by hybridization with Klebsiella pneumoniae nif and nucleotide sequence analysis. The found to be related K. nifN therefore renamed as R. meliloti gene. Upstream coding a second open reading frame identified putative polypeptide 110 amino acids (ORF110). By fragment-specific Tn5 mutagenesis it shown that ORF110 form an...
ABSTRACT A collection of rhizobial isolates from nodules wild beans, Phaseolus vulgaris var. aborigineus, found growing in virgin lands 17 geographically separate sites northwest Argentina was characterized on the basis host range, growth, hybridization to a nifH probe, analysis genes coding for 16S rRNA (16S rDNA), DNA fingerprinting, and plasmid profiles. Nodules field-collected bean plants were largely dominated by rhizobia carrying rDNA allele Rhizobium etli . similar prevalence R....