A5056793178- Plant nutrient uptake and metabolism
- Legume Nitrogen Fixing Symbiosis
- Plant Micronutrient Interactions and Effects
- Agronomic Practices and Intercropping Systems
- Plant Stress Responses and Tolerance
- Nematode management and characterization studies
- Endoplasmic Reticulum Stress and Disease
- Plant Molecular Biology Research
- Plant pathogens and resistance mechanisms
- Trace Elements in Health
- Growth and nutrition in plants
- Aluminum toxicity and tolerance in plants and animals
- RNA modifications and cancer
- Public Health and Environmental Issues
- Pancreatic function and diabetes
- Iron Metabolism and Disorders
- Autophagy in Disease and Therapy
- Plant-Microbe Interactions and Immunity
- Plant and soil sciences
- Agricultural and Food Production Studies
- RNA Research and Splicing
University of Lausanne
2020-2024
Instituto de Biotecnología y Biología Molecular
2014-2023
Consejo Nacional de Investigaciones Científicas y Técnicas
2014-2023
Universidad Nacional de La Plata
2014-2023
Centro Científico Tecnológico - La Plata
2014
Centro Científico Tecnológico - Tucumán
2014
Arabidopsis primary root growth response to phosphate (Pi) deficiency is mainly controlled by changes in apoplastic iron (Fe). Upon Pi deficiency, Fe deposition the apical meristem activates pathways leading arrest of maintenance and inhibition cell elongation. Here, we report that a member uncharacterized cytochrome b561 DOMON domain (CYBDOM) protein family, named CRR, promotes reduction an ascorbate-dependent manner controls deposition. Under low Pi, crr mutant shows enhanced associated...
Abstract A C subunit of the heterotrimeric nuclear factor Y (NF-YC1) was shown to play a key role in nodule organogenesis and bacterial infection during nitrogen fixing symbiosis established between common bean (Phaseolus vulgaris) Rhizobium etli. To identify other proteins involved this process, we used yeast (Saccharomyces cerevisiae) two-hybrid system screen for NF-YC1-interacting proteins. One positive clones encodes member Phytochrome Signal Transduction1 subfamily GRAS (for Gibberellic...
Transcription factors of the Nuclear Factor Y (NF-Y) family play essential functions in plant development and plasticity, including formation lateral root organs such as symbiotic nodules. NF-Ys mediate transcriptional responses by acting heterotrimers composed three subunits, NF-YA, NF-YB, NF-YC, which plants are encoded relatively large gene families. We have previously shown that, Phaseolus vulgaris × Rhizobium etli interaction, PvNF-YC1 subunit is involved not only nodules, but also...
Abstract Legumes play an important role in the soil nitrogen availability via symbiotic fixation (SNF). Phosphate (Pi) deficiency severely impacts SNF because of high Pi requirement symbiosis. Whereas PHT1 transporters are involved uptake into nodules, it is unknown how transferred from plant infected cells to nitrogen-fixing bacteroids. We hypothesized that Medicago truncatula genes homologous Arabidopsis PHO1, encoding a vascular apoplastic exporter, transfer Among seven MtPHO1 present M....
Abstract Legume plants establish a nitrogen-fixing symbiosis with soil bacteria known as rhizobia. Compatibility between legumes and rhizobia is determined at species-specific level, but variations in the outcome of symbiotic process are also influenced by capacity plant to discriminate select specific strains that better partners. We compared transcriptional response two genetically diverse accessions Phaseolus vulgaris from Mesoamerica South Andes Rhizobium etli exhibit variable degrees...
Abstract Accumulation of incompletely folded proteins in the endoplasmic reticulum (ER) leads to ER stress, activates protein degradation pathways, and upregulates genes involved folding. This process is known as unfolded response (UPR). The role folding plant responses nutrient deficiencies unclear. We analyzed Arabidopsis (Arabidopsis thaliana) mutants affected quality control established that both CALNEXIN (CNX) function primary root phosphate (Pi) deficiency. CNX1 CNX2 are homologous...
SUMMARY Alternative transcription start sites (TSS) are widespread in eukaryotes and can alter the 5′ UTR length coding potential of transcripts. Here we show that inorganic phosphate (Pi) availability regulates usage several alternative TSS Arabidopsis ( thaliana ). In comparison to phytohormone treatment, Pi had a pronounced specific effect on many TSS. By combining short‐read RNA sequencing with long‐read full‐length mRNAs, identified set 45 genes showing under deficiency. affected...
Abstract Phosphorous and nitrogen are the main nutrients limiting plant growth in natural agricultural systems. Plants typically uptake phosphorous form of inorganic phosphate, whereas sources nitrate ammonium. Roots show a high degree developmental plasticity response to nutritional stresses, directly influencing fitness stress resilience. In Arabidopsis thaliana , phosphate deficiency levels ammonium media triggers strong inhibition primary root growth. This is initiated by accumulation...
Abstract Iron (Fe) and phosphate (Pi) are essential nutrients for plant growth. Several interactions between Fe Pi homeostasis have been described, such as the Fe-dependent inhibition of primary root growth under deficiency. This response involves formation apoplastic +3 -malate complexes in meristem which implicates oxidation +2 by LPR1 ferroxidase. However, how is reduced generated /Fe ratio regulated unknown. Here, we identified a gene Arabidopsis thaliana , named CRR that implicated...
Heterotrimeric Nuclear Factor Y (NF-Y) transcription factors are key regulators of the symbiotic program that controls rhizobial infection and nodule organogenesis. Using a yeast two-hybrid screening, we identified putative protein kinase Phaseolus vulgaris interacts with C subunit NF-Y complex. Physical interaction between NF-YC1 Interacting Protein Kinase (NIPK) occurs in cytoplasm plasma membrane. Only one three canonical amino acids predicted to be required for catalytic activity is...
Abstract Accumulation of incompletely folded proteins in the endoplasmic reticulum (ER) leads to ER stress, activates protein degradation pathways, and upregulates genes involved folding (Unfolded Protein Response; UPR). stress has been associated with abiotic conditions that affect folding, including salt stress. However, role plant responses nutrient deficiencies is unclear. We analyzed several Arabidopsis thaliana mutants affected quality control established both CALNEXIN ( CNX ) function...
Abstract Alternative transcription start sites (TSSs) are widespread in eukaryotes. In plants, light, development and tissue regulate selective usage of several TSSs, producing transcripts with distinct 5′UTR as well shorter protein isoforms subcellular localization or activity. However, the function non-coding generated by alternative TSSs is still largely unknown. We show that phosphate availability regulates numerous including a TSS ( ALT ECT4 ) associated , encoding N 6 -methyladenosine...