A5080174209- Legume Nitrogen Fixing Symbiosis
- Plant nutrient uptake and metabolism
- Nematode management and characterization studies
- Agronomic Practices and Intercropping Systems
- Plant Stress Responses and Tolerance
- Insect Resistance and Genetics
- Microbial Community Ecology and Physiology
- Microbial Fuel Cells and Bioremediation
- Signaling Pathways in Disease
- Plant Parasitism and Resistance
- Soybean genetics and cultivation
- Nitrogen and Sulfur Effects on Brassica
- Plant Micronutrient Interactions and Effects
- Plant responses to water stress
- Wastewater Treatment and Nitrogen Removal
- Metalloenzymes and iron-sulfur proteins
- Plant tissue culture and regeneration
- Plant Molecular Biology Research
- Toxin Mechanisms and Immunotoxins
- Redox biology and oxidative stress
- Polyamine Metabolism and Applications
- Plant-Microbe Interactions and Immunity
- Glutathione Transferases and Polymorphisms
- Mycotoxins in Agriculture and Food
- Insect and Pesticide Research
Centre National de la Recherche Scientifique
2015-2024
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2019-2024
Institut Sophia Agrobiotech
2015-2024
Université Côte d'Azur
2013-2024
Observatoire de la Côte d’Azur
2018-2019
Institut de Biologie Valrose
1998-2017
Institut National de la Recherche Agronomique
2013-2014
Laboratoire de Recherche Scientifique
2012
Biologie et Génétique des Interactions Plante-Parasite
2012
Université d'Angers
2008
Legume-Rhizobium nitrogen fixation is dramatically affected under drought and other environmental constraints. However, it has yet to be established as whether such regulation of only exerted at the whole-plant level (e.g. by a systemic feedback mechanism) or can also occur local nodule level. To address this question, nodulated pea (Pisum sativum) plants were grown in split-root system, which allowed for half root system irrigated field capacity, while was water deprived, thus provoking...
Summary Reactive oxygen species ( ROS ), particularly hydrogen peroxide (H 2 O play an important role in signalling various cellular processes. The involvement of H the Medicago truncatula – Sinorhizobium meliloti symbiotic interaction raises questions about its effect on gene expression. A transcriptome analysis was performed inoculated roots M. which production inhibited with diphenylene iodonium DPI ). In total, 301 genes potentially regulated by content were identified d after...
Legume-Rhizobium nitrogen fixation is dramatically affected under drought and other environmental constraints. However, it has yet to be established as whether such regulation of only exerted at the whole-plant level (e.g. by a systemic feedback mechanism) or can also occur local nodule level. To address this question, nodulated pea (Pisum sativum) plants were grown in split-root system, which allowed for half root system irrigated field capacity, while was water deprived, thus provoking...
Legumes form a symbiotic interaction with bacteria of the Rhizobiaceae family to produce nitrogen-fixing root nodules under nitrogen-limiting conditions. This process involves recognition bacterial Nod factors by plant which mediates entry into and nodule organogenesis. We have examined importance low molecular weight thiols, glutathione (GSH) homoglutathione (hGSH), during nodulation in model legume Medicago truncatula. Using both buthionine sulfoximine, specific inhibitor GSH hGSH...
Silencing of a Nicotiana plumbaginifolia beta-1,3-glucanase (gn1) transgene in tobacco line T17 occurs homozygous and haploid plants with one locus dosage per chromosome set. We have previously shown that the silent state is manifested by reduced gn1 steady mRNA level results from post-transcriptional process under developmental control plants. In this study, we show specific endogenous genes are cosuppressed also demonstrate timing cosuppression depends on environmental conditions once...
ABSTRACT Rhizobia form a symbiotic relationship with plants of the legume family to produce nitrogen-fixing root nodules under nitrogen-limiting conditions. We have examined importance glutathione (GSH) during free-living growth and symbiosis Sinorhizobium meliloti . An S. mutant strain (SmgshA) which is unable synthesize GSH due gene disruption in gshA , encoding enzyme for first step biosynthesis GSH, was grow nonstress conditions, precluding any nodulation. In contrast, an (SmgshB) gshB...
Root-knot nematodes (RKN) are obligatory plant parasitic worms that establish and maintain an intimate relationship with their host plants. During a compatible interaction, RKN induce the redifferentiation of root cells into multinucleate hypertrophied giant essential for nematode growth reproduction. These metabolically active feeding constitute exclusive source nutrients nematode. Detailed analysis glutathione (GSH) homoglutathione (hGSH) metabolism demonstrated importance these compounds...
Climate change is increasingly impacting the water deficit over world. Because of drought and high pressure rising human population, becoming a scarce expensive commodity, especially in developing countries. The identification crops presenting higher acclimation to stress thus an important objective agriculture. present investigation aimed assess adaptation three Vicia faba genotypes, Aguadulce (AD), Luz d’Otonio (LO) Reina Mora (RM) deficit. Multiple physiological biochemical parameters...
ABSTRACT Two catalases, KatA and KatB, have been detected in Sinorhizobium meliloti growing on rich medium. Here we characterize a new catalase gene encoding third (KatC). KatC activity was detectable only at the end of stationary phase S. minimum medium, whereas found during exponential phase. Analysis with katC-lacZ fusion demonstrated that katC expression is mainly regulated transcription level. An increase correlating induction bacteroids. A dramatic decrease nitrogen fixation capacity...
Abstract Thioredoxins (Trxs) constitute a family of small proteins in plants. This has been extensively characterized Arabidopsis (Arabidopsis thaliana), which contains six different Trx types: f, m, x, and y chloroplasts, o mitochondria, h mainly cytosol. A detailed study this the model legume Medicago truncatula, realized here, established existence two isoforms that do not belong to any types previously described. As no possible orthologs were further found either rice (Oryza sativa) or...
• Legumes form a symbiotic interaction with bacteria of the Rhizobiaceae family to produce nitrogen-fixing root nodules under nitrogen-limiting conditions. We examined importance glutathione (GSH) and homoglutathione (hGSH) during nitrogen fixation process. Spatial patterns expression genes involved in biosynthesis both thiols were studied using promoter-GUS fusion analysis. Genetic approaches nodule zone-specific cysteine rich (NCR001) promoter employed determine (h)GSH biological (BNF)....
To investigate the involvement of bacterial catalases symbiotic gram-negative bacterium Rhizobium meliloti in development Medicago-Rhizobium functional nodules, we cloned a putative kat gene by screening cosmid library with catalase-specific DNA probe amplified PCR from R. genome. Nucleotide sequence analysis 1.8-kb fragment revealed an open reading frame, called katA, encoding peptide 562 amino acid residues calculated molecular mass 62.9 kDa. The predicted showed high homology primary...
Legumes interact symbiotically with bacteria of the Rhizobiaceae to form nitrogen-fixing root nodules. We investigated contribution three glutaredoxin (Grx)-encoding genes present in Sinorhizobium meliloti genome this symbiosis. SmGRX1 (CGYC active site) and SmGRX3 (CPYG) recombinant proteins displayed deglutathionylation activity 2-hydroethyldisulfide assay, whereas SmGRX2 (CGFS) did not. Mutation not affect S. growth or symbiotic capacities. In contrast, mutations decreased free-living...