A5072726180- Legume Nitrogen Fixing Symbiosis
- Nematode management and characterization studies
- Mycotoxins in Agriculture and Food
- DNA and Nucleic Acid Chemistry
- Plant Pathogens and Fungal Diseases
- Plant nutrient uptake and metabolism
- Plant Molecular Biology Research
- DNA Repair Mechanisms
- Plant Virus Research Studies
- Photosynthetic Processes and Mechanisms
- RNA and protein synthesis mechanisms
- Agriculture, Plant Science, Crop Management
- Plant and fungal interactions
- Plant tissue culture and regeneration
- Plant Disease Resistance and Genetics
- Agronomic Practices and Intercropping Systems
- Wheat and Barley Genetics and Pathology
- Microtubule and mitosis dynamics
- Plant Reproductive Biology
- Evolution and Genetic Dynamics
- HIV/AIDS drug development and treatment
- Plant Pathogenic Bacteria Studies
- Transgenic Plants and Applications
- Fungal Biology and Applications
- Biofuel production and bioconversion
Génétique, Diversité, Écophysiologie des Céréales
2012-2014
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2013-2014
Université Côte d'Azur
2008-2012
Institut National de la Recherche Agronomique
1998-2012
Centre National de la Recherche Scientifique
2007-2012
Institut Sophia Agrobiotech
2009
Département Santé des Plantes et Environnement
2008
Laboratoire des Interactions Plantes Micro-Organismes
1999-2005
Laboratoire Physiologie Cellulaire & Végétale
2004
University of Namur
1993
Summary During a compatible interaction, root‐knot nematodes ( Meloidogyne spp.) induce the redifferentiation of root cells into multinucleate nematode feeding (giant cells). Hyperplasia and hypertrophy surrounding leads to formation gall. We investigated plant response by carrying out global analysis gene expression during gall in Arabidopsis, using giant cell‐enriched tissues. Among 22 089 genes monitored with complete Arabidopsis transcriptome microarray gene‐specific tag, we identified...
Plant-parasitic nematodes Meloidogyne spp induce an elaborate permanent feeding site characterized by the redifferentiation of root cells into multinucleate and hypertrophied giant cells. We have isolated a promoter trap strategy Arabidopsis thaliana formin gene, AtFH6, which is upregulated during cell formation. Formins are actin-nucleating proteins that stimulate de novo polymerization actin filaments. show here three type-I formins were in AtFH6 protein was anchored to plasma membrane...
Abstract The infection of plants by obligate parasitic nematodes constitutes an interesting model for investigating plant cytoskeleton functions. Root knot have evolved the ability to manipulate host functions their own advantage redifferentiating root cells into multinucleate and hypertrophied feeding cells. These giant result from repeated rounds karyokinesis without cell division. Detailed functional analyses demonstrated that Arabidopsis thaliana Microtubule-Associated Protein65-3...
The establishment and development of plant-microorganism interactions involve impressive transcriptomic reprogramming target plant genes. symbiont (Sinorhizobium meliloti) the root knot-nematode pathogen (Meloidogyne incognita) induce formation new organs, nodule gall, respectively. Using laser-assisted microdissection, we specifically monitored, at cell level, Medicago gene expression in zone II cells, which are preparing to receive rhizobia, gall giant surrounding play an essential role...
In eukaryotes, the spindle assembly checkpoint (SAC) ensures that chromosomes undergoing mitosis do not segregate until they are properly attached to microtubules of spindle.We investigated mechanism underlying this surveillance in plants, by characterising orthogolous SAC proteins BUBR1, BUB3 and MAD2 from Arabidopsis. We showed cell cycle-regulated BUB3.1 interacted physically with each other. Furthermore, BUBR1 specifically at chromocenters. Following activation global defects assembly,...
Root-knot nematodes (RKN) are obligatory plant parasitic worms that establish and maintain an intimate relationship with their host plants. During a compatible interaction, RKN induce the redifferentiation of root cells into multinucleate hypertrophied giant essential for nematode growth reproduction. These metabolically active feeding constitute exclusive source nutrients nematode. Detailed analysis glutathione (GSH) homoglutathione (hGSH) metabolism demonstrated importance these compounds...
Plants associate with a wide range of mutualistic and parasitic biotrophic organisms. Here, we investigated whether beneficial plant symbionts pathogens induce distinct or overlapping regulatory pathways in Medicago truncatula. The symbiosis between Sinorhizobium meliloti this results the formation nitrogen-fixing root nodules requiring activation specific genes host plant. We studied expression patterns nodule-expressed after infection root-knot nematode Meloidogyne incognita. Two...
Root-knot nematodes (RKNs) are sedentary biotrophic parasites that induce the differentiation of root cells into feeding provide with nutrients necessary for their development. The development new control methods against RKNs relies greatly on functional analysis genes crucial pathogen or success parasitism. In absence genetic transformation, RNA interference (RNAi) allows phenotype nematode and establishment in its host after sequence-specific knock-down targeted genes. Strategies used to...
Abstract Background Carotenoids are isoprenoid pigments, essential for photosynthesis and photoprotection in plants. The enzyme phytoene synthase (PSY) plays an role mediating condensation of two geranylgeranyl diphosphate molecules, the first committed step carotenogenesis. PSY nuclear enzymes encoded by a small gene family consisting three paralogous genes ( 1-3) that have been widely characterized rice, maize sorghum. Results In wheat, which yellow pigment content is extremely important...
Summary The spindle assembly checkpoint ( SAC ) is a refined surveillance mechanism which ensures that chromosomes undergoing mitosis do not segregate until they are properly attached to the microtubules MT ). has been extensively studied in metazoans and yeast, but little known about its role plants. We identified proteins interacting with ‐associated protein MAP 65‐3, plays critical organising mitotic arrays, carried out functional analysis of previously newly components. show Arabidopsis...
Journal Article Mechanism of ultraviolet-induced mutagenesis: the coding properties ultraviolet-irradiated poly (dC) replicated by E. coli DNA polymerase I Get access Philippe Lecomte, Lecomte Department Molecular Biology, Université Libre de Bruxelles67 rue des Chevaux, B - 1640 Rhode-St-Genése, Belgium Search for other works this author on: Oxford Academic PubMed Google Scholar Serge Boiteux, Boiteux Oliver Doubleday Nucleic Acids Research, Volume 9, Issue 14, 24 July 1981, Pages...
ABSTRACT To characterize host genes required for a compatible interaction, we identified novel recessive Arabidopsis thaliana mutant, nws1 (no wilt symptoms), that failed to develop symptoms in response virulent strains of the phytopathogenic bacterium, Ralstonia solanacearum. The absence wilting plants was not correlated with cell death phenotype or constitutive expression salicylic acid-, jasmonic acid- ethylene-associated genes. In addition, this mutation, which conferred symptomless all...