A5023964145- Peptidase Inhibition and Analysis
- Biochemical and Structural Characterization
- Click Chemistry and Applications
- Renin-Angiotensin System Studies
- Antimicrobial Peptides and Activities
- Mosquito-borne diseases and control
- Protease and Inhibitor Mechanisms
- Chemical Synthesis and Analysis
- Receptor Mechanisms and Signaling
- Computational Drug Discovery Methods
- Phosphodiesterase function and regulation
- Neuropeptides and Animal Physiology
- Signaling Pathways in Disease
- Malaria Research and Control
- Ubiquitin and proteasome pathways
- Venomous Animal Envenomation and Studies
- Bacterial Genetics and Biotechnology
- HIV/AIDS drug development and treatment
- RNA and protein synthesis mechanisms
Universidade Federal de São Paulo
2020-2025
We describe a method tailored to the in-cell selection of protease inhibitors. In this method, target is co-expressed with selective substrate, product which kills host cells. Therefore, can be applied identify potential inhibitors based on cell survival when inhibition occurs. The TEV was chosen for proof-of-concept experiment. genetically encoded substrate single polypeptide chain composed three parts: (1) ccdB protein, cause death it accumulates inside cell; (2) cleavage sequence that...
The aggregation of α-synuclein (α-Syn) is a characteristic Parkinson’s disease (PD). α-Syn oligomerization/aggregation accelerated by the serine peptidase, prolyl oligopeptidase (POP). Factors that affect POP conformation, including most its inhibitors and an impairing mutation in active site, influence acceleration resulting from interaction these proteins. It noteworthy, however, not cleaved POP. Prolyl endopeptidase-like (PREPL) protein structurally related to peptidases belonging family....
Crotamine is a highly cationic polypeptide first isolated from South American rattlesnake venom, which exhibits affinity for acidic lysosomal vesicles and proliferating cells. This nature pivotal its in vitro cytotoxicity vivo anticancer actions. study aimed to enhance the antitumor efficacy of crotamine by associating it with mesoporous SBA-15 silica, known controlled release various chemical agents, including large proteins. association mitigate toxic effects while amplifying...
We are describing a method tailored for in-cell selection of protease inhibitors. In this method, target is co-expressed with selective substrate, the product which kills host cells. Therefore, it can be applied to identify potential inhibitors, based on cell survival, when inhibition occurs. The would suitable inhibitors from intracellularly generated inhibitor libraries, like SICLOPPS, instance. TEV was chosen proof-of-concept. genetically encoded substrate single polypeptide chain,...