A5005239660- Venomous Animal Envenomation and Studies
- Antimicrobial Peptides and Activities
- Insect and Pesticide Research
- RNA Interference and Gene Delivery
- Neuropeptides and Animal Physiology
- Peptidase Inhibition and Analysis
- Biochemical and Structural Characterization
- Nicotinic Acetylcholine Receptors Study
- Malaria Research and Control
- Adipose Tissue and Metabolism
- Renal and related cancers
- Organ Donation and Transplantation
- Exercise and Physiological Responses
- Cardiac Structural Anomalies and Repair
- Protease and Inhibitor Mechanisms
- Genetics, Aging, and Longevity in Model Organisms
- Gold and Silver Nanoparticles Synthesis and Applications
- Extracellular vesicles in disease
- Autophagy in Disease and Therapy
- Cancer Cells and Metastasis
- Cancer-related gene regulation
- Histone Deacetylase Inhibitors Research
- Mitochondrial Function and Pathology
- Developmental Biology and Gene Regulation
- Renal Diseases and Glomerulopathies
Erasmus MC
2024-2025
Erasmus University Rotterdam
2024-2025
Union des Industries Ferroviaires Européennes
2024
Universidade Federal de São Paulo
2015-2023
University Medical Center Groningen
2022-2023
University of Groningen
2022-2023
Instituto Butantan
2010
Abstract Background Fibroblast activation protein (FAP), a transmembrane serine protease overexpressed by cancer-associated fibroblasts in the tumor stroma, is an interesting biomarker for targeted radionuclide theranostics. FAP-targeting radiotracers have demonstrated to be superior [ 18 F]FDG PET/CT various solid cancers. However, these suboptimal retention therapy (TRT). We aimed develop novel pharmacophore with improved pharmacokinetics introducing substitution at 8-position of...
Crotamine is a natural polypeptide from snake venom which delivers nucleic acid molecules into cells, besides having pronounced affinity for negatively charged membranes and antifungal activity. We previously demonstrated that crotamine derived short linear peptides were not very effective as antifungal, although the non-structured recombinant was overridingly more potent compared to native structured crotamine. Aiming identify features necessary activity of crotamine, two peptides, each...
The high medical importance of Crotalus snakes is unquestionable, as this genus the second in frequency ophidian accidents many countries, including Brazil. With a relative less complex composition compared to other genera venoms, those from Bothrops genus, venom South America composed basically by neurotoxin crotoxin (a phospholipase A2), thrombin-like gyroxin serinoprotease), very potent aggregating protein convulxin, and myotoxic polypeptide named crotamine. Interestingly not all express...
Crotamine, originally isolated from rattlesnake venom, has been extensively studied due to its pleiotropic biological properties, and special attention paid antitumor activity. However, long-term treatment with crotamine was accompanied by a reduction in animal body weight gain increases glucose tolerance. As cancer is commonly associated cachexia, preclude the possible cachexia-like effect of crotamine, herein this polypeptide administered healthy wild-type C57/BL6 mice oral route daily,...
Abstract Activated proximal tubular epithelial cells (PTECs) play a crucial role in progressive tubulo-interstitial fibrosis native and transplanted kidneys. Targeting PTECs by non-viral delivery vectors might be useful to influence the expression of important genes and/or proteins order slow down renal function loss. However, no clinical therapies that specifically target are available at present. We earlier showed cationic cell penetrating peptide isolated from South American rattlesnake...
Proteinuria drives progressive tubulointerstitial fibrosis in native and transplanted kidneys, mainly through the activation of proximal tubular epithelial cells (PTECs). During proteinuria, PTEC syndecan-1 functions as a docking platform for properdin-mediated alternative complement activation. Non-viral gene delivery vectors to target could be useful slow down In this work, we characterize PTEC-specific non-viral vector composed cell-penetrating peptide crotamine complexed with targeting...
Mitochondria have pivotal roles in cellular physiology including energy metabolism, reactive oxygen species production, Ca2+ homeostasis, and apoptosis. Altered mitochondrial morphology function is a common feature of cancer cells the regulation homeostasis has been identified as key to response chemotherapeutic agents human leukemias. Here, we explore mechanistic aspects cytotoxicity produced by thioridazine (TR), an antipsychotic drug that investigated for its anticancer potential leukemia...
Crotamine is a cationic polypeptide composed by 42 amino acid residues with several pharmacological and biological properties, including the selective ability to enter kill actively proliferating tumour cells, which led us propose its use as theranostic agent for cancer therapy. At moment, improvement of crotamine antitumoral efficacy association chemotherapeutic adjuvants envisioned. In present work, we evaluated adjuvant phenotiazine thioridazine (THD). spite clear these both compounds...
Although tyrosine kinase inhibitors have revolutionized treatment for chronic myeloid leukemia (CML), some patients exhibit drug resistance and poor clinical outcomes. Here, we explore the mechanistic aspects of cytotoxicity produced by thioridazine (TR). TR is an antipsychotic that has been investigated its anticancer potential in a Philadelphia-positive human CML model. Our data show selectively induced apoptosis K562 cells compared with normal blood cells, which was mediated Ca2+...
Abstract Background Fibroblast activation protein (FAP) is an attractive target for cancer theranostics. Although FAP-targeted nuclear imaging demonstrated promising clinical results, only sub-optimal results are reported targeted radionuclide therapy (TRT). Preclinical research crucial in selecting radiopharmaceuticals and obtaining increased understanding of factors essential FAP-TRT improvement. FAP mainly expressed by cancer-associated fibroblasts the tumor stroma less on cells...