A5004866942- Acute Myeloid Leukemia Research
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Chronic Myeloid Leukemia Treatments
- Acute Lymphoblastic Leukemia research
- Chronic Lymphocytic Leukemia Research
- Protein Degradation and Inhibitors
- Autophagy in Disease and Therapy
- Microtubule and mitosis dynamics
- Histone Deacetylase Inhibitors Research
- Retinoids in leukemia and cellular processes
- Kruppel-like factors research
- Multiple Myeloma Research and Treatments
- Ubiquitin and proteasome pathways
- Cell death mechanisms and regulation
- Epigenetics and DNA Methylation
- Hematopoietic Stem Cell Transplantation
- Hemoglobinopathies and Related Disorders
- Cytokine Signaling Pathways and Interactions
- Cancer-related Molecular Pathways
- Cancer therapeutics and mechanisms
- HIV/AIDS drug development and treatment
- PI3K/AKT/mTOR signaling in cancer
- Hematological disorders and diagnostics
- Synthesis and Biological Activity
- Phagocytosis and Immune Regulation
Universidade de São Paulo
2016-2025
Hospital Universitário da Universidade de São Paulo
2022-2024
Universidade Federal de São Carlos
2023
Universidade de Ribeirão Preto
2016-2022
Universidade Estadual de Campinas (UNICAMP)
2010-2018
Cell-cell adhesions and the cytoskeletons play important coordinated roles in cell biology, including differentiation, development, migration. Adhesion cytoskeletal dynamics are regulated by Rho-GTPases. ARHGAP21 is a negative regulator of Rho-GTPases, particularly Cdc42. Here we assess function cell-cell adhesion, migration, scattering. We find that localized nucleus, cytoplasm, or perinuclear region but transiently redistributed to junctions 4 h after initiation adhesion. interacts with...
ABSTRACT The human FMNL1 is expressed predominantly in hematopoietic cells and has been described previously as overexpressed malignancies. However, it not known whether contributes to leukemogenesis. Here, we investigate the function using two different leukemia models: Namalwa K562 cell lines. depletion reduced proliferation colony formation both leukemic types, well a decrease tumor growth of FMNL1-depleted xenografts. In addition, there was migration TEM cells. endogenously associates...
Mastocytosis are myeloproliferative neoplasms commonly related to gain-of-function mutations involving the tyrosine kinase domain of KIT. We herein report a case familial systemic mastocytosis with rare KIT K509I germ line mutation affecting two family members: mother and daughter. In vitro treatment imatinib, dasatinib PKC412 reduced cell viability primary mast cells harboring mutation. However, imatinib was more effective in inducing apoptosis neoplastic cells. Both patients had remarkable...
The IGF1R/IRS1 signaling is activated in acute lymphoblastic leukemia (ALL) and can be targeted by the pharmacological inhibitors NT157 (IGF1R-IRS1/2 inhibitor) OSI-906 (IGF1R/IR inhibitor). Here we investigate cellular molecular effects of ALL cells. treatment reduced viability, proliferation cell cycle progression lines. Similarly, primary samples patients with ALL, both but only induced apoptosis. did not show cytotoxicity from healthy donor. significantly decreased Jurkat migration,...
Hemoglobin SC disease is a very prevalent hemoglobinopathy; however, little known about this condition specifically. There appears to be an increased risk of thromboembolic events in hemoglobin disease, but studies evaluating the hemostatic alterations are lacking. We describe findings cross-sectional observational study coagulation activation markers adult patients with SC, comparing them those sickle cell anemia and healthy controls. A total 56 39 were included study, all steady state, 27...
Abstract Recent data indicate that IGF1R/IRS signaling is a potential therapeutic target in BCR-ABL1-negative myeloproliferative neoplasms (MPN); this pathway, IRS2 involved the malignant transformation induced by JAK2 V617F , and upregulation of IGF1R induces MPN phenotype. NT157, synthetic compound designed as an IGF1R-IRS1/2 inhibitor, has been shown to induce antineoplastic effects solid tumors. Herein, we aimed characterize molecular cellular NT157 -positive cell lines (HEL SET2)...
The need of pharmacological strategies to preclude breast cancer development motivated us develop a non-aqueous microemulsion (ME) capable forming depot after administration in the mammary tissue and uptake interstitial fluids for prolonged release retinoid fenretinide. selected ME was composed phosphatidylcholine/tricaprylin/propylene glycol (45:5:50, w/w/w) presented droplet diameter 175.3 ± 8.9 nm. Upon water uptake, transformed successively into lamellar phase, gel, phase-containing...
Abstract The treatment of acute leukemia is challenging because the genetic heterogeneity between and within patients. Leukemic stem cells (LSCs) are relatively drug-resistant frequently relapse. Their plasticity capacity to adapt extracellular stress, in which mitochondrial metabolism autophagy play important roles, further complicates treatment. Genetic models phosphatidylinositol-5-phosphate 4-kinase type 2 protein (PIP4K2s) inhibition have demonstrated relevance these enzymes homeostasis...
ANKHD1 is a multiple ankyrin repeat containing protein, highly expressed in cancers, such as acute leukemia. The present study was undertaken to determine the expression and functional significance of human Multiple Myeloma (MM). We found that MM patient cells cell lines. In vitro , lentiviral mediated ANKHD1‐shRNA inhibited proliferation delayed S G2M cycle progression glucocorticoid resistant (U266) sensitive (MM1S) cells. Further silencing resulted upregulation cyclin dependent kinase...
The interaction between the bone marrow microenvironment and malignant hematopoietic cells can result in protection of leukemia from chemotherapy both myelodysplastic syndromes (MDS) acute myeloid (AML). We, herein, characterized changes cytokine expression function mesenchymal stromal (MSC) patients with MDS, AML myelodysplasia-related (MRC), a well-recognized clinical subtype secondary AML, de novo AML. We observed significant inhibitory effect MDS-MSC on T lymphocyte proliferation no...
The role of the immune system in myelodysplastic syndrome (MDS) progression has been widely accepted, although mechanisms underlying this dysfunction are not clear. CD4+ and CD8+ lymphocyte profiles peripheral blood MDS patients were evaluated correlated with clinical characteristics, expression FOXP3 anti-inflammatory cytokines IL10, TGFβ1 CTLA4. IL10 inversely percentage cells was higher high-risk MDS. Our findings provide further evidence for T cell-mediated production strengthen idea...
BCR-ABL kinase activates downstream signaling pathways, including the PI3K-Akt/mTOR and MAPK pathway. IRS1 has been previously described as constitutively phosphorylated associated with in K562 cells, suggesting that role pathways. In this study, we analyzed effect of silencing, by shRNA-lentiviral delivery, a CML cell line presents BCR-ABL. silencing decreased proliferation colony formation which correlates delay these cells at G0/G1 phase decrease S cycle. Furthermore, resulted Akt, P70S6K...